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Literature summary extracted from

  • Garrity-Ryan, L.; Shafikhani, S.; Balachandran, P.; Nguyen, L.; Oza, J.; Jakobsen, T.; Sargent, J.; Fang, X.; Cordwell, S.; Matthay, M.A.; Engel, J.N.
    The ADP ribosyltransferase domain of Pseudomonas aeruginosa ExoT contributes to its biological activities (2004), Infect. Immun., 72, 546-558.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
2.4.2.30 additional information enzyme is comprised of an N-terminal domain with GTPase activating protein activity towards Rho family GTPases and a C-terminal ADP ribosyl-transferase (ADPRT) domain with minimal activity towards a synthetic substrate in vitro. Deletion of a majority of the ADPRT domain (residues 234 to 438) or point mutations of the ADPRT catalytic site (residues 383 to 385) leads to distinct changes in host cell morphology and substantially reduces the ability of ExoT to inhibit in vitro epithelial wound healing over a 24-h period. In contrast, only subtle effects on the efficiency of ExoT-induced bacterial internalization are observed in the ADPRT mutant forms Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.30 NAD+ + (ADP-D-ribosyl)n-acceptor Pseudomonas aeruginosa ADPRT domain of ExoT is active in vivo and contributes to the pathogenesis of Pseudomonas aeruginosa infections nicotinamide + (ADP-D-ribosyl)n+1-acceptor
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Organism

EC Number Organism UniProt Comment Textmining
2.4.2.30 Pseudomonas aeruginosa
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.30 NAD+ + (ADP-D-ribosyl)n-acceptor ADPRT domain of ExoT is active in vivo and contributes to the pathogenesis of Pseudomonas aeruginosa infections Pseudomonas aeruginosa nicotinamide + (ADP-D-ribosyl)n+1-acceptor
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Synonyms

EC Number Synonyms Comment Organism
2.4.2.30 ExoT enzyme is comprised of an N-terminal domain with GTPase activating protein activity towards Rho family GTPases and a C-terminal ADP ribosyl-transferase (ADPRT) domain with minimal activity towards a synthetic substrate in vitro Pseudomonas aeruginosa