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Literature summary extracted from

  • Mok, M.T.; Tay, E.; Sekyere, E.; Glenn, W.K.; Bagnara, A.S.; Edwards, M.R.
    Giardia intestinalis: molecular characterization of UDP-N-acetylglucosamine pyrophosphorylase (2005), Gene, 357, 73-82.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.23 expressed as His-tag fusion protein in Escherichia coli M15 Giardia intestinalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.23 48290
-
 calculated from the deduced amino acid sequence Giardia intestinalis
2.7.7.23 49624
-
 x * 49624, calculated from the deduced amino acid sequence of the His-tagged protein Giardia intestinalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.23 UTP + N-acetyl-alpha-D-glucosamine 1-phosphate Giardia intestinalis involved in the synthesis of UDP-N-acetyl-galactoseamine, which is required for cyst wall synthesis diphosphate + UDP-N-acetyl-D-glucosamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.23 Giardia intestinalis Q8MUP8 Portland 1 strain, ATCC30888
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.23 recombinant protein using His-tag Giardia intestinalis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.23 39.3
-
 purified recombinant enzyme Giardia intestinalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.23 UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 Giardia intestinalis diphosphate + UDP-N-acetyl-D-glucosamine
-
 ?
2.7.7.23 UTP + N-acetyl-alpha-D-glucosamine 1-phosphate involved in the synthesis of UDP-N-acetyl-galactoseamine, which is required for cyst wall synthesis Giardia intestinalis diphosphate + UDP-N-acetyl-D-glucosamine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.23 ? x * 49624, calculated from the deduced amino acid sequence of the His-tagged protein Giardia intestinalis