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Literature summary extracted from

  • Comini, M.A.; Guerrero, S.A.; Haile, S.; Menge, U.; Lunsdorf, H.; Flohe, L.
    Valdiation of Trypanosoma brucei trypanothione synthetase as drug target (2004), Free Radic. Biol. Med., 36, 1289-1302.
    View publication on PubMed

Application

EC Number Application Comment Organism
6.3.1.8 drug development the enzyme is a target for drug development Trypanosoma brucei
6.3.1.9 drug development the enzyme is a target for drug development Trypanosoma brucei

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.1.8 enzyme expression analysis, cloning and transfection of RNAi Trypanosoma brucei
6.3.1.9 enzyme expression analysis, cloning and transfection of RNAi Trypanosoma brucei

Protein Variants

EC Number Protein Variants Comment Organism
6.3.1.8 additional information enzyme downregulation leads to increased trypanocidic drug sensitivity, enzyme knockdown leads to depletion of both trypanothione and glutathionylspermidine and glutathione accumulation, RNA interference method, overview Trypanosoma brucei
6.3.1.9 additional information enzyme downregulation leads to increased trypanocidic drug sensitivity, enzyme knockdown leads to depletion of both trypanothione and glutathionylspermidine and glutathione accumulation, RNA interference method, overview Trypanosoma brucei

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.1.8 buthionine sulfoximine
-
 Trypanosoma brucei
6.3.1.8 megazol
-
 Trypanosoma brucei
6.3.1.8 Melarsen oxide
-
 Trypanosoma brucei
6.3.1.8 additional information the enzyme mediates resistance to H2O2 and organic hyperoxids, and to trypanocidic drugs Trypanosoma brucei
6.3.1.8 nifurtimox
-
 Trypanosoma brucei
6.3.1.8 Pentamidine
-
 Trypanosoma brucei
6.3.1.8 triostam
-
 Trypanosoma brucei
6.3.1.9 buthionine sulfoximine
-
 Trypanosoma brucei
6.3.1.9 megazol
-
 Trypanosoma brucei
6.3.1.9 Melarsen oxide
-
 Trypanosoma brucei
6.3.1.9 additional information the enzyme mediates resistance to H2O2 and organic hyperoxids, and to trypanocidic drugs Trypanosoma brucei
6.3.1.9 nifurtimox
-
 Trypanosoma brucei
6.3.1.9 Pentamidine
-
 Trypanosoma brucei
6.3.1.9 triostam
-
 Trypanosoma brucei

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.1.8 Mg2+
-
 Trypanosoma brucei
6.3.1.9 Mg2+
-
 Trypanosoma brucei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.1.8 glutathione + spermidine + ATP Trypanosoma brucei trypanothione synthase catalyzes both the reaction of glutathionylspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9, in Trypanosoma brucei glutathionylspermidine + ADP + phosphate
-
 ?
6.3.1.8 additional information Trypanosoma brucei the enzyme is important for several functions, e.g. for detoxification of H2O2 and organic hyperoxids, and ribonucleotide reduction ?
-
 ?
6.3.1.9 2 glutathione + spermidine + 2 ATP Trypanosoma brucei trypanothione synthase overall reaction, trypanothione metabolism overview N1,N8-bis(glutathionyl)spermidine + 2 ADP + 2 phosphate
-
 ?
6.3.1.9 glutathione + glutathionylspermidine + ATP Trypanosoma brucei trypanothione synthase catalyzes both the reaction of glutathionylspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9, in Trypanosoma brucei N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
 ?
6.3.1.9 glutathione + spermidine + ATP Trypanosoma brucei trypanothione synthase catalyzes both the reaction of glutathionylspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9, in Trypanosoma brucei glutathionylspermidine + ADP + phosphate
-
 ?
6.3.1.9 additional information Trypanosoma brucei the enzyme is important for several functions, e.g. for detoxification of H2O2 and organic hyperoxids, and ribonucleotide reduction ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.8 Trypanosoma brucei
-
 bifunctional enzyme with glutathionylspermidine synthase and trypanothione synthase activity
-
6.3.1.9 Trypanosoma brucei
-
 bifunctional enzyme with glutathionylspermidine synthase and trypanothione synthase activity
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.8 glutathione + spermidine + ATP trypanothione synthase catalyzes both the reaction of glutathionylspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9, in Trypanosoma brucei Trypanosoma brucei glutathionylspermidine + ADP + phosphate
-
 ?
6.3.1.8 additional information the enzyme is important for several functions, e.g. for detoxification of H2O2 and organic hyperoxids, and ribonucleotide reduction Trypanosoma brucei ?
-
 ?
6.3.1.9 2 glutathione + spermidine + 2 ATP trypanothione synthase overall reaction Trypanosoma brucei N1,N8-bis(glutathionyl)spermidine + 2 ADP + 2 phosphate
-
 ?
6.3.1.9 2 glutathione + spermidine + 2 ATP trypanothione synthase overall reaction, trypanothione metabolism overview Trypanosoma brucei N1,N8-bis(glutathionyl)spermidine + 2 ADP + 2 phosphate
-
 ?
6.3.1.9 glutathione + glutathionylspermidine + ATP trypanothione synthase catalyzes both the reaction of glutathionylspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9, in Trypanosoma brucei Trypanosoma brucei N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
 ?
6.3.1.9 glutathione + glutathionylspermidine + ATP trypanothione synthase catalyzes both the reaction of glutathionyspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9, in Trypanosoma brucei Trypanosoma brucei N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
 ?
6.3.1.9 glutathione + spermidine + ATP trypanothione synthase catalyzes both the reaction of glutathionylspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9, in Trypanosoma brucei Trypanosoma brucei glutathionylspermidine + ADP + phosphate
-
 ?
6.3.1.9 additional information the enzyme is important for several functions, e.g. for detoxification of H2O2 and organic hyperoxids, and ribonucleotide reduction Trypanosoma brucei ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.1.9 TryS
-
 Trypanosoma brucei

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.1.8 ATP
-
 Trypanosoma brucei
6.3.1.9 ATP
-
 Trypanosoma brucei