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Literature summary extracted from

  • Voronovsky, A.Y.; Ryabova, O.B.; Verba, O.V.; Ishchuk, O.P.; Dmytruk, K.V.; Sibirny, A.A.
    Expression of xylA genes encoding xylose isomerases from Escherichia coli and Streptomyces coelicolor in the methylotrophic yeast Hansenula polymorpha (2005), FEMS Yeast Res., 5, 1055-1062.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.5 expression in the yeast Hansenula polymorpha Escherichia coli
5.3.1.5 expression in the yeast Hansenula polymorpha Streptomyces coelicolor

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.5 46000
-
 SDS-PAGE Escherichia coli
5.3.1.5 46000
-
 SDS-PAGE Streptomyces coelicolor

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.1.5 D-Xylose Escherichia coli
-
 D-Xylulose
-
 ?
5.3.1.5 D-Xylose Streptomyces coelicolor
-
 D-Xylulose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.5 Escherichia coli
-
 recombinant enzyme from Hansenula polymorpha transformants
-
5.3.1.5 Streptomyces coelicolor
-
 recombinant enzyme from Hansenula polymorpha transformants
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.5
-
 Escherichia coli
5.3.1.5
-
 Streptomyces coelicolor

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.1.5 0.012
-
 transformant delta-xyl1No.4(pEc)No.2b Escherichia coli
5.3.1.5 0.013
-
 transformant delta-xyl1No.4(pEc)No.2a Escherichia coli
5.3.1.5 0.014
-
 transformant delta-xyl1No.4(pScoel)No.4 Streptomyces coelicolor
5.3.1.5 0.015
-
 transformant delta-xyl1No.4(pScoel)No.12 Streptomyces coelicolor
5.3.1.5 0.065
-
 Escherichia coli wild-type Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.5 D-fructose
-
 Escherichia coli D-glucose
-
 r
5.3.1.5 D-fructose
-
 Streptomyces coelicolor D-glucose
-
 r
5.3.1.5 D-Xylose
-
 Escherichia coli D-Xylulose
-
 ?
5.3.1.5 D-Xylose
-
 Streptomyces coelicolor D-Xylulose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.3.1.5 xylose isomerase
-
 Escherichia coli
5.3.1.5 xylose isomerase
-
 Streptomyces coelicolor