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Literature summary extracted from

  • Yamamoto, S.; Suemoto, Y.; Seito, Y.; Nakao, H.; Shinoda, S.
    The presence of L-2,4-diaminobutyric acid decarboxylase activity in Vibrio species: a new biosynthetic pathway for 1,3-diaminopropane (1986), FEMS Microbiol. Lett., 35, 289-293.
No PubMed abstract available

General Stability

EC Number General Stability Organism
4.1.1.86 bovine serum albumin stabilizes at below 0.1 mg/ml Vibrio alginolyticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.86 Co2+ highly inhibitory at 20 mM Vibrio alginolyticus
4.1.1.86 Cu2+ highly inhibitory at 20 mM Vibrio alginolyticus
4.1.1.86 Fe2+ highly inhibitory at 20 mM Vibrio alginolyticus
4.1.1.86 Mn2+ highly inhibitory at 20 mM Vibrio alginolyticus
4.1.1.86 additional information no inhibition by N-ethylmaleimide at up to 20 mM Vibrio alginolyticus
4.1.1.86 Pb2+ highly inhibitory at 20 mM Vibrio alginolyticus
4.1.1.86 Sn2+ highly inhibitory at 20 mM Vibrio alginolyticus
4.1.1.86 Zn2+ highly inhibitory at 20 mM Vibrio alginolyticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.86 0.13
-
 L-2,4-diaminobutanoate pH 8.3, 37°C Vibrio alginolyticus
4.1.1.86 0.3
-
 DL-2,4-diaminobutanoate pH 8.3, 37°C Vibrio alginolyticus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.86 Mg2+
-
 Salinivibrio costicola
4.1.1.86 Mg2+
-
 Vibrio proteolyticus
4.1.1.86 Mg2+
-
 Vibrio cholerae serotype O1
4.1.1.86 Mg2+
-
 Vibrio anguillarum
4.1.1.86 Mg2+ 30% activation at 20 mM Vibrio alginolyticus
4.1.1.86 additional information enzyme is not affected by Ca2+ Vibrio alginolyticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.86 L-2,4-diaminobutanoate Salinivibrio costicola
-
 propane-1,3-diamine + CO2
-
 ?
4.1.1.86 L-2,4-diaminobutanoate Vibrio proteolyticus
-
 propane-1,3-diamine + CO2
-
 ?
4.1.1.86 L-2,4-diaminobutanoate Vibrio cholerae serotype O1
-
 propane-1,3-diamine + CO2
-
 ?
4.1.1.86 L-2,4-diaminobutanoate Vibrio alginolyticus
-
 propane-1,3-diamine + CO2
-
 ?
4.1.1.86 L-2,4-diaminobutanoate Vibrio anguillarum
-
 propane-1,3-diamine + CO2
-
 ?
4.1.1.86 L-2,4-diaminobutanoate Vibrio alginolyticus ATCC 17749
-
 propane-1,3-diamine + CO2
-
 ?
4.1.1.86 L-2,4-diaminobutanoate Vibrio anguillarum NCMB6
-
 propane-1,3-diamine + CO2
-
 ?
4.1.1.86 L-2,4-diaminobutanoate Vibrio proteolyticus ATCC 15338
-
 propane-1,3-diamine + CO2
-
 ?
4.1.1.86 L-2,4-diaminobutanoate Vibrio cholerae serotype O1 NCTC4716
-
 propane-1,3-diamine + CO2
-
 ?
4.1.1.86 L-2,4-diaminobutanoate Salinivibrio costicola NCMB701
-
 propane-1,3-diamine + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.86 Salinivibrio costicola
-
-
-
4.1.1.86 Salinivibrio costicola NCMB701
-
-
-
4.1.1.86 Vibrio alginolyticus
-
 ATCC17749
-
4.1.1.86 Vibrio alginolyticus ATCC 17749
-
 ATCC17749
-
4.1.1.86 Vibrio anguillarum
-
-
-
4.1.1.86 Vibrio anguillarum NCMB6
-
-
-
4.1.1.86 Vibrio cholerae serotype O1
-
 Non-O1
-
4.1.1.86 Vibrio cholerae serotype O1 NCTC4716
-
 Non-O1
-
4.1.1.86 Vibrio proteolyticus
-
 strain ATCC15338
-
4.1.1.86 Vibrio proteolyticus ATCC 15338
-
 strain ATCC15338
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.86 native enzyme 22.7fold by ammonium sulfate fractionation and anion exchange chromatography Vibrio alginolyticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.86 0.00036
-
 cell extract Vibrio anguillarum
4.1.1.86 0.00071
-
 cell extract Vibrio cholerae serotype O1
4.1.1.86 0.0011
-
 cell extract Vibrio proteolyticus
4.1.1.86 0.0014
-
 cell extract Salinivibrio costicola
4.1.1.86 29.5
-
 partially purified enzyme Vibrio alginolyticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.86 DL-2,4-diaminobutanoate
-
 Vibrio alginolyticus propane-1,3-diamine + CO2
-
 ?
4.1.1.86 DL-2,4-diaminobutanoate
-
 Vibrio alginolyticus ATCC 17749 propane-1,3-diamine + CO2
-
 ?
4.1.1.86 L-2,4-diaminobutanoate
-
 Salinivibrio costicola propane-1,3-diamine + CO2
-
 ?
4.1.1.86 L-2,4-diaminobutanoate
-
 Vibrio proteolyticus propane-1,3-diamine + CO2
-
 ?
4.1.1.86 L-2,4-diaminobutanoate
-
 Vibrio cholerae serotype O1 propane-1,3-diamine + CO2
-
 ?
4.1.1.86 L-2,4-diaminobutanoate
-
 Vibrio alginolyticus propane-1,3-diamine + CO2
-
 ?
4.1.1.86 L-2,4-diaminobutanoate
-
 Vibrio anguillarum propane-1,3-diamine + CO2
-
 ?
4.1.1.86 L-2,4-diaminobutanoate stereospecific reaction, no activity with the D-isomer Vibrio alginolyticus propane-1,3-diamine + CO2 product identification ?
4.1.1.86 L-2,4-diaminobutanoate
-
 Vibrio alginolyticus ATCC 17749 propane-1,3-diamine + CO2
-
 ?
4.1.1.86 L-2,4-diaminobutanoate stereospecific reaction, no activity with the D-isomer Vibrio alginolyticus ATCC 17749 propane-1,3-diamine + CO2 product identification ?
4.1.1.86 L-2,4-diaminobutanoate
-
 Vibrio anguillarum NCMB6 propane-1,3-diamine + CO2
-
 ?
4.1.1.86 L-2,4-diaminobutanoate
-
 Vibrio proteolyticus ATCC 15338 propane-1,3-diamine + CO2
-
 ?
4.1.1.86 L-2,4-diaminobutanoate
-
 Vibrio cholerae serotype O1 NCTC4716 propane-1,3-diamine + CO2
-
 ?
4.1.1.86 L-2,4-diaminobutanoate
-
 Salinivibrio costicola NCMB701 propane-1,3-diamine + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.1.86 DABA decarboxylase
-
 Salinivibrio costicola
4.1.1.86 DABA decarboxylase
-
 Vibrio proteolyticus
4.1.1.86 DABA decarboxylase
-
 Vibrio cholerae serotype O1
4.1.1.86 DABA decarboxylase
-
 Vibrio alginolyticus
4.1.1.86 DABA decarboxylase
-
 Vibrio anguillarum
4.1.1.86 L-2,4-diaminobutyric acid decarboxylase
-
 Salinivibrio costicola
4.1.1.86 L-2,4-diaminobutyric acid decarboxylase
-
 Vibrio proteolyticus
4.1.1.86 L-2,4-diaminobutyric acid decarboxylase
-
 Vibrio cholerae serotype O1
4.1.1.86 L-2,4-diaminobutyric acid decarboxylase
-
 Vibrio alginolyticus
4.1.1.86 L-2,4-diaminobutyric acid decarboxylase
-
 Vibrio anguillarum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.86 37
-
 assay at Salinivibrio costicola
4.1.1.86 37
-
 assay at Vibrio proteolyticus
4.1.1.86 37
-
 assay at Vibrio cholerae serotype O1
4.1.1.86 37
-
 assay at Vibrio anguillarum
4.1.1.86 45
-
-
 Vibrio alginolyticus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.86 37
-
 completely stable for up to 90 min Vibrio alginolyticus
4.1.1.86 45
-
 loss of 18% and 55% after 30 min and 2 h, respectively Vibrio alginolyticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.86 8 8.5
-
 Vibrio alginolyticus
4.1.1.86 8.3
-
 assay at Salinivibrio costicola
4.1.1.86 8.3
-
 assay at Vibrio proteolyticus
4.1.1.86 8.3
-
 assay at Vibrio cholerae serotype O1
4.1.1.86 8.3
-
 assay at Vibrio anguillarum

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.86 pyridoxal 5'-phosphate
-
 Salinivibrio costicola
4.1.1.86 pyridoxal 5'-phosphate
-
 Vibrio proteolyticus
4.1.1.86 pyridoxal 5'-phosphate
-
 Vibrio cholerae serotype O1
4.1.1.86 pyridoxal 5'-phosphate
-
 Vibrio alginolyticus
4.1.1.86 pyridoxal 5'-phosphate
-
 Vibrio anguillarum