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Literature summary extracted from

  • Kurihara, S.; Oda, S.; Kumagai, H.; Suzuki, H.
    gamma-Glutamyl-gamma-aminobutyrate hydrolase in the putrescine utilization pathway of Escherichia coli K-12 (2006), FEMS Microbiol. Lett., 256, 318-323.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.5.1.94 stabilization of PuuD activity by beta-mercaptoethanol Escherichia coli K-12

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.94 28500
-
 2 * 28500, calcaulation from amino-acid sequence Escherichia coli K-12
3.5.1.94 57000
-
 gel filtration Escherichia coli K-12

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.94 4-(gamma-glutamylamino)butanoate + H2O Escherichia coli K-12 enzyme is involved in the gamma-glutamyl-gamma-aminobutyrate hydrolase pathway for the degradation of putrescine to succinate semialdehyde. PuuD is essential for growth on putrescine as a sole source of nitrogen. Expression of the enzyme is induced by putrescine and O2. Addition of succinate and NH4Cl to the medium reduces the expression of PuuD 4-aminobutanoate + L-glutamate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.94 Escherichia coli K-12 P76038
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.94
-
 Escherichia coli K-12

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.94 additional information enzyme is expressed most highly in early stationary phase. The expression of puuD is dramatically repressed when Escherichia coli is grown up with low aeration Escherichia coli K-12
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.94 4-(gamma-glutamylamino)butanoate + H2O enzyme is involved in the gamma-glutamyl-gamma-aminobutyrate hydrolase pathway for the degradation of putrescine to succinate semialdehyde. PuuD is essential for growth on putrescine as a sole source of nitrogen. Expression of the enzyme is induced by putrescine and O2. Addition of succinate and NH4Cl to the medium reduces the expression of PuuD Escherichia coli K-12 4-aminobutanoate + L-glutamate
-
 ?
3.5.1.94 gamma-(glutamylamino)butanoate + H2O Cys114 is the active center of PuuD Escherichia coli K-12 gamma-aminobutanoate + L-glutamate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.94 dimer 2 * 28500, calcaulation from amino-acid sequence Escherichia coli K-12

Synonyms

EC Number Synonyms Comment Organism
3.5.1.94 PuuD
-
 Escherichia coli K-12

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.94 8.5 9
-
 Escherichia coli K-12