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Literature summary extracted from

  • Wu, X.B.; Fan, K.Q.; Wang, Q.H.; Yang, K.Q.
    C-terminus mutations of Acremonium chrysogenum deacetoxy/deacetylcephalosporin C synthase with improved activity toward penicillin analogs (2005), FEMS Microbiol. Lett., 246, 103-110.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.14.11.26 N305L improved ability to convert penicillin analogs in ring expansion reaction of EC 1.14.20.1 Acremonium chrysogenum
1.14.11.26 R308L improved ability to convert penicillin analogs in ring expansion reaction of EC 1.14.20.1 Acremonium chrysogenum
1.14.20.1 N305L improved ability to convert penicillin analogs in ring expansion reaction of EC 1.14.20.1 Acremonium chrysogenum
1.14.20.1 R307L activities comparable to wild-type Acremonium chrysogenum
1.14.20.1 R308L improved ability to convert penicillin analogs in ring expansion reaction of EC 1.14.20.1 Acremonium chrysogenum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.20.1 3.21
-
 penicillin G 30°C, mutant R308L Acremonium chrysogenum
1.14.20.1 4.51
-
 penicillin G 30°C, mutant N305L Acremonium chrysogenum
1.14.20.1 7.05
-
 penicillin G 30°C, mutant R307L Acremonium chrysogenum
1.14.20.1 7.1
-
 penicillin G 30°C, wild-type Acremonium chrysogenum

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.26 Acremonium chrysogenum
-
 catalyzes both reaction of EC 1.14.20.1 and of EC 1.14.11.26
-
1.14.20.1 Acremonium chrysogenum
-
 catalyzes both reaction of EC 1.14.20.1 and of EC 1.14.11.26
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.26 7-aminodeacetoxycephalosporanic acid + 2-oxoglutarate + O2 poor substrate Acremonium chrysogenum 7-aminodeacetylcephalosporin + succinate + CO2
-
 ?
1.14.20.1 amoxicillin + 2-oxoglutarate + O2 mutant R308L, 100% of activity, compared to wild-type Acremonium chrysogenum ? + succinate + CO2 + H2O
-
 ?
1.14.20.1 ampicillin + 2-oxoglutarate + O2 mutant N305L, 222%, mutant R307L, 100%, mutant R308L, 311% of activity, compared to wild-type Acremonium chrysogenum cephalexin + succinate + CO2 + H2O
-
 ?
1.14.20.1 carbenicillin + 2-oxoglutarate + O2 mutant N305L, 178%, mutant R307L, 93%, mutant R308L, 194% of activity, compared to wild-type Acremonium chrysogenum ? + succinate + CO2 + H2O
-
 ?
1.14.20.1 penicillin G + 2-oxoglutarate + O2 mutant N305L, 163%, mutant R307L, 100%, mutant R308L, 516% of activity, compared to wild-type Acremonium chrysogenum phenylacetyl-7-aminodeacetoxy cephalosporanic acid + succinate + CO2 + H2O
-
 ?
1.14.20.1 penicillin V + 2-oxoglutarate + O2 mutant N305L, 100%, mutant R307L, 93%, mutant R308L, 170% of activity, compared to wild-type Acremonium chrysogenum ? + succinate + CO2 + H2O
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.20.1 0.071
-
 penicillin G 30°C, mutant R307L Acremonium chrysogenum
1.14.20.1 0.074
-
 penicillin G 30°C, mutant N305L Acremonium chrysogenum
1.14.20.1 0.077
-
 penicillin G 30°C, wild-type Acremonium chrysogenum
1.14.20.1 0.128
-
 penicillin G 30°C, mutant R308L Acremonium chrysogenum