EC Number | Cloned (Comment) | Organism |
---|---|---|
6.5.1.6 | expressed in Escherichia coli BL21(DE3) pLysS | Thermococcus fumicolans |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
6.5.1.6 | Mg2+ | while optimal activity is obtained with 2 mM, it is almost totally inhibited with 10 mM MgCl2 | Thermococcus fumicolans |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.5.1.6 | 0.69 | - |
NAD+ | pH 6.5, 55°C | Thermococcus fumicolans | |
6.5.1.6 | 1.16 | - |
ATP | pH 6.5, 55°C | Thermococcus fumicolans |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
6.5.1.6 | K+ | the enzyme does not require monovalent cations to be active. 2 mM KCl or up to 5 mM of NaCl increase its activity up to 70%. Higher concentrations of these cations are inhibitory, but the enzyme still retains 50% activity with 100 mM NaCl or 100 mM KCl | Thermococcus fumicolans | |
6.5.1.6 | Mg2+ | strongly dependent on the MgCl2. While optimal activity is obtained with 2 mM, it is almost totally inhibited with 10 mM MgCl2 | Thermococcus fumicolans | |
6.5.1.6 | Na+ | the enzyme does not require monovalent cations to be active. 2 mM KCl or up to 5 mM of NaCl increase its activity up to 70%. Higher concentrations of these cations are inhibitory, but the enzyme still retains 50% activity with 100 mM NaCl or 100 mM KCl | Thermococcus fumicolans |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.5.1.6 | Thermococcus fumicolans | Q9HH07 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.5.1.6 | - |
Thermococcus fumicolans |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
6.5.1.6 | ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide | (2) | Thermococcus fumicolans | |
6.5.1.6 | ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + diphosphate | (1) | Thermococcus fumicolans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.5.1.6 | ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | the enzyme displays nick joining and blunt-end ligation activity using either ATP or NAD+, as a cofactor | Thermococcus fumicolans | AMP + diphosphate + (deoxyribonucleotide)n+m | - |
? | |
6.5.1.6 | NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | the enzyme displays nick joining and blunt-end ligation activity using either ATP or NAD+, as a cofactor | Thermococcus fumicolans | AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.5.1.6 | Tfu DNA ligase | - |
Thermococcus fumicolans |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.5.1.6 | 65 | - |
- |
Thermococcus fumicolans |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.5.1.6 | 40 | 80 | 40°C: 50% of maximal activity, 80°C: about 50% of maximal activity | Thermococcus fumicolans |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.5.1.6 | 80 | - |
3 h, stable | Thermococcus fumicolans |
6.5.1.6 | 90 | - |
15 min, 50% loss of activity | Thermococcus fumicolans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.5.1.6 | 7 | - |
- |
Thermococcus fumicolans |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
6.5.1.6 | 6.5 | - |
about 80% of maximal activity, pH 7.0: optimum, pH 7.5: about 10% of maximal activity | Thermococcus fumicolans |