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Literature summary extracted from

  • Murakami, S.; Kohsaka, C.; Okuno, T.; Takenaka, S.; Aoki, K.
    Purification, characterization, and gene cloning of cis,cis-muconate cycloisomerase from benzamide-assimilating Arthrobacter species BA-5-17 (2004), FEMS Microbiol. Lett., 231, 119-124.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.5.1.1 gene catB Arthrobacter sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.5.1.1 0.029
-
 2-methyl-cis,cis-muconate 24°C, pH 8.0 Arthrobacter sp.
5.5.1.1 0.046
-
 3-chloro-cis,cis-muconate 24°C, pH 8.0 Arthrobacter sp.
5.5.1.1 0.075
-
 3-methyl-cis,cis-muconate 24°C, pH 8.0 Arthrobacter sp.
5.5.1.1 0.15
-
 cis,cis-muconate 24°C, pH 8.0 Arthrobacter sp.
5.5.1.1 0.67
-
 3-ethyl-cis,cis-muconate 24°C, pH 8.0 Arthrobacter sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.5.1.1 Mg2+ 56% of the activity with Mn2+ Arthrobacter sp.
5.5.1.1 Mn2+ required, 25% residual activity without addition of Mn2+ Arthrobacter sp.
5.5.1.1 additional information Co2+ may not replace for Mn2+ Arthrobacter sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.5.1.1 40926
-
 6 * 43000, SDS-PAGE, 6 * 40926, calculated Arthrobacter sp.
5.5.1.1 43000
-
 6 * 43000, SDS-PAGE, 6 * 40926, calculated Arthrobacter sp.
5.5.1.1 280000
-
 gel filtration Arthrobacter sp.

Organism

EC Number Organism UniProt Comment Textmining
5.5.1.1 Arthrobacter sp.
-
-
-
5.5.1.1 Arthrobacter sp. BA-5-17
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.5.1.1
-
 Arthrobacter sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.5.1.1 41
-
 24°C, pH 8.0 Arthrobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.5.1.1 2-methyl-cis,cis-muconate
-
 Arthrobacter sp. 2-methylmuconolactone
-
 ?
5.5.1.1 3-Chloro-cis,cis-muconate
-
 Arthrobacter sp. 5-Chloromuconolactone
-
 ?
5.5.1.1 3-Chloro-cis,cis-muconate
-
 Arthrobacter sp. BA-5-17 5-Chloromuconolactone
-
 ?
5.5.1.1 3-ethyl-cis,cis-muconate
-
 Arthrobacter sp. 4-ethylmuconolactone
-
 ?
5.5.1.1 3-ethyl-cis,cis-muconate
-
 Arthrobacter sp. BA-5-17 4-ethylmuconolactone
-
 ?
5.5.1.1 3-methyl-cis,cis-muconate
-
 Arthrobacter sp. 4-methylmuconolactone
-
 ?
5.5.1.1 3-methyl-cis,cis-muconate
-
 Arthrobacter sp. BA-5-17 4-methylmuconolactone
-
 ?
5.5.1.1 cis,cis-Muconate
-
 Arthrobacter sp. (+)-Muconolactone
-
 ?
5.5.1.1 cis,cis-Muconate
-
 Arthrobacter sp. BA-5-17 (+)-Muconolactone
-
 ?
5.5.1.1 additional information no substrate: 2-chloro-cis,cis-muconate, 2-fluoro-cis,cis-muconate Arthrobacter sp. ?
-
 ?
5.5.1.1 additional information no substrate: 2-chloro-cis,cis-muconate, 2-fluoro-cis,cis-muconate Arthrobacter sp. BA-5-17 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
5.5.1.1 hexamer 6 * 43000, SDS-PAGE, 6 * 40926, calculated Arthrobacter sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.5.1.1 65
-
 10 min, more than 90% residual activity Arthrobacter sp.
5.5.1.1 70
-
 10 min, complete loss of activity Arthrobacter sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5.1.1 7.5
-
-
 Arthrobacter sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.5.1.1 7 11 more than 70% of maximum activity within this range Arthrobacter sp.