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Literature summary extracted from

  • Hansen, T.; Schonheit, P.
    ATP-dependent glucokinase from the hyperthermophilic bacterium Thermotoga maritima represents an extremely thermophilic ROK glucokinase with high substrate specificity (2003), FEMS Microbiol. Lett., 226, 405-411.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.2 gene TM 1469 or glk, functional expression of the soluble His-tagged in Escherichia coli strain BL21(DE3) Thermotoga maritima

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.2 Cu2+
-
 Thermotoga maritima
2.7.1.2 Fe2+
-
 Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.2 additional information
-
 additional information activation energy, kinetics Thermotoga maritima
2.7.1.2 0.36
-
 ATP recombinant enyme, pH 7.3, 80°C Thermotoga maritima
2.7.1.2 1
-
 D-glucose recombinant enyme, pH 7.3, 80°C Thermotoga maritima

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.2 Co2+ 50% of the activity with Mg2+ Thermotoga maritima
2.7.1.2 Mg2+ most efficient divalent cation Thermotoga maritima
2.7.1.2 Mn2+ 90% of the activity with Mg2+ Thermotoga maritima
2.7.1.2 Ni2+ 20% of the activity with Mg2+ Thermotoga maritima

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.2 36000
-
 2 * 36000, alpha2, recombinant enzyme, SDS-PAGE Thermotoga maritima
2.7.1.2 75000
-
 recombinant enzyme, gel filtration Thermotoga maritima

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.2 ATP + D-glucose Thermotoga maritima the enzyme catalyzes the first step of the Embden-Meyerhoff pathway and the Entner-Douderoff pathway ADP + D-glucose 6-phosphate
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.2 Thermotoga maritima
-
 gene TM 1469 or glk
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.2 recombinant soluble His-tagged enzyme from Escherichia coli strain BL21(DE3) by ultracentrifugation, nickel affinity chromatography, and gel filtration to homogeneity Thermotoga maritima

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.2 370
-
 purified recombinant enzyme, Vmax Thermotoga maritima

Storage Stability

EC Number Storage Stability Organism
2.7.1.2 -20°C, purified recombinant enzyme, 50 mM Tris-HCl, pH 7.5, stable Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.2 ATP + 2-deoxy-D-glucose 26% of the activity with D-glucose Thermotoga maritima ADP + 2-deoxy-D-glucose 6-phosphate
-
 ir
2.7.1.2 ATP + D-glucose the enzyme catalyzes the first step of the Embden-Meyerhoff pathway and the Entner-Douderoff pathway Thermotoga maritima ADP + D-glucose 6-phosphate
-
 ir
2.7.1.2 ATP + D-glucose the enzyme is specific for D-glucose Thermotoga maritima ADP + D-glucose 6-phosphate
-
 ir
2.7.1.2 CTP + D-glucose 5% of the activity with ATP as phosphoryl donor Thermotoga maritima CDP + D-glucose 6-phosphate
-
 ?
2.7.1.2 GTP + D-glucose 12% of the activity with ATP as phosphoryl donor Thermotoga maritima GDP + D-glucose 6-phosphate
-
 ?
2.7.1.2 ITP + D-glucose 20% of the activity with ATP as phosphoryl donor Thermotoga maritima IDP + D-glucose 6-phosphate
-
 ?
2.7.1.2 additional information no activity with D-mannose and D-fructose as phosphoryl acceptors, no activity with UDP, ADP, acetyl phosphate, and diphosphate as phosphoryl donors Thermotoga maritima ?
-
 ?
2.7.1.2 UTP + D-glucose 12% of the activity with ATP as phosphoryl donor Thermotoga maritima UDP + D-glucose 6-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.2 dimer 2 * 36000, alpha2, recombinant enzyme, SDS-PAGE Thermotoga maritima

Synonyms

EC Number Synonyms Comment Organism
2.7.1.2 ATP-GLK
-
 Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.2 93
-
-
 Thermotoga maritima

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.1.2 38 98 below 10% of maximal activity at 38°C, about 90% of maximal activity at 98°C Thermotoga maritima

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.2 80
-
 quite stable for 120 min Thermotoga maritima
2.7.1.2 100
-
 almost complete inactivation after 5 min Thermotoga maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.2 7.3
-
-
 Thermotoga maritima

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.2 6.8 7.7 50% activity at pH 6.8 and pH 7.7 Thermotoga maritima

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.2 ATP dependent on Thermotoga maritima
2.7.1.2 CTP 5% of the activity with ATP Thermotoga maritima
2.7.1.2 GTP 12% of the activity with ATP Thermotoga maritima
2.7.1.2 ITP 20% of the activity with ATP Thermotoga maritima
2.7.1.2 UTP 12% of the activity with ATP Thermotoga maritima