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Literature summary extracted from

  • Dufe, V.T.; Luersen, K.; Eschbach, M.L.; Haider, N.; Karlberg, T.; Walter, R.D.; Al-Karadaghi, S.
    Cloning, expression, characterisation and three-dimensional structure determination of Caenorhabditis elegans spermidine synthase (2005), FEBS Lett., 579, 6037-6043.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.16 expression in Escherichia coli Caenorhabditis elegans

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.16
-
 Caenorhabditis elegans

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.16 Cyclohexylamine IC50 2.4 +/- 0.7 microM Caenorhabditis elegans
2.5.1.16 trans-4-Methylcyclohexylamine IC50 7.2 microM Caenorhabditis elegans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.16 0.111
-
 S-adenosylmethioninamine
-
 Caenorhabditis elegans
2.5.1.16 0.158
-
 putrescine
-
 Caenorhabditis elegans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.16 35000
-
 calculated Caenorhabditis elegans
2.5.1.16 38000
-
 SDS-PAGE, including His-tag of 3 kDA Caenorhabditis elegans

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.16 Caenorhabditis elegans
-
-
-
2.5.1.16 Caenorhabditis elegans Bristol N2
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.16
-
 Caenorhabditis elegans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.16 1.8
-
-
 Caenorhabditis elegans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.16 putrescine + S-adenosylmethioninamine
-
 Caenorhabditis elegans spermidine + 5'-methylthioadenosine
-
 ?
2.5.1.16 putrescine + S-adenosylmethioninamine
-
 Caenorhabditis elegans Bristol N2 spermidine + 5'-methylthioadenosine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.16 dimer gel filtration, molecular weight of 78000 Caenorhabditis elegans

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.5.1.16 0.0024
-
 IC50 2.4 +/- 0.7 microM Caenorhabditis elegans Cyclohexylamine
2.5.1.16 0.0072
-
 IC50 7.2 microM Caenorhabditis elegans trans-4-Methylcyclohexylamine