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Literature summary extracted from

  • Ambrogelly, A.; Kamtekar, S.; Stathopoulos, C.; Kennedy, D.; Soll, D.
    Asymmetric behavior of archaeal prolyl-tRNA synthetase (2005), FEBS Lett., 579, 6017-6022.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.15 additional information C-terminal deletion mutant lacking last 50 amino acids, little effect on kinetic parameters Methanocaldococcus jannaschii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.15 0.002
-
 tRNAPro pH 7.0, 55°C, C-terminal deletion mutant Methanocaldococcus jannaschii
6.1.1.15 0.0022
-
 tRNAPro pH 7.0, 55°C, wild-type Methanocaldococcus jannaschii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.15 53300
-
 2 * 53300, calcultaed Methanocaldococcus jannaschii
6.1.1.15 55700
-
 2 * 55700, calculated Methanothermobacter thermautotrophicus
6.1.1.15 105000
-
 gel filtration Methanocaldococcus jannaschii
6.1.1.15 113000
-
 gel filtration Methanothermobacter thermautotrophicus

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.15 Methanocaldococcus jannaschii
-
-
-
6.1.1.15 Methanothermobacter thermautotrophicus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
6.1.1.15 ATP + L-proline + tRNAPro = AMP + diphosphate + L-prolyl-tRNAPro one tRNA binds per dimeric enzyme, only 50% of active sites are active Methanothermobacter thermautotrophicus
a
6.1.1.15 ATP + L-proline + tRNAPro = AMP + diphosphate + L-prolyl-tRNAPro one tRNA binds per dimeric enzyme, only 50% of active sites are active Methanocaldococcus jannaschii
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.15 ATP + L-proline + tRNAPro
-
 Methanothermobacter thermautotrophicus AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
6.1.1.15 ATP + L-proline + tRNAPro
-
 Methanocaldococcus jannaschii AMP + diphosphate + L-prolyl-tRNAPro
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.15 dimer 2 * 53300, calcultaed Methanocaldococcus jannaschii
6.1.1.15 dimer 2 * 55700, calculated Methanothermobacter thermautotrophicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.15 0.4
-
 tRNAPro pH 7.0, 55°C, C-terminal deletion mutant Methanocaldococcus jannaschii
6.1.1.15 1.6
-
 tRNAPro pH 7.0, 55°C, wild-type Methanocaldococcus jannaschii