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Literature summary extracted from

  • Langer, T.; Sreeramulu, S.; Vogtherr, M.; Elshorst, B.; Betz, M.; Schieborr, U.; Saxena, K.; Schwalbe, H.
    Folding and activity of cAMP-dependent protein kinase mutants (2005), FEBS Lett., 579, 4049-4054.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.11 cAMP dependent on Cricetulus griseus

Application

EC Number Application Comment Organism
2.7.11.11 drug development recombinant PKA can be mutated to resemble Akt/PKB, which cannot be easily recombinantly expressed, mutant PKA can be used as surrogate enzyme for Akt/PKB in drug design Cricetulus griseus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.11 expression of wild-type and mutant PKAs in Escherichia coli strain BL21(DE3) Cricetulus griseus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.11 additional information removal of residues S10, S139, and S338 plus Akt/PKB-like mutations of the ATP binding site render PKA to a Akt/PKB similar enzyme, overview Cricetulus griseus
2.7.11.11 S10A/S139D/S338D site-directed mutagenesis, mutant shows activity and properties similar to the wild-type enzyme Cricetulus griseus
2.7.11.11 S10A/S139D/T197D/S338D site-directed mutagenesis, mutant shows reduced expression level in Escherichia coli and impaired folding Cricetulus griseus
2.7.11.11 S10A/S139D/T197E/S338D site-directed mutagenesis, mutant shows reduced expression level in Escherichia coli and impaired folding Cricetulus griseus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.11 Mg2+
-
 Cricetulus griseus

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.11 Cricetulus griseus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.11.11 phosphoprotein autophosphorylation at Ser10, Ser139, Thr197, and Ser338 Cricetulus griseus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.11 recombinant wild-type and mutant PKAs from Escherichia coli strain BL21(DE3) Cricetulus griseus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.11 CHO cell
-
 Cricetulus griseus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.11 ATP + Kemptide activity of catalytic PKA subunit Cricetulus griseus ADP + phosphorylated Kemptide
-
 ?
2.7.11.11 additional information the enzyme performs autophosphorylation at Ser10, Ser139, Thr197, and Ser338 Cricetulus griseus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.11 More structure of catalytic subunit C with ATP binding cleft and activation loop, overview Cricetulus griseus

Synonyms

EC Number Synonyms Comment Organism
2.7.11.11 PKA
-
 Cricetulus griseus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.11 22
-
 assay at room temperature Cricetulus griseus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.11 7.5
-
 assay at Cricetulus griseus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.11 ATP
-
 Cricetulus griseus