BRENDA - Enzyme Database

Characterization and comparative analysis of Arabidopsis phosphatidylinositol phosphate 5-kinase 10 reveals differences in Arabidopsis and human phosphatidylinositol phosphate kinases

Perera, I.Y.; Davis, A.J.; Galanopoulou, D.; Im, Y.J.; Boss, W.F.; FEBS Lett. 579, 3427-3432 (2005)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.68
additional information
enzyme form 10 is insensitive to phosphatidic acid
Arabidopsis thaliana
2.7.1.68
phosphatidic acid
activates enzyme form 1 by 2.1fold
Arabidopsis thaliana
2.7.1.68
phosphatidic acid
activates isozyme alpha by 2.0fold
Homo sapiens
Cloned(Commentary)
EC Number
Commentary
Organism
2.7.1.68
expression of enzyme form 10 as GST-fusion protein in Escherichia coli, expression of His-tagged enzyme form 10 in Spodoptera frugiperda Sf9 cells via baculovirus infection system; expression of enzyme form 1 as GST-fusion protein in Escherichia coli, expression of His-tagged enzyme form 1 in Spodoptera frugiperda Sf9 cells via baculovirus infection system
Arabidopsis thaliana
2.7.1.68
expression of isozyme alpha as GST-fusion protein in Escherichia coli
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.68
0.065
-
1-phosphatidyl-1D-myo-inositol 4-phosphate
recombinant enzyme form 10
Arabidopsis thaliana
2.7.1.68
0.6
-
1-phosphatidyl-1D-myo-inositol 4-phosphate
recombinant enzyme form 1
Arabidopsis thaliana
2.7.1.68
4.1
-
1-phosphatidyl-1D-myo-inositol 4-phosphate
recombinant isozyme alpha
Homo sapiens
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.68
Mg2+
;
Arabidopsis thaliana
2.7.1.68
Mg2+
-
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.68
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
Arabidopsis thaliana
-
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
-
?
2.7.1.68
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
Homo sapiens
-
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.68
Arabidopsis thaliana
Q56YP2
enzyme form 1; enzyme form 1
-
2.7.1.68
Arabidopsis thaliana
Q9LMN1
enzyme form 10; enzyme form 10
-
2.7.1.68
Homo sapiens
Q99755
isozyme alpha type I; type I isozyme alpha
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.68
recombinan GST-fusion isozyme alpha from Escherichia coli
Homo sapiens
2.7.1.68
recombinant GST-fusion protein from Escherichia coli in presence of Triton X-100 and PtdOH; recombinant GST-fusion protein from Escherichia coli in presence of Triton X-100 and PtdOH
Arabidopsis thaliana
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.68
flower
enzyme form 10, most abundant in
Arabidopsis thaliana
-
2.7.1.68
inflorescence stalk
enzyme form 10, most abundant in
Arabidopsis thaliana
-
2.7.1.68
additional information
tissue distribution; tissue distribution, enzyme form 1 is present in all tissues
Arabidopsis thaliana
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.7.1.68
additional information
-
enzyme form 10 shows 10fold lower activity than enzyme form 1 in recombinant Sf9 cells, overview
Arabidopsis thaliana
2.7.1.68
additional information
-
recombinant isozyme alpha
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.68
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
661757
Arabidopsis thaliana
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
-
-
?
2.7.1.68
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
661757
Homo sapiens
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.68
ATP
;
Arabidopsis thaliana
2.7.1.68
ATP
-
Homo sapiens
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.68
additional information
enzyme form 10 is insensitive to phosphatidic acid
Arabidopsis thaliana
2.7.1.68
phosphatidic acid
activates enzyme form 1 by 2.1fold
Arabidopsis thaliana
2.7.1.68
phosphatidic acid
activates isozyme alpha by 2.0fold
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.68
expression of enzyme form 1 as GST-fusion protein in Escherichia coli, expression of His-tagged enzyme form 1 in Spodoptera frugiperda Sf9 cells via baculovirus infection system
Arabidopsis thaliana
2.7.1.68
expression of enzyme form 10 as GST-fusion protein in Escherichia coli, expression of His-tagged enzyme form 10 in Spodoptera frugiperda Sf9 cells via baculovirus infection system
Arabidopsis thaliana
2.7.1.68
expression of isozyme alpha as GST-fusion protein in Escherichia coli
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.68
ATP
-
Arabidopsis thaliana
2.7.1.68
ATP
-
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.68
0.065
-
1-phosphatidyl-1D-myo-inositol 4-phosphate
recombinant enzyme form 10
Arabidopsis thaliana
2.7.1.68
0.6
-
1-phosphatidyl-1D-myo-inositol 4-phosphate
recombinant enzyme form 1
Arabidopsis thaliana
2.7.1.68
4.1
-
1-phosphatidyl-1D-myo-inositol 4-phosphate
recombinant isozyme alpha
Homo sapiens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.68
Mg2+
-
Arabidopsis thaliana
2.7.1.68
Mg2+
-
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.68
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
Arabidopsis thaliana
-
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
-
?
2.7.1.68
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
Homo sapiens
-
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.68
recombinan GST-fusion isozyme alpha from Escherichia coli
Homo sapiens
2.7.1.68
recombinant GST-fusion protein from Escherichia coli in presence of Triton X-100 and PtdOH
Arabidopsis thaliana
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.68
flower
enzyme form 10, most abundant in
Arabidopsis thaliana
-
2.7.1.68
inflorescence stalk
enzyme form 10, most abundant in
Arabidopsis thaliana
-
2.7.1.68
additional information
tissue distribution
Arabidopsis thaliana
-
2.7.1.68
additional information
tissue distribution, enzyme form 1 is present in all tissues
Arabidopsis thaliana
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.7.1.68
additional information
-
enzyme form 10 shows 10fold lower activity than enzyme form 1 in recombinant Sf9 cells, overview
Arabidopsis thaliana
2.7.1.68
additional information
-
recombinant isozyme alpha
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.68
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
661757
Arabidopsis thaliana
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
-
-
?
2.7.1.68
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
661757
Homo sapiens
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
-
-
?