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Literature summary extracted from

  • Perera, I.Y.; Davis, A.J.; Galanopoulou, D.; Im, Y.J.; Boss, W.F.
    Characterization and comparative analysis of Arabidopsis phosphatidylinositol phosphate 5-kinase 10 reveals differences in Arabidopsis and human phosphatidylinositol phosphate kinases (2005), FEBS Lett., 579, 3427-3432.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.68 additional information enzyme form 10 is insensitive to phosphatidic acid Arabidopsis thaliana
2.7.1.68 phosphatidic acid activates enzyme form 1 by 2.1fold Arabidopsis thaliana
2.7.1.68 phosphatidic acid activates isozyme alpha by 2.0fold Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.68 expression of enzyme form 1 as GST-fusion protein in Escherichia coli, expression of His-tagged enzyme form 1 in Spodoptera frugiperda Sf9 cells via baculovirus infection system Arabidopsis thaliana
2.7.1.68 expression of enzyme form 10 as GST-fusion protein in Escherichia coli, expression of His-tagged enzyme form 10 in Spodoptera frugiperda Sf9 cells via baculovirus infection system Arabidopsis thaliana
2.7.1.68 expression of isozyme alpha as GST-fusion protein in Escherichia coli Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.68 0.065
-
 1-phosphatidyl-1D-myo-inositol 4-phosphate recombinant enzyme form 10 Arabidopsis thaliana
2.7.1.68 0.6
-
 1-phosphatidyl-1D-myo-inositol 4-phosphate recombinant enzyme form 1 Arabidopsis thaliana
2.7.1.68 4.1
-
 1-phosphatidyl-1D-myo-inositol 4-phosphate recombinant isozyme alpha Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.68 Mg2+
-
 Arabidopsis thaliana
2.7.1.68 Mg2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.68 ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate Arabidopsis thaliana
-
 ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
 ?
2.7.1.68 ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate Homo sapiens
-
 ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.68 Arabidopsis thaliana Q56YP2 enzyme form 1; enzyme form 1
-
2.7.1.68 Arabidopsis thaliana Q9LMN1 enzyme form 10; enzyme form 10
-
2.7.1.68 Homo sapiens Q99755 isozyme alpha type I; type I isozyme alpha
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.68 recombinan GST-fusion isozyme alpha from Escherichia coli Homo sapiens
2.7.1.68 recombinant GST-fusion protein from Escherichia coli in presence of Triton X-100 and PtdOH Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.68 flower enzyme form 10, most abundant in Arabidopsis thaliana
-
2.7.1.68 inflorescence stalk enzyme form 10, most abundant in Arabidopsis thaliana
-
2.7.1.68 additional information tissue distribution Arabidopsis thaliana
-
2.7.1.68 additional information tissue distribution, enzyme form 1 is present in all tissues Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.68 additional information
-
 enzyme form 10 shows 10fold lower activity than enzyme form 1 in recombinant Sf9 cells, overview Arabidopsis thaliana
2.7.1.68 additional information
-
 recombinant isozyme alpha Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.68 ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
 Arabidopsis thaliana ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
 ?
2.7.1.68 ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
 Homo sapiens ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.68 More phosphatidylinositol phosphate 5-kinase 1 belongs to the subfamily B Arabidopsis thaliana
2.7.1.68 More phosphatidylinositol phosphate 5-kinase 10 belongs to the subfamily A Arabidopsis thaliana
2.7.1.68 phosphatidylinositol phosphate 5-kinase 1
-
 Arabidopsis thaliana
2.7.1.68 phosphatidylinositol phosphate 5-kinase 10
-
 Arabidopsis thaliana
2.7.1.68 PIPK1
-
 Arabidopsis thaliana
2.7.1.68 PIPK10
-
 Arabidopsis thaliana
2.7.1.68 PIPKIalpha
-
 Homo sapiens
2.7.1.68 PtdInsP
-
 Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.68 ATP
-
 Arabidopsis thaliana
2.7.1.68 ATP
-
 Homo sapiens