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Literature summary extracted from

  • Yokota, S.; Taniguchi, Y.; Kihara, A.; Mitsutake, S.; Igarashi, Y.
    Asp177 in C4 domain of mouse sphingosine kinase 1a is important for the sphingosine recognition (2004), FEBS Lett., 578, 106-110.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.91 Calmodulin Ca2+-dependent binding of recombinant wild-type and mutant enzymes Mus musculus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.91 expression of FLAG-tagged isozyme SK1 splicing variant a in HEK293 cells Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.91 D175N site-directed mutagenesis, about 50% of wild-type enzyme activity Mus musculus
2.7.1.91 D175N/D177N site-directed mutagenesis, less than 5% of wild-type enzyme activity Mus musculus
2.7.1.91 D177N site-directed mutagenesis, less than 10% of wild-type enzyme activity Mus musculus
2.7.1.91 D177N/E179Q site-directed mutagenesis, nearly inactive mutant Mus musculus
2.7.1.91 E179Q site-directed mutagenesis, about 30% of wild-type enzyme activity Mus musculus
2.7.1.91 E181Q site-directed mutagenesis, about 30% of wild-type enzyme activity Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.91 additional information
-
 additional information kinetics, recombinant wild-type and mutant enzymes Mus musculus
2.7.1.91 0.01
-
 sphingosine pH 7.4, 37°C, recombinant wild-type enzyme and mutant E181Q Mus musculus
2.7.1.91 0.026
-
 sphingosine pH 7.4, 37°C, recombinant mutant E179Q Mus musculus
2.7.1.91 0.037
-
 sphingosine pH 7.4, 37°C, recombinant mutant D175N Mus musculus
2.7.1.91 0.063
-
 ATP pH 7.4, 37°C, recombinant mutant E179Q Mus musculus
2.7.1.91 0.072
-
 ATP pH 7.4, 37°C, recombinant wild-type enzyme Mus musculus
2.7.1.91 0.077
-
 ATP pH 7.4, 37°C, recombinant mutant D175N Mus musculus
2.7.1.91 0.088
-
 ATP pH 7.4, 37°C, recombinant mutant D177N Mus musculus
2.7.1.91 0.095
-
 ATP pH 7.4, 37°C, recombinant mutant E181Q Mus musculus
2.7.1.91 0.108
-
 sphingosine pH 7.4, 37°C, recombinant mutant D177N Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.91 Ca2+ Ca2+-dependent calmodulin binding of recombinant wild-type and mutant enzymes Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.91 ATP + sphingosine Mus musculus involved in diverse biological functions ADP + sphingosine 1-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.91 Mus musculus Q8CI15 isozyme SK1 splicing variant a
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.91 ATP + a sphingoid base = ADP + a sphingoid base 1-phosphate Asp177 in C4 domain of isozyme SK1a is important for sphingosine substrate recognition Mus musculus
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.91 additional information
-
 Vmax of recombinant wild-type and mutant enzymes Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.91 ATP + sphingosine
-
 Mus musculus ADP + sphingosine 1-phosphate
-
 ?
2.7.1.91 ATP + sphingosine involved in diverse biological functions Mus musculus ADP + sphingosine 1-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.91 SK
-
 Mus musculus
2.7.1.91 sphinganine kinase
-
 Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.91 37
-
 assay at Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.91 7.4
-
 assay at Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.91 ATP
-
 Mus musculus