Literature summary extracted from
Leboulle, G.; Muller, U.
Synergistic activation of insect cAMP-dependent protein kinase A (type II) by cyclicAMP and cyclicGMP (2004), FEBS Lett., 576, 216-220.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
2.7.11.11 |
cAMP |
dependent on, acts synergistically with cGMP, binding site domain structure on the regulatory subunit RII |
Apis mellifera |
|
2.7.11.11 |
cGMP |
activates, acts synergistically with cAMP |
Apis mellifera |
|
2.7.11.11 |
additional information |
phosphate-binding cassettes PCB of domain A and B of the regulatory subunits |
Apis mellifera |
|
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.7.11.11 |
regulatory subunit RII, DNA sequence determination and analysis, phylogenetic analysis |
Apis mellifera |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
2.7.11.11 |
Mg2+ |
- |
Apis mellifera |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.7.11.11 |
Apis mellifera |
Q6ZXJ1 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
2.7.11.11 |
PKAII holoenzyme from bee brain by ion exchange, adsorption, and cAMP-affinity chromatography, thereby dissociation of regulatory and catalytic subunits, and reconstitution |
Apis mellifera |
Renatured (Commentary)
EC Number |
Renatured (Comment) |
Organism |
---|
2.7.11.11 |
reconstitution of holoenzyme from purified subunits in 50 mM Tris-HCl, pH 7.8, 1 mM EDTA, 1 mM EGTA, 10 mM 2-mercaptoethanol, and 0.1 M NaCl |
Apis mellifera |
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
2.7.11.11 |
brain |
- |
Apis mellifera |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.7.11.11 |
ATP + phosphatase inhibitor PKI |
- |
Apis mellifera |
ADP + phosphorylated phosphatase inhibitor PKI |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
2.7.11.11 |
More |
structure, deduced from DNA and amino acid sequence, analysis and comparison |
Apis mellifera |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
2.7.11.11 |
PKAII |
- |
Apis mellifera |
2.7.11.11 |
protein kinase A type II |
- |
Apis mellifera |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
2.7.11.11 |
20 |
- |
assay at |
Apis mellifera |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
2.7.11.11 |
7.5 |
- |
assay at |
Apis mellifera |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
2.7.11.11 |
ATP |
- |
Apis mellifera |
|