Animal deoxyribonucleoside kinases: 'forward' and 'retrograde' evolution of their substrate specificity
Piskur, J.; Sandrini, M.P.B.; Knecht, W.; Munch-Petersen, B.; FEBS Lett. 560, 3-6 (2004) 
Data extracted from this reference:
Engineering
EC Number
Protein Variants
Commentary
Organism
2.7.1.74
A100V/R104M/D133A
site-directed mutagenesis, mutations alter the residues to the multisubstrate nucleoside kinase, EC 2.7.1.145, mutant shows 30fold increased kcat for deoxycytidine compared to the wild-type enzyme
Homo sapiens
2.7.1.74
R104M/D133A
site-directed mutagenesis, mutations alter the residues to the multisubstrate nucleoside kinase, EC 2.7.1.145, the mutant gains the ability to phosphorylate deoxythymidine
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.74
cytosol
-
Mus musculus
5829
-
2.7.1.74
cytosol
-
Homo sapiens
5829
-
2.7.1.113
mitochondrion
-
Homo sapiens
5739
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.74
Mg2+
-
Mus musculus
2.7.1.74
Mg2+
-
Homo sapiens
2.7.1.113
Mg2+
-
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2.7.1.74
ATP + 2'-deoxyadenosine
Homo sapiens
-
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
?
2.7.1.74
ATP + 2'-deoxyadenosine
Mus musculus
-
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
?
2.7.1.74
ATP + 2'-deoxycytidine
Homo sapiens
-
ADP + 2'-deoxycytidine 5'-phosphate
-
-
?
2.7.1.74
ATP + 2'-deoxycytidine
Mus musculus
-
ADP + 2'-deoxycytidine 5'-phosphate
-
-
?
2.7.1.74
ATP + 2'-deoxyguanosine
Homo sapiens
-
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
?
2.7.1.74
ATP + 2'-deoxyguanosine
Mus musculus
-
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
?
2.7.1.74
additional information
Homo sapiens
the earliest nucleotide kinase in evolution, probably encoded by the so-called dCK/dGK/TK2-like gene, is the progenitor gene, several duplications of it are the reason for evolutionary occurrence of nucleoside kinases with strict substrate specificities in other organism, phylogenetic tree, overview
?
-
-
-
2.7.1.74
additional information
Mus musculus
the earliest nucleotide kinase in evolution, probably encoded by the so-called dCK/dGK/TK2-like gene, is the progenitor gene, several duplications of it are the reason for evolutionary occurrence of nucleoside kinases with strict substrate specificities in other organism, phylogenetic tree, overview
?
-
-
-
2.7.1.113
ATP + 2'-deoxyadenosine
Homo sapiens
-
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
?
2.7.1.113
ATP + 2'-deoxyguanosine
Homo sapiens
-
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
?
2.7.1.113
ATP + 2'-deoxyinosine
Homo sapiens
-
ADP + 2'-deoxyinosine 5'-phosphate
-
-
?
2.7.1.113
additional information
Homo sapiens
the earliest nucleotide kinase in evolution, probably encoded by the so-called dCK/dGK/TK2-like gene, is the progenitor gene, several duplications of it are the reason for evolutionary occurring of nucleoside kinases with strict substrate specificities in other organism, phylogenetic tree, overview
?
-
-
-
2.7.1.145
additional information
Drosophila melanogaster
enzyme shows broad substate specificity, this enzyme specificity-type is the earliest in evolution probably encoded by the so-called dCK/dGK/TK2-like gene, several duplications of the progenitor gene are the reason for evolutionary occurring of nucleoside kinases with strict substrate specificities in other organism, phylogenetic tree, insects lost all but 1 nucleotide kinase, overview
?
-
-
-
Organism
EC Number
Organism
UniProt
Commentary
Textmining
2.7.1.74
Homo sapiens
P27707
-
-
2.7.1.74
Mus musculus
P43346
-
-
2.7.1.113
Homo sapiens
Q16854
-
-
2.7.1.145
Drosophila melanogaster
Q9XZT6
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2.7.1.74
ATP + 2'-deoxyadenosine
-
661731
Homo sapiens
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + 2'-deoxyadenosine
-
661731
Mus musculus
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + 2'-deoxycytidine
-
661731
Homo sapiens
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + 2'-deoxycytidine
-
661731
Mus musculus
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + 2'-deoxyguanosine
-
661731
Homo sapiens
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + 2'-deoxyguanosine
-
661731
Mus musculus
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.74
additional information
the earliest nucleotide kinase in evolution, probably encoded by the so-called dCK/dGK/TK2-like gene, is the progenitor gene, several duplications of it are the reason for evolutionary occurrence of nucleoside kinases with strict substrate specificities in other organism, phylogenetic tree, overview
661731
Homo sapiens
?
-
-
-
-
2.7.1.74
additional information
the earliest nucleotide kinase in evolution, probably encoded by the so-called dCK/dGK/TK2-like gene, is the progenitor gene, several duplications of it are the reason for evolutionary occurrence of nucleoside kinases with strict substrate specificities in other organism, phylogenetic tree, overview
661731
Mus musculus
?
-
-
-
-
2.7.1.113
ATP + 2'-deoxyadenosine
-
661731
Homo sapiens
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.113
ATP + 2'-deoxyguanosine
-
661731
Homo sapiens
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.113
ATP + 2'-deoxyinosine
-
661731
Homo sapiens
ADP + 2'-deoxyinosine 5'-phosphate
-
-
-
?
2.7.1.113
additional information
the earliest nucleotide kinase in evolution, probably encoded by the so-called dCK/dGK/TK2-like gene, is the progenitor gene, several duplications of it are the reason for evolutionary occurring of nucleoside kinases with strict substrate specificities in other organism, phylogenetic tree, overview
661731
Homo sapiens
?
-
-
-
-
2.7.1.145
additional information
enzyme shows broad substate specificity, this enzyme specificity-type is the earliest in evolution probably encoded by the so-called dCK/dGK/TK2-like gene, several duplications of the progenitor gene are the reason for evolutionary occurring of nucleoside kinases with strict substrate specificities in other organism, phylogenetic tree, insects lost all but 1 nucleotide kinase, overview
661731
Drosophila melanogaster
?
-
-
-
-
2.7.1.145
additional information
enzyme shows broad substate specificity involving V84, M88, and A110
661731
Drosophila melanogaster
?
-
-
-
-
Synonyms
EC Number
Synonyms
Commentary
Organism
2.7.1.74
dCK
-
Mus musculus
2.7.1.74
dCK
-
Homo sapiens
2.7.1.113
DGK
-
Homo sapiens
2.7.1.145
dNK
-
Drosophila melanogaster
2.7.1.145
More
the enzyme belongs to the thymidine kinase 2-like, i.e. TK2-like, family of enzymes
Drosophila melanogaster
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.74
ATP
-
Homo sapiens
2.7.1.74
ATP
-
Mus musculus
2.7.1.113
ATP
-
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.74
ATP
-
Homo sapiens
2.7.1.74
ATP
-
Mus musculus
2.7.1.113
ATP
-
Homo sapiens
Engineering (protein specific)
EC Number
Protein Variants
Commentary
Organism
2.7.1.74
A100V/R104M/D133A
site-directed mutagenesis, mutations alter the residues to the multisubstrate nucleoside kinase, EC 2.7.1.145, mutant shows 30fold increased kcat for deoxycytidine compared to the wild-type enzyme
Homo sapiens
2.7.1.74
R104M/D133A
site-directed mutagenesis, mutations alter the residues to the multisubstrate nucleoside kinase, EC 2.7.1.145, the mutant gains the ability to phosphorylate deoxythymidine
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.74
cytosol
-
Homo sapiens
5829
-
2.7.1.74
cytosol
-
Mus musculus
5829
-
2.7.1.113
mitochondrion
-
Homo sapiens
5739
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.74
Mg2+
-
Homo sapiens
2.7.1.74
Mg2+
-
Mus musculus
2.7.1.113
Mg2+
-
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2.7.1.74
ATP + 2'-deoxyadenosine
Homo sapiens
-
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
?
2.7.1.74
ATP + 2'-deoxyadenosine
Mus musculus
-
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
?
2.7.1.74
ATP + 2'-deoxycytidine
Homo sapiens
-
ADP + 2'-deoxycytidine 5'-phosphate
-
-
?
2.7.1.74
ATP + 2'-deoxycytidine
Mus musculus
-
ADP + 2'-deoxycytidine 5'-phosphate
-
-
?
2.7.1.74
ATP + 2'-deoxyguanosine
Homo sapiens
-
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
?
2.7.1.74
ATP + 2'-deoxyguanosine
Mus musculus
-
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
?
2.7.1.74
additional information
Homo sapiens
the earliest nucleotide kinase in evolution, probably encoded by the so-called dCK/dGK/TK2-like gene, is the progenitor gene, several duplications of it are the reason for evolutionary occurrence of nucleoside kinases with strict substrate specificities in other organism, phylogenetic tree, overview
?
-
-
-
2.7.1.74
additional information
Mus musculus
the earliest nucleotide kinase in evolution, probably encoded by the so-called dCK/dGK/TK2-like gene, is the progenitor gene, several duplications of it are the reason for evolutionary occurrence of nucleoside kinases with strict substrate specificities in other organism, phylogenetic tree, overview
?
-
-
-
2.7.1.113
ATP + 2'-deoxyadenosine
Homo sapiens
-
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
?
2.7.1.113
ATP + 2'-deoxyguanosine
Homo sapiens
-
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
?
2.7.1.113
ATP + 2'-deoxyinosine
Homo sapiens
-
ADP + 2'-deoxyinosine 5'-phosphate
-
-
?
2.7.1.113
additional information
Homo sapiens
the earliest nucleotide kinase in evolution, probably encoded by the so-called dCK/dGK/TK2-like gene, is the progenitor gene, several duplications of it are the reason for evolutionary occurring of nucleoside kinases with strict substrate specificities in other organism, phylogenetic tree, overview
?
-
-
-
2.7.1.145
additional information
Drosophila melanogaster
enzyme shows broad substate specificity, this enzyme specificity-type is the earliest in evolution probably encoded by the so-called dCK/dGK/TK2-like gene, several duplications of the progenitor gene are the reason for evolutionary occurring of nucleoside kinases with strict substrate specificities in other organism, phylogenetic tree, insects lost all but 1 nucleotide kinase, overview
?
-
-
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2.7.1.74
ATP + 2'-deoxyadenosine
-
661731
Homo sapiens
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + 2'-deoxyadenosine
-
661731
Mus musculus
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + 2'-deoxycytidine
-
661731
Homo sapiens
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + 2'-deoxycytidine
-
661731
Mus musculus
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + 2'-deoxyguanosine
-
661731
Homo sapiens
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + 2'-deoxyguanosine
-
661731
Mus musculus
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.74
additional information
the earliest nucleotide kinase in evolution, probably encoded by the so-called dCK/dGK/TK2-like gene, is the progenitor gene, several duplications of it are the reason for evolutionary occurrence of nucleoside kinases with strict substrate specificities in other organism, phylogenetic tree, overview
661731
Homo sapiens
?
-
-
-
-
2.7.1.74
additional information
the earliest nucleotide kinase in evolution, probably encoded by the so-called dCK/dGK/TK2-like gene, is the progenitor gene, several duplications of it are the reason for evolutionary occurrence of nucleoside kinases with strict substrate specificities in other organism, phylogenetic tree, overview
661731
Mus musculus
?
-
-
-
-
2.7.1.113
ATP + 2'-deoxyadenosine
-
661731
Homo sapiens
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.113
ATP + 2'-deoxyguanosine
-
661731
Homo sapiens
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.113
ATP + 2'-deoxyinosine
-
661731
Homo sapiens
ADP + 2'-deoxyinosine 5'-phosphate
-
-
-
?
2.7.1.113
additional information
the earliest nucleotide kinase in evolution, probably encoded by the so-called dCK/dGK/TK2-like gene, is the progenitor gene, several duplications of it are the reason for evolutionary occurring of nucleoside kinases with strict substrate specificities in other organism, phylogenetic tree, overview
661731
Homo sapiens
?
-
-
-
-
2.7.1.145
additional information
enzyme shows broad substate specificity, this enzyme specificity-type is the earliest in evolution probably encoded by the so-called dCK/dGK/TK2-like gene, several duplications of the progenitor gene are the reason for evolutionary occurring of nucleoside kinases with strict substrate specificities in other organism, phylogenetic tree, insects lost all but 1 nucleotide kinase, overview
661731
Drosophila melanogaster
?
-
-
-
-
2.7.1.145
additional information
enzyme shows broad substate specificity involving V84, M88, and A110
661731
Drosophila melanogaster
?
-
-
-
-