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Literature summary extracted from

  • Geguchadze, R.; Zhi, G.; Lau, K.S.; Isotani, E.; Persechini, A.; Kamm, K.E.; Stull, J.T.
    Quantitative measurements of Ca(2+)/calmodulin binding and activation of myosin light chain kinase in cells (2004), FEBS Lett., 557, 121-124.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.18 stable expression of a constructed Ca2+/calmodulin-dependent fluorescent biosensor MLCK in HEK-293 cells Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.18 Ca2+ dependent on Homo sapiens
2.7.11.18 Mg2+
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.18 190000
-
 x * 190000, recombinant Ca2+/calmodulin-dependent fluorescent biosensor MLCK, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.18 ATP + [myosin II regulatory light chain] Homo sapiens involved in many cellular cytoskeletal functions ADP + [myosin II regulatory light chain] phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.18 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.18 smooth muscle
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.18 additional information
-
 quantification of enzyme activity using a Ca2+/calmodulin-dependent fluorescent biosensor MLCK in vitro or in vivo, overview Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.18 ATP + [myosin II regulatory light chain]
-
 Homo sapiens ADP + [myosin II regulatory light chain] phosphate
-
 ?
2.7.11.18 ATP + [myosin II regulatory light chain] involved in many cellular cytoskeletal functions Homo sapiens ADP + [myosin II regulatory light chain] phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.18 ? x * 190000, recombinant Ca2+/calmodulin-dependent fluorescent biosensor MLCK, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.7.11.18 MLCK
-
 Homo sapiens
2.7.11.18 myosine light chain kinase
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.18 7
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.18 ATP
-
 Homo sapiens
2.7.11.18 Calmodulin dependent on Homo sapiens