EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.11.16 | expression of isoforms mGRK6-A, mGRK6-B, mGRK-C, and mGRK-D in Spodoptera frugiperda Sf9 cells via baculovirus infection system, transient expression of isoforms mGRK6-A, mGRK6-B, mGRK-C, and mGRK-D in COS-7 cells, recombinant isoforms mGRK6-A, mGRK6-B, and mGRK-C are membrane-associated, recombinant isoform mGRK6-D is inactive and located in the nucleus | Mus musculus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.11.16 | additional information | construction of isozymes mGRK6-A, mGRK6-B, mGRK-C, and mGRK-D by alternative splicing, recombinant isoforms mGRK6-A, mGRK6-B, and mGRK-C are membrane-associated, recombinant isoform mGRK6-D is inactive and located in the nucleus, structure-activity relationships of the enzyme C-terminus, overview | Mus musculus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.11.16 | additional information | the variable C-terminal extension of GRK6 constitutes a domain with autoinhibitory function involving residues D560, S566, and L576 of 3 inhibitory elements and an intervening stimulatory element | Mus musculus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.11.16 | Mg2+ | - |
Mus musculus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.11.16 | ATP + [G-protein-coupled receptor] | Mus musculus | - |
ADP + [G-protein-coupled receptor]phosphate | - |
? | |
2.7.11.16 | additional information | Mus musculus | the variable C-terminal extension of GRK6 constitutes an accessorial autoregulatory domain | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.11.16 | Mus musculus | - |
4 isoforms mGRK6-A, mGRK6-B, mGRK-C, and mGRK-D by alternative splicing | - |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
2.7.11.16 | lipoprotein | the C-terminus of GRK6 is palmitoylated, not required for phosphorylation activity | Mus musculus |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.11.16 | recombinant isozymes mGRK6-A, mGRK6-B, and mGRK-C from Sf9 insect cells by sequential gel filtration and heparin affinity chromatography | Mus musculus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.11.16 | ATP + rhodopsin | light-activated rhodopsin, recombinant isoforms mGRK6-A, mGRK6-B, and mGRK-C, the latter showing highest activity | Mus musculus | ADP + phosphorylated rhodopsin | - |
? | |
2.7.11.16 | ATP + [G-protein-coupled receptor] | - |
Mus musculus | ADP + [G-protein-coupled receptor]phosphate | - |
? | |
2.7.11.16 | additional information | the variable C-terminal extension of GRK6 constitutes an accessorial autoregulatory domain | Mus musculus | ? | - |
? | |
2.7.11.16 | additional information | structure-activity relationships of the enzyme C-terminus, overview | Mus musculus | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.11.16 | More | the variable C-terminal extension of GRK6 constitutes an accessorial autoregulatory domain consisting of 3 autoregulatory elements involving residues D560, S566, and L576 and an intervening stimulatory element | Mus musculus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.11.16 | G-protein-coupled receptor kinase 6 | - |
Mus musculus |
2.7.11.16 | GRK6 | - |
Mus musculus |
2.7.11.16 | More | GRK6 belongs to the GRK4 subfamily of the GRK family | Mus musculus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.11.16 | 30 | - |
assay at | Mus musculus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.11.16 | 7.5 | - |
assay at | Mus musculus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.7.11.16 | ATP | - |
Mus musculus |