Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Maerker, C.; Rohde, M.; Brakhage, A.A.; Brock, M.
    Methylcitrate synthase from Aspergillus fumigatus. Propionyl-CoA affects polyketide synthesis, growth and morphology of conidia (2005), FEBS J., 272, 3615-3630.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.3.3.5 additional information construction of an enzyme deletion mutant strain, which is unable to grow on propionate as sole carbon source, phenotype and growth behaviour analysis, accumulation of propionyl-CoA, morphology of conidia, deletion leads to an attenuation of virulence, overview Aspergillus fumigatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.5 acetyl-CoA competitive to CoA Aspergillus fumigatus
2.3.3.5 propionyl-CoA competitive to CoA Aspergillus fumigatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.5 0.0019
-
 propanoyl-CoA pH 8.0, 25°C, purified native enzyme Aspergillus fumigatus
2.3.3.5 0.0026
-
 acetyl-CoA pH 8.0, 25°C, purified native enzyme Aspergillus fumigatus
2.3.3.5 0.0027
-
 oxaloacetate pH 8.0, 25°C, purified native enzyme Aspergillus fumigatus
2.3.3.5 0.0085
-
 CoA pH 8.0, 25°C, purified native enzyme, in absence of propionyl-CoA Aspergillus fumigatus
2.3.3.5 0.0325
-
 CoA pH 8.0, 25°C, purified native enzyme, in presence of propionyl-CoA Aspergillus fumigatus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.3.5 mitochondrion enzyme possesses a mitochondrial signal import sequence Aspergillus fumigatus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.5 45000
-
 x * 45000, native enzyme, SDS-PAGE, x * 514100, sequence calculation Aspergillus fumigatus
2.3.3.5 514100
-
 x * 45000, native enzyme, SDS-PAGE, x * 514100, sequence calculation Aspergillus fumigatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.3.5 additional information Aspergillus fumigatus key enzyme of the methylcitrate cycle and required for fungal propionate degradation ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.5 Aspergillus fumigatus Q50I20 opportunistic human pathogen, strain CEA17, strain ATCC 46654
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.5 native enzyme 136fold to homogeneity Aspergillus fumigatus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.3.5 mycelium
-
 Aspergillus fumigatus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.3.5 17.7
-
 purified native enzyme, with oxaloacetate and propionyl-CoA Aspergillus fumigatus
2.3.3.5 48
-
 purified native enzyme, with oxaloacetate and acetyl-CoA Aspergillus fumigatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.5 acetyl-CoA + H2O + oxaloacetate the enzyme also performs the citrate synthase reaction, EC 2.3.3.1 Aspergillus fumigatus citrate + CoA
-
 ?
2.3.3.5 additional information key enzyme of the methylcitrate cycle and required for fungal propionate degradation Aspergillus fumigatus ?
-
 ?
2.3.3.5 propanoyl-CoA + H2O + oxaloacetate reaction is followed by isomerization of (2S,3S)-2-methylcitrate, i.e. (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate, to (2R,3S)-2-methylisocitrate, i.e. (2R,3S)-2-hydroxybutane-1,2,3-tricarboxylate, via cis-2-methylaconitate Aspergillus fumigatus (2S,3S)-2-methylcitrate + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.3.5 ? x * 45000, native enzyme, SDS-PAGE, x * 514100, sequence calculation Aspergillus fumigatus

Synonyms

EC Number Synonyms Comment Organism
2.3.3.5 Methylcitrate synthase
-
 Aspergillus fumigatus
2.3.3.5 MscA
-
 Aspergillus fumigatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.3.5 50 60
-
 Aspergillus fumigatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.3.5 40
-
 3 h, stable up to Aspergillus fumigatus
2.3.3.5 60
-
 half-life 11 min Aspergillus fumigatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.3.5 8 9
-
 Aspergillus fumigatus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.3.3.5 5 9 3 h, stable Aspergillus fumigatus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.3.5 0.053
-
 propionyl-CoA versus CoA Aspergillus fumigatus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.3.3.5 Aspergillus fumigatus 2 peaks
-
 6.9
2.3.3.5 Aspergillus fumigatus 2 peaks
-
 9