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Literature summary extracted from

  • Yun, Y.S.; Nam, G.H.; Kim, Y.G.; Oh, B.H.; Choi, K.Y.
    Small exterior hydrophobic cluster contributes to conformational stability and steroid binding in ketosteroid isomerase from Pseudomonas putida biotype B (2005), FEBS J., 272, 1999-2011.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.3.3.1 mutant W92A, in complex with d-equilenin, decrease in conformational stability results from destabilization of surface hydrophobic layer Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
5.3.3.1 L125A/V127A mutation in small exterior hydrophobic cluster, decrease in conformational stability Pseudomonas putida
5.3.3.1 L125F/V127F mutation in small exterior hydrophobic cluster, slight increase in stability Pseudomonas putida
5.3.3.1 W92A mutation in small exterior hydrophobic cluster, decrease in conformational stability. Crystallization data Pseudomonas putida
5.3.3.1 W92A/L125A/V127A mutation in small exterior hydrophobic cluster, decrease in conformational stability Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.3.1 0.0499
-
 5-androstene-3,17-dione wild-type, 25°C, pH 7.0 Pseudomonas putida
5.3.3.1 0.0795
-
 5-androstene-3,17-dione mutant L125F/V127F, 25°C, pH 7.0 Pseudomonas putida
5.3.3.1 0.0995
-
 5-androstene-3,17-dione mutant W92A, 25°C, pH 7.0 Pseudomonas putida
5.3.3.1 0.1185
-
 5-androstene-3,17-dione mutant W92A/L125A/V127A, 25°C, pH 7.0 Pseudomonas putida
5.3.3.1 0.1375
-
 5-androstene-3,17-dione mutant L125A/V127A, 25°C, pH 7.0 Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
5.3.3.1 Pseudomonas putida
-
 biotype B
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.3.1 5-Androstene-3,17-dione
-
 Pseudomonas putida 4-Androstene-3,17-dione
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.3.1 12000
-
 5-androstene-3,17-dione mutant W92A/L125A/V127A, 25°C, pH 7.0 Pseudomonas putida
5.3.3.1 12700
-
 5-androstene-3,17-dione mutant W92A, 25°C, pH 7.0 Pseudomonas putida
5.3.3.1 12900
-
 5-androstene-3,17-dione mutant L125A/V127A, 25°C, pH 7.0 Pseudomonas putida
5.3.3.1 17600
-
 5-androstene-3,17-dione mutant L125F/V127F, 25°C, pH 7.0 Pseudomonas putida
5.3.3.1 21200
-
 5-androstene-3,17-dione wild-type, 25°C, pH 7.0 Pseudomonas putida