EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.4.2.8 | additional information | activation by substrate and product IMP, reversibly destabilizes the enzyme | Plasmodium falciparum |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.2.8 | expression of wild-type and mutant enzyme in Escherichia coli, complementation of an enzyme-deficient Escherichia coli strain by the wild-type and mutant L44F at growth temperatures 20°C and 37°C, and at 42°C only by the wild-type enzyme | Plasmodium falciparum |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.4.2.8 | L44F | site-directed mutagenesis, temperature-sensitive mutant, no complementation of an enzyme-deficient Escherichia coli strain at 42°C, only at 20°C and 37°C | Plasmodium falciparum |
EC Number | General Stability | Organism |
---|---|---|
2.4.2.8 | activation by substrate and product IMP destabilizes the enzyme, the unactivated ligand-free enzyme is more stable | Plasmodium falciparum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.2.8 | additional information | - |
additional information | - |
Plasmodium falciparum |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.4.2.8 | Mg2+ | required | Plasmodium falciparum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.2.8 | guanine + 5-phospho-alpha-D-ribose 1-diphosphate | Plasmodium falciparum | - |
GMP + diphosphate | - |
r | |
2.4.2.8 | hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | Plasmodium falciparum | - |
IMP + diphosphate | - |
r | |
2.4.2.8 | additional information | Plasmodium falciparum | the enzyme activity is regulated by stabilization and destabilization of the enzyme through release or binding of product and substrates, overview | ? | - |
? | |
2.4.2.8 | xanthine + 5-phospho-alpha-D-ribose 1-diphosphate | Plasmodium falciparum | low activity | xanthosine 5'-phosphate + diphosphate | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.2.8 | Plasmodium falciparum | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.2.8 | recombinant wild-type and mutant L44F from Escherichia coli, the enzyme shows very low activity | Plasmodium falciparum |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.4.2.8 | IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | ordered bi bi kinetic mechanism with 5-phospho-alpha-D-ribose 1-diphosphate binding first followed by the purine base | Plasmodium falciparum |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.4.2.8 | 1.45 | - |
purified recombinant enzyme, substrate hypoxanthine, in presence of 0.008 mM IMP, after 6 h | Plasmodium falciparum |
2.4.2.8 | 5.22 | - |
purified recombinant enzyme, substrate hypoxanthine, in presence of 0.038 mM IMP, after 48 h | Plasmodium falciparum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.2.8 | guanine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Plasmodium falciparum | GMP + diphosphate | - |
r | |
2.4.2.8 | hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Plasmodium falciparum | IMP + diphosphate | - |
r | |
2.4.2.8 | additional information | the enzyme activity is regulated by stabilization and destabilization of the enzyme through release or binding of product and substrates, overview | Plasmodium falciparum | ? | - |
? | |
2.4.2.8 | additional information | the purified recombinant enzyme shows very low activity | Plasmodium falciparum | ? | - |
? | |
2.4.2.8 | xanthine + 5-phospho-alpha-D-ribose 1-diphosphate | low activity | Plasmodium falciparum | xanthosine 5'-phosphate + diphosphate | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.2.8 | HGPRT | - |
Plasmodium falciparum |
2.4.2.8 | hypoxanthine guanine phosphoribosyltransferase | - |
Plasmodium falciparum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.2.8 | additional information | - |
- |
Plasmodium falciparum |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.2.8 | additional information | - |
- |
Plasmodium falciparum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.2.8 | 7 | - |
assay at | Plasmodium falciparum |