BRENDA - Enzyme Database

Glycolysis in Entamoeba histolytica. Biochemical characterization of recombinant glycolytic enzymes and flux control analysis

Saavedra, E.; Encalada, R.; Pineda, E.; Jasso-Chavez, R.; Moreno-Sanchez, R.; FEBS J. 272, 1767-1783 (2005)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.7.1.90
-
Entamoeba histolytica
2.7.9.1
-
Entamoeba histolytica
5.4.2.12
-
Entamoeba histolytica
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.90
0.05
-
diphosphate
37C, pH 7.0
Entamoeba histolytica
2.7.1.90
0.38
-
diphosphate
37C, pH 6.0
Entamoeba histolytica
2.7.1.90
0.455
-
D-fructose 6-phosphate
37C, pH 7.0
Entamoeba histolytica
2.7.1.90
0.695
-
D-fructose 6-phosphate
37C, pH 6.0
Entamoeba histolytica
2.7.9.1
0.002
-
AMP
pH 6.0, 37C
Entamoeba histolytica
2.7.9.1
0.005
-
AMP
pH 5.8-6.4 (optimal pH), 37C
Entamoeba histolytica
2.7.9.1
0.02
-
AMP
pH 7.0, 37C
Entamoeba histolytica
2.7.9.1
0.02
-
phosphoenolpyruvate
pH 5.8-6.4 (optimal pH), 37C
Entamoeba histolytica
2.7.9.1
0.024
-
phosphoenolpyruvate
pH 7.0, 37C
Entamoeba histolytica
2.7.9.1
0.03
-
phosphoenolpyruvate
pH 6.0, 37C
Entamoeba histolytica
2.7.9.1
0.047
-
diphosphate
pH 7.0, 37C
Entamoeba histolytica
2.7.9.1
0.068
-
pyruvate
pH 7.0, 37C
Entamoeba histolytica
2.7.9.1
0.091
-
diphosphate
pH 6.0, 37C
Entamoeba histolytica
2.7.9.1
0.1
-
diphosphate
pH 5.8-6.4 (optimal pH), 37C
Entamoeba histolytica
2.7.9.1
0.284
-
ATP
pH 6.0, 37C
Entamoeba histolytica
2.7.9.1
305
-
pyruvate
pH 6.0, 37C
Entamoeba histolytica
5.4.2.12
0.066
-
2-phospho-D-glycerate
pH 7.0, 37C
Entamoeba histolytica
5.4.2.12
0.106
-
2-phospho-D-glycerate
pH 6.0, 37C
Entamoeba histolytica
5.4.2.12
0.5
-
3-phospho-D-glycerate
pH 7.0, 37C
Entamoeba histolytica
5.4.2.12
0.83
-
3-phospho-D-glycerate
pH 6.0, 37C
Entamoeba histolytica
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.9.1
100000
-
4 * 100000, SDS-PAGE
Entamoeba histolytica
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.90
Entamoeba histolytica
-
-
-
2.7.9.1
Entamoeba histolytica
-
-
-
5.4.2.12
Entamoeba histolytica
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.90
recombinant protein
Entamoeba histolytica
2.7.9.1
-
Entamoeba histolytica
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2.7.1.90
112
-
37C, pH 6.0
Entamoeba histolytica
2.7.1.90
298
-
37C, pH 7.0
Entamoeba histolytica
Storage Stability
EC Number
Storage Stability
Organism
2.7.1.90
-20C, 50% v/v glycerol, less than 50% loss of activity within 2 months
Entamoeba histolytica
2.7.9.1
-20C, 50% v/v glycerol, optimal storage condition, decay factor is 3 months
Entamoeba histolytica
5.4.2.12
-20C, 50% v/v glycerol, less than 50% loss of activity in 2 months
Entamoeba histolytica
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.90
diphosphate + D-fructose 6-phosphate
-
661689
Entamoeba histolytica
phosphate + D-fructose 1,6-diphosphate
-
-
-
r
2.7.1.90
phosphate + D-fructose 1,6-diphosphate
-
661689
Entamoeba histolytica
diphosphate + D-fructose 6-phosphate
-
-
-
r
2.7.9.1
ATP + pyruvate + phosphate
-
661689
Entamoeba histolytica
AMP + phosphoenolpyruvate + diphosphate
-
-
-
?
5.4.2.12
2-phospho-D-glycerate
-
661689
Entamoeba histolytica
3-phospho-D-glycerate
-
-
-
r
5.4.2.12
3-phospho-D-glycerate
-
661689
Entamoeba histolytica
2-phospho-D-glycerate
-
-
-
r
Subunits
EC Number
Subunits
Commentary
Organism
2.7.9.1
tetramer
4 * 100000, SDS-PAGE
Entamoeba histolytica
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.9.1
10
-
pyruvate
pH 6.0, 37C
Entamoeba histolytica
2.7.9.1
15
-
pyruvate
pH 7.0, 37C
Entamoeba histolytica
2.7.9.1
53
-
phosphoenolpyruvate
pH 7.0, 37C
Entamoeba histolytica
2.7.9.1
80
-
phosphoenolpyruvate
pH 5.8-6.4 (optimal pH)
Entamoeba histolytica
2.7.9.1
87
-
phosphoenolpyruvate
pH 6.0, 37C
Entamoeba histolytica
5.4.2.12
6.4
-
2-phospho-D-glycerate
pH 6.0, 37C
Entamoeba histolytica
5.4.2.12
14
-
2-phospho-D-glycerate
pH 7.0, 37C
Entamoeba histolytica
5.4.2.12
45
-
3-phospho-D-glycerate
pH 7.0, 37C
Entamoeba histolytica
5.4.2.12
57
-
3-phospho-D-glycerate
pH 6.0, 37C
Entamoeba histolytica
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.9.1
5.8
6.4
reaction with AMP, phosphoenolpyruvate and diphosphate
Entamoeba histolytica
2.7.9.1
7
-
reaction with pyruvate, ATP and phosphate
Entamoeba histolytica
5.4.2.12
5.8
6.2
formation of 2-phospho-D-glycerate
Entamoeba histolytica
5.4.2.12
7
-
formation of 3-phospho-D-glycerate
Entamoeba histolytica
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.1.90
6
8
synthesis of diphosphate
Entamoeba histolytica
2.7.1.90
6.8
7.4
synthesis of D-fructose 1,6-diphosphate
Entamoeba histolytica
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
5.4.2.12
additional information
2,3-bisphosphoglycerate-independent phosphoglycerate mutase
Entamoeba histolytica
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.90
-
Entamoeba histolytica
2.7.9.1
-
Entamoeba histolytica
5.4.2.12
-
Entamoeba histolytica
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
5.4.2.12
additional information
2,3-bisphosphoglycerate-independent phosphoglycerate mutase
Entamoeba histolytica
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.90
0.05
-
diphosphate
37C, pH 7.0
Entamoeba histolytica
2.7.1.90
0.38
-
diphosphate
37C, pH 6.0
Entamoeba histolytica
2.7.1.90
0.455
-
D-fructose 6-phosphate
37C, pH 7.0
Entamoeba histolytica
2.7.1.90
0.695
-
D-fructose 6-phosphate
37C, pH 6.0
Entamoeba histolytica
2.7.9.1
0.002
-
AMP
pH 6.0, 37C
Entamoeba histolytica
2.7.9.1
0.005
-
AMP
pH 5.8-6.4 (optimal pH), 37C
Entamoeba histolytica
2.7.9.1
0.02
-
AMP
pH 7.0, 37C
Entamoeba histolytica
2.7.9.1
0.02
-
phosphoenolpyruvate
pH 5.8-6.4 (optimal pH), 37C
Entamoeba histolytica
2.7.9.1
0.024
-
phosphoenolpyruvate
pH 7.0, 37C
Entamoeba histolytica
2.7.9.1
0.03
-
phosphoenolpyruvate
pH 6.0, 37C
Entamoeba histolytica
2.7.9.1
0.047
-
diphosphate
pH 7.0, 37C
Entamoeba histolytica
2.7.9.1
0.068
-
pyruvate
pH 7.0, 37C
Entamoeba histolytica
2.7.9.1
0.091
-
diphosphate
pH 6.0, 37C
Entamoeba histolytica
2.7.9.1
0.1
-
diphosphate
pH 5.8-6.4 (optimal pH), 37C
Entamoeba histolytica
2.7.9.1
0.284
-
ATP
pH 6.0, 37C
Entamoeba histolytica
2.7.9.1
305
-
pyruvate
pH 6.0, 37C
Entamoeba histolytica
5.4.2.12
0.066
-
2-phospho-D-glycerate
pH 7.0, 37C
Entamoeba histolytica
5.4.2.12
0.106
-
2-phospho-D-glycerate
pH 6.0, 37C
Entamoeba histolytica
5.4.2.12
0.5
-
3-phospho-D-glycerate
pH 7.0, 37C
Entamoeba histolytica
5.4.2.12
0.83
-
3-phospho-D-glycerate
pH 6.0, 37C
Entamoeba histolytica
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.9.1
100000
-
4 * 100000, SDS-PAGE
Entamoeba histolytica
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.90
recombinant protein
Entamoeba histolytica
2.7.9.1
-
Entamoeba histolytica
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2.7.1.90
112
-
37C, pH 6.0
Entamoeba histolytica
2.7.1.90
298
-
37C, pH 7.0
Entamoeba histolytica
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.7.1.90
-20C, 50% v/v glycerol, less than 50% loss of activity within 2 months
Entamoeba histolytica
2.7.9.1
-20C, 50% v/v glycerol, optimal storage condition, decay factor is 3 months
Entamoeba histolytica
5.4.2.12
-20C, 50% v/v glycerol, less than 50% loss of activity in 2 months
Entamoeba histolytica
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.90
diphosphate + D-fructose 6-phosphate
-
661689
Entamoeba histolytica
phosphate + D-fructose 1,6-diphosphate
-
-
-
r
2.7.1.90
phosphate + D-fructose 1,6-diphosphate
-
661689
Entamoeba histolytica
diphosphate + D-fructose 6-phosphate
-
-
-
r
2.7.9.1
ATP + pyruvate + phosphate
-
661689
Entamoeba histolytica
AMP + phosphoenolpyruvate + diphosphate
-
-
-
?
5.4.2.12
2-phospho-D-glycerate
-
661689
Entamoeba histolytica
3-phospho-D-glycerate
-
-
-
r
5.4.2.12
3-phospho-D-glycerate
-
661689
Entamoeba histolytica
2-phospho-D-glycerate
-
-
-
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.9.1
tetramer
4 * 100000, SDS-PAGE
Entamoeba histolytica
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.9.1
10
-
pyruvate
pH 6.0, 37C
Entamoeba histolytica
2.7.9.1
15
-
pyruvate
pH 7.0, 37C
Entamoeba histolytica
2.7.9.1
53
-
phosphoenolpyruvate
pH 7.0, 37C
Entamoeba histolytica
2.7.9.1
80
-
phosphoenolpyruvate
pH 5.8-6.4 (optimal pH)
Entamoeba histolytica
2.7.9.1
87
-
phosphoenolpyruvate
pH 6.0, 37C
Entamoeba histolytica
5.4.2.12
6.4
-
2-phospho-D-glycerate
pH 6.0, 37C
Entamoeba histolytica
5.4.2.12
14
-
2-phospho-D-glycerate
pH 7.0, 37C
Entamoeba histolytica
5.4.2.12
45
-
3-phospho-D-glycerate
pH 7.0, 37C
Entamoeba histolytica
5.4.2.12
57
-
3-phospho-D-glycerate
pH 6.0, 37C
Entamoeba histolytica
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.9.1
5.8
6.4
reaction with AMP, phosphoenolpyruvate and diphosphate
Entamoeba histolytica
2.7.9.1
7
-
reaction with pyruvate, ATP and phosphate
Entamoeba histolytica
5.4.2.12
5.8
6.2
formation of 2-phospho-D-glycerate
Entamoeba histolytica
5.4.2.12
7
-
formation of 3-phospho-D-glycerate
Entamoeba histolytica
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.1.90
6
8
synthesis of diphosphate
Entamoeba histolytica
2.7.1.90
6.8
7.4
synthesis of D-fructose 1,6-diphosphate
Entamoeba histolytica