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Literature summary extracted from

  • Saavedra, E.; Encalada, R.; Pineda, E.; Jasso-Chavez, R.; Moreno-Sanchez, R.
    Glycolysis in Entamoeba histolytica. Biochemical characterization of recombinant glycolytic enzymes and flux control analysis (2005), FEBS J., 272, 1767-1783.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.90
-
 Entamoeba histolytica
2.7.9.1
-
 Entamoeba histolytica
5.4.2.12
-
 Entamoeba histolytica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.90 0.05
-
 diphosphate 37°C, pH 7.0 Entamoeba histolytica
2.7.1.90 0.38
-
 diphosphate 37°C, pH 6.0 Entamoeba histolytica
2.7.1.90 0.455
-
 D-fructose 6-phosphate 37°C, pH 7.0 Entamoeba histolytica
2.7.1.90 0.695
-
 D-fructose 6-phosphate 37°C, pH 6.0 Entamoeba histolytica
2.7.9.1 0.002
-
 AMP pH 6.0, 37°C Entamoeba histolytica
2.7.9.1 0.005
-
 AMP pH 5.8-6.4 (optimal pH), 37°C Entamoeba histolytica
2.7.9.1 0.02
-
 AMP pH 7.0, 37°C Entamoeba histolytica
2.7.9.1 0.02
-
 phosphoenolpyruvate pH 5.8-6.4 (optimal pH), 37°C Entamoeba histolytica
2.7.9.1 0.024
-
 phosphoenolpyruvate pH 7.0, 37°C Entamoeba histolytica
2.7.9.1 0.03
-
 phosphoenolpyruvate pH 6.0, 37°C Entamoeba histolytica
2.7.9.1 0.047
-
 diphosphate pH 7.0, 37°C Entamoeba histolytica
2.7.9.1 0.068
-
 pyruvate pH 7.0, 37°C Entamoeba histolytica
2.7.9.1 0.091
-
 diphosphate pH 6.0, 37°C Entamoeba histolytica
2.7.9.1 0.1
-
 diphosphate pH 5.8-6.4 (optimal pH), 37°C Entamoeba histolytica
2.7.9.1 0.284
-
 ATP pH 6.0, 37°C Entamoeba histolytica
2.7.9.1 305
-
 pyruvate pH 6.0, 37°C Entamoeba histolytica
5.4.2.12 0.066
-
 2-phospho-D-glycerate pH 7.0, 37°C Entamoeba histolytica
5.4.2.12 0.106
-
 2-phospho-D-glycerate pH 6.0, 37°C Entamoeba histolytica
5.4.2.12 0.5
-
 3-phospho-D-glycerate pH 7.0, 37°C Entamoeba histolytica
5.4.2.12 0.83
-
 3-phospho-D-glycerate pH 6.0, 37°C Entamoeba histolytica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.9.1 100000
-
 4 * 100000, SDS-PAGE Entamoeba histolytica

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.90 Entamoeba histolytica
-
-
-
2.7.9.1 Entamoeba histolytica
-
-
-
5.4.2.12 Entamoeba histolytica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.90 recombinant protein Entamoeba histolytica
2.7.9.1
-
 Entamoeba histolytica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.90 112
-
 37°C, pH 6.0 Entamoeba histolytica
2.7.1.90 298
-
 37°C, pH 7.0 Entamoeba histolytica

Storage Stability

EC Number Storage Stability Organism
2.7.1.90 -20°C, 50% v/v glycerol, less than 50% loss of activity within 2 months Entamoeba histolytica
2.7.9.1 -20°C, 50% v/v glycerol, optimal storage condition, decay factor is 3 months Entamoeba histolytica
5.4.2.12 -20°C, 50% v/v glycerol, less than 50% loss of activity in 2 months Entamoeba histolytica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.90 diphosphate + D-fructose 6-phosphate
-
 Entamoeba histolytica phosphate + D-fructose 1,6-diphosphate
-
 r
2.7.1.90 phosphate + D-fructose 1,6-diphosphate
-
 Entamoeba histolytica diphosphate + D-fructose 6-phosphate
-
 r
2.7.9.1 ATP + pyruvate + phosphate
-
 Entamoeba histolytica AMP + phosphoenolpyruvate + diphosphate
-
 ?
5.4.2.12 2-phospho-D-glycerate
-
 Entamoeba histolytica 3-phospho-D-glycerate
-
 r
5.4.2.12 3-phospho-D-glycerate
-
 Entamoeba histolytica 2-phospho-D-glycerate
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.9.1 tetramer 4 * 100000, SDS-PAGE Entamoeba histolytica

Synonyms

EC Number Synonyms Comment Organism
2.7.9.1 PPDK
-
 Entamoeba histolytica

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.9.1 10
-
 pyruvate pH 6.0, 37°C Entamoeba histolytica
2.7.9.1 15
-
 pyruvate pH 7.0, 37°C Entamoeba histolytica
2.7.9.1 53
-
 phosphoenolpyruvate pH 7.0, 37°C Entamoeba histolytica
2.7.9.1 80
-
 phosphoenolpyruvate pH 5.8-6.4 (optimal pH) Entamoeba histolytica
2.7.9.1 87
-
 phosphoenolpyruvate pH 6.0, 37°C Entamoeba histolytica
5.4.2.12 6.4
-
 2-phospho-D-glycerate pH 6.0, 37°C Entamoeba histolytica
5.4.2.12 14
-
 2-phospho-D-glycerate pH 7.0, 37°C Entamoeba histolytica
5.4.2.12 45
-
 3-phospho-D-glycerate pH 7.0, 37°C Entamoeba histolytica
5.4.2.12 57
-
 3-phospho-D-glycerate pH 6.0, 37°C Entamoeba histolytica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.9.1 5.8 6.4 reaction with AMP, phosphoenolpyruvate and diphosphate Entamoeba histolytica
2.7.9.1 7
-
 reaction with pyruvate, ATP and phosphate Entamoeba histolytica
5.4.2.12 5.8 6.2 formation of 2-phospho-D-glycerate Entamoeba histolytica
5.4.2.12 7
-
 formation of 3-phospho-D-glycerate Entamoeba histolytica

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.90 6 8 synthesis of diphosphate Entamoeba histolytica
2.7.1.90 6.8 7.4 synthesis of D-fructose 1,6-diphosphate Entamoeba histolytica

Cofactor

EC Number Cofactor Comment Organism Structure
5.4.2.12 additional information 2,3-bisphosphoglycerate-independent phosphoglycerate mutase Entamoeba histolytica