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Literature summary extracted from
- AMP-forming acetyl-CoA synthetase from the extremely halophilic archaeon Haloarcula marismortui: purification, identification and expression of the encoding gene, and phylogenetic affiliation (2005), Extremophiles, 9, 355-365.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 6.2.1.1 | overexpression in Escherichia coli | Haloarcula marismortui |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 6.2.1.1 | KCl | optimal activity at 1-1.5 M | Haloarcula marismortui |  |
| 6.2.1.1 | Mg2+ | optimal concentration: 5 mM in presence of 1.25 mM NaCl | Haloarcula marismortui | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 6.2.1.1 | 72000 | - |
gel filtration | Haloarcula marismortui |
| 6.2.1.1 | 89000 | - |
1 * 89000, SDS-PAGE | Haloarcula marismortui |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 6.2.1.1 | Haloarcula marismortui | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 6.2.1.1 | - |
Haloarcula marismortui |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 6.2.1.1 | 13.4 | - |
formation of acetyl-CoA | Haloarcula marismortui |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 6.2.1.1 | ATP + acetate + CoA | - |
Haloarcula marismortui | AMP + diphosphate + acetyl-CoA | - |
r | |
| 6.2.1.1 | ATP + propanoate + CoA | 30% of the activity with acetate | Haloarcula marismortui | AMP + diphosphate + propanoyl-CoA | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 6.2.1.1 | monomer | 1 * 89000, SDS-PAGE | Haloarcula marismortui |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 6.2.1.1 | AMP-forming acetyl-CoA synthetase | - |
Haloarcula marismortui |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 6.2.1.1 | 41 | - |
- |
Haloarcula marismortui |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 6.2.1.1 | 7.5 | - |
- |
Haloarcula marismortui |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 6.2.1.1 | 6.5 | 8.5 | 30-40% of maximal activity at pH 6.5 and 8.5 | Haloarcula marismortui |
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