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Literature summary extracted from

  • Hansen, T.; Urbanke, C.; Schonheit, P.
    Bifunctional phosphoglucose/phosphomannose isomerase from the hyperthermophilic archaeon Pyrobaculum aerophilum (2004), Extremophiles, 8, 507-512.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.8
-
 Pyrobaculum aerophilum
5.3.1.9
-
 Pyrobaculum aerophilum

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.1.8 6-phosphogluconate
-
 Pyrobaculum aerophilum
5.3.1.8 erythrose 4-phosphate
-
 Pyrobaculum aerophilum
5.3.1.9 6-phosphogluconate
-
 Pyrobaculum aerophilum
5.3.1.9 erythrose-4-phosphate
-
 Pyrobaculum aerophilum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.8 0.06
-
 D-fructose 6-phosphate pH 7.4, 80°C Pyrobaculum aerophilum
5.3.1.8 0.06
-
 D-mannose 6-phosphate pH 7.4, 80°C Pyrobaculum aerophilum
5.3.1.8 0.3
-
 D-fructose 6-phosphate pH 7.4, 50°C Pyrobaculum aerophilum
5.3.1.9 0.06
-
 D-fructose 6-phosphate pH 7.4, 80°C Pyrobaculum aerophilum
5.3.1.9 0.3
-
 D-fructose 6-phosphate pH 7.4, 50°C Pyrobaculum aerophilum
5.3.1.9 1.9
-
 D-glucose 6-phosphate pH 7.4, 80°C Pyrobaculum aerophilum
5.3.1.9 2.7
-
 D-glucose 6-phosphate pH 7.4, 50°C Pyrobaculum aerophilum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.8 33548
-
 2 * 36000, SDS-PAGE, 2 * 33548, calculated Pyrobaculum aerophilum
5.3.1.8 36000
-
 2 * 36000, SDS-PAGE, 2 * 33548, calculated Pyrobaculum aerophilum
5.3.1.8 65000
-
 sedimentation equilibrium, analytical ultracentrifugation Pyrobaculum aerophilum
5.3.1.9 33548
-
 2 * 36000, SDS-PAGE, 2 * 33548, calculated Pyrobaculum aerophilum
5.3.1.9 36000
-
 2 * 36000, SDS-PAGE, 2 * 33548, calculated Pyrobaculum aerophilum
5.3.1.9 65000
-
 sedimentation equilibrium, analytical ultracentrifugation Pyrobaculum aerophilum

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.8 Pyrobaculum aerophilum
-
 bifunctional phosphoglucose/phosphomannose isomerase EC 5.3.1.8 and EC 5.3.1.9, overexpression in Escherichia coli
-
5.3.1.9 Pyrobaculum aerophilum
-
 bifunctional phosphoglucose/phosphomannose isomerase EC 5.3.1.8 and EC5.3.1.9, overexpression in Escherichia coli
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.8 D-fructose 6-phosphate
-
 Pyrobaculum aerophilum D-mannose 6-phosphate
-
 r
5.3.1.8 D-Mannose 6-phosphate
-
 Pyrobaculum aerophilum D-Fructose 6-phosphate
-
 r
5.3.1.9 D-fructose 6-phosphate
-
 Pyrobaculum aerophilum D-glucose 6-phosphate
-
 r
5.3.1.9 D-glucose 6-phosphate
-
 Pyrobaculum aerophilum D-fructose 6-phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
5.3.1.8 dimer 2 * 36000, SDS-PAGE, 2 * 33548, calculated Pyrobaculum aerophilum
5.3.1.9 dimer 2 * 36000, SDS-PAGE, 2 * 33548, calculated Pyrobaculum aerophilum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.1.8 100
-
-
 Pyrobaculum aerophilum
5.3.1.9 100
-
-
 Pyrobaculum aerophilum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.3.1.8 100
-
 melting temperature for thermal unfolding, 60 min, 50% residual activity Pyrobaculum aerophilum
5.3.1.9 100
-
 melting temperature for thermal unfolding, 60 min, 50% residual activity Pyrobaculum aerophilum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.8 7.4
-
-
 Pyrobaculum aerophilum
5.3.1.9 7.4
-
-
 Pyrobaculum aerophilum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.3.1.8 6.3 8.8 more than 50% of maximum activity in this range Pyrobaculum aerophilum
5.3.1.9 6.3 8.8 more than 50% of maximum activity in this range Pyrobaculum aerophilum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
5.3.1.8 0.0014
-
 erythrose-4-phosphate pH 7.4, 50°C Pyrobaculum aerophilum
5.3.1.8 0.11
-
 6-phosphogluconate pH 7.4, 50°C Pyrobaculum aerophilum
5.3.1.9 0.0014
-
 erythrose-4-phosphate pH 7.4, 50°C Pyrobaculum aerophilum
5.3.1.9 0.11
-
 6-phosphogluconate pH 7.4, 50°C Pyrobaculum aerophilum