Literature summary extracted from

Kudryashov, D.S.; Stepanova, O.V.; Vilitkevich, E.L.; Nikonenko, T.A.; Nadezhdina, E.S.; Shanina, N.A.; Lukas, T.J.; Van Eldik, L.J.; Watterson, D.M.; Shirinsky, V.P.
Myosin light chain kinase (210 kDa) is a potential cytoskeleton integrator through its unique N-terminal domain (2004), Exp. Cell Res., 298, 407-417.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
2.7.11.18	expression of His6-tagged MLCK-210 nad His-tagged N452-MLCK in Escherichia coli, expression of GFP-tagged MLCK-210 and GFP-tagged N452-MLCK in CV-1 African green monkey kidney cells	Gallus gallus

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
2.7.11.18	210000	-	-	Gallus gallus

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2.7.11.18	additional information	Gallus gallus	the enzyme is a potential cytoskeleton integrator through its unique N-terminal domain, the enzyme binds tubulin and actin, and is involved in microtubule/microfilament association	?	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.7.11.18	Gallus gallus	-	-	-

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
2.7.11.18	aorta	-	Gallus gallus	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.7.11.18	additional information	the enzyme is a potential cytoskeleton integrator through its unique N-terminal domain, the enzyme binds tubulin and actin, and is involved in microtubule/microfilament association	Gallus gallus	?	-	?

Subunits

EC Number	Subunits	Comment	Organism
2.7.11.18	More	MLCK-210 contains an additional actin-binding domain at the N-terminus	Gallus gallus

Synonyms

EC Number	Synonyms	Comment	Organism
2.7.11.18	MLCK-210	-	Gallus gallus
2.7.11.18	myosin light chain kinase	-	Gallus gallus

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
2.7.11.18	ATP	-	Gallus gallus
2.7.11.18	Calmodulin	-	Gallus gallus

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Release 2023.1 (January 2023)