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Literature summary extracted from

  • Yamashita, T.; Ashiuchi, M.; Ohnishi, K.; Kato, S.; Nagata, S.; Misono, H.
    Molecular identification of monomeric aspartate racemase from Bifidobacterium bifidum (2004), Eur. J. Biochem., 271, 4798-4803.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.1.13 overexpression in Escherichia coli Bifidobacterium bifidum

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.1.1.13 5,5'-dithiobis(2-nitrobenzoate) 1 mM, complete inhibition, pH 7.0, 30°C, 30 min Bifidobacterium bifidum
5.1.1.13 alpha-methyl-DL-aspartic acid 10 mM, 12% inhibition Bifidobacterium bifidum
5.1.1.13 HgCl2 0.1 M, complete inhibition, pH 7.0, 30°C, 30 min Bifidobacterium bifidum
5.1.1.13 NEM 1 mM, complete inhibition, pH 7.0, 30°C, 30 min Bifidobacterium bifidum
5.1.1.13 PCMB 0.1 M, complete inhibition, pH 7.0, 30°C, 30 min Bifidobacterium bifidum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.1.13 0.94
-
 D-Asp
-
 Bifidobacterium bifidum
5.1.1.13 13.4
-
 L-Asp
-
 Bifidobacterium bifidum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.1.13 27000
-
 gel filtration Bifidobacterium bifidum
5.1.1.13 30000
-
 1 * 30000, SDS-PAGE Bifidobacterium bifidum

Organism

EC Number Organism UniProt Comment Textmining
5.1.1.13 Bifidobacterium bifidum
-
 NBRC 14252
-
5.1.1.13 Bifidobacterium bifidum NBRC 14252
-
 NBRC 14252
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.1.13
-
 Bifidobacterium bifidum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.1.1.13 4.75
-
-
 Bifidobacterium bifidum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.1.13 L-Asp enzyme exclusively acts on L-Asp and D-Asp Bifidobacterium bifidum D-Asp
-
 r
5.1.1.13 L-Asp enzyme exclusively acts on L-Asp and D-Asp Bifidobacterium bifidum NBRC 14252 D-Asp
-
 r

Subunits

EC Number Subunits Comment Organism
5.1.1.13 monomer 1 * 30000, SDS-PAGE Bifidobacterium bifidum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.1.1.13 45
-
-
 Bifidobacterium bifidum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.1.1.13 30
-
 30 min, stable up to 30°C in 0.1 M Tes buffer, pH 6.5 containing 4 mM dithiothreitol and 1 mM EDTA Bifidobacterium bifidum
5.1.1.13 60
-
 30 min, stable up to 60°C in presence of 40 mM D-Asp Bifidobacterium bifidum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.1.13 7 7.5
-
 Bifidobacterium bifidum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
5.1.1.13 6 7 30°C, 30 min, stable Bifidobacterium bifidum

Cofactor

EC Number Cofactor Comment Organism Structure
5.1.1.13 additional information enzyme does not require a cofactor Bifidobacterium bifidum