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Literature summary extracted from
- Characterization of the cofactor-independent phosphoglycerate mutase from Leishmania mexicana mexicana. Histidines that coordinate the two metal ions in the active site show different susceptibilities to irreversible chemical modification (2004), Eur. J. Biochem., 271, 1798-1810.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.4.2.12 | expression in Escherichia coli with C-terminal His-tag | Leishmania mexicana |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 5.4.2.12 | rapid decrease in activity when no stabilizer is added. In presence of NaCl, CoCl2, and imidazole, stable for 1 month at 4°C | Leishmania mexicana |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.2.12 | diethyl dicarbonate | irreversible inhibition, saturating concentrations of substrate protect | Leishmania mexicana |  |
| 5.4.2.12 | EDTA | 1 mM, 10% residual activity at pH 8.0, reversible by incubation with CoCl2, but not MnCl2, FeSO4, CuSo4, NiCl2, ZnCl2 | Leishmania mexicana | |
| 5.4.2.12 | additional information | not inhibitory: vanadate, cloride, sulfate | Leishmania mexicana | |
| 5.4.2.12 | phosphate | 100 mM, 70% residual activity | Leishmania mexicana | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.2.12 | 0.11 | - |
2-phospho-D-glycerate | pH 7.6, 25°C | Leishmania mexicana | |
| 5.4.2.12 | 0.27 | - |
3-phospho-D-glycerate | pH 7.6, 25°C | Leishmania mexicana | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 5.4.2.12 | cytosol | - |
Leishmania mexicana | 5829 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.2.12 | CoCl2 | inhibition by EDTA is reversible by incubation with CoCl2, but not MnCl2, FeSO4, CuSo4, NiCl2, ZnCl2 | Leishmania mexicana | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.4.2.12 | 60000 | - |
x * 60724, calculated, x * 60000, recombinant protein with His-tag, SDS-PAGE | Leishmania mexicana |
| 5.4.2.12 | 60724 | - |
x * 60724, calculated, x * 60000, recombinant protein with His-tag, SDS-PAGE | Leishmania mexicana |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.2.12 | Leishmania mexicana | Q86N96 | sp. mexicana | - |
Renatured (Commentary)
| EC Number | Renatured (Comment) | Organism |
|---|---|---|
| 5.4.2.12 | inactivation by EDTA is reversible by incubation with CoCl2, but not MnCl2, FeSO4, CuSo4,NiCl2, ZnCl2 | Leishmania mexicana |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.4.2.12 | 419 | - |
formation of 2-phospho-D-glycerate, pH 7.6, 25°C | Leishmania mexicana |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.2.12 | 2-phospho-D-glycerate | - |
Leishmania mexicana | 3-phospho-D-glycerate | - |
r | |
| 5.4.2.12 | 3-phospho-D-glycerate | - |
Leishmania mexicana | 2-phospho-D-glycerate | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.4.2.12 | ? | x * 60724, calculated, x * 60000, recombinant protein with His-tag, SDS-PAGE | Leishmania mexicana |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.2.12 | 199 | - |
2-phospho-D-glycerate | pH 7.6, 25°C | Leishmania mexicana | |
| 5.4.2.12 | 434 | - |
3-phospho-D-glycerate | pH 7.6, 25°C | Leishmania mexicana | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.4.2.12 | 7.5 | 8.2 | for both directions of reaction | Leishmania mexicana |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.4.2.12 | 6.8 | 8.8 | more than 75% of maximum activity within this range, formation of 2-phospho-D-glycerate | Leishmania mexicana |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.2.12 | additional information | 2,3-bisphosphoglycerate independent phosphoglycerate mutase | Leishmania mexicana |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 5.4.2.12 | Leishmania mexicana | calculated | - |
5.26 |
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