BRENDA - Enzyme Database

Characterization of the cofactor-independent phosphoglycerate mutase from Leishmania mexicana mexicana. Histidines that coordinate the two metal ions in the active site show different susceptibilities to irreversible chemical modification

Guerra, D.G.; Vertommen, D.; Fothergill-Gilmore, L.A.; Opperdoes, F.R.; Michels, P.A.; Eur. J. Biochem. 271, 1798-1810 (2004)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
5.4.2.12
expression in Escherichia coli with C-terminal His-tag
Leishmania mexicana
General Stability
EC Number
General Stability
Organism
5.4.2.12
rapid decrease in activity when no stabilizer is added. In presence of NaCl, CoCl2, and imidazole, stable for 1 month at 4°C
Leishmania mexicana
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
5.4.2.12
diethyl dicarbonate
irreversible inhibition, saturating concentrations of substrate protect
Leishmania mexicana
5.4.2.12
EDTA
1 mM, 10% residual activty at pH 8.0, reversible by incubation with CoCl2, but not MnCl2, FeSO4, CuSo4, NiCl2, ZnCl2
Leishmania mexicana
5.4.2.12
additional information
not inhibitory: vanadate, cloride, sulfate
Leishmania mexicana
5.4.2.12
phosphate
100 mM, 70% residual activity
Leishmania mexicana
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.4.2.12
0.11
-
2-phospho-D-glycerate
pH 7.6, 25°C
Leishmania mexicana
5.4.2.12
0.27
-
3-phospho-D-glycerate
pH 7.6, 25°C
Leishmania mexicana
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
5.4.2.12
cytosol
-
Leishmania mexicana
5829
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
5.4.2.12
CoCl2
inhibition by EDTA is reversible by incubation with CoCl2, but not MnCl2, FeSO4, CuSo4, NiCl2, ZnCl2
Leishmania mexicana
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.4.2.12
60000
-
x * 60724, calculated, x * 60000, recombinant protein with His-tag, SDS-PAGE
Leishmania mexicana
5.4.2.12
60724
-
x * 60724, calculated, x * 60000, recombinant protein with His-tag, SDS-PAGE
Leishmania mexicana
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.4.2.12
Leishmania mexicana
Q86N96
sp. mexicana
-
Renatured (Commentary)
EC Number
Commentary
Organism
5.4.2.12
inactivation by EDTA is reversible by incubation with CoCl2, but not MnCl2, FeSO4, CuSo4,NiCl2, ZnCl2
Leishmania mexicana
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
5.4.2.12
419
-
formation of 2-phospho-D-glycerate, pH 7.6, 25°C
Leishmania mexicana
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.2.12
2-phospho-D-glycerate
-
661634
Leishmania mexicana
3-phospho-D-glycerate
-
-
-
r
5.4.2.12
3-phospho-D-glycerate
-
661634
Leishmania mexicana
2-phospho-D-glycerate
-
-
-
r
Subunits
EC Number
Subunits
Commentary
Organism
5.4.2.12
?
x * 60724, calculated, x * 60000, recombinant protein with His-tag, SDS-PAGE
Leishmania mexicana
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
5.4.2.12
199
-
2-phospho-D-glycerate
pH 7.6, 25°C
Leishmania mexicana
5.4.2.12
434
-
3-phospho-D-glycerate
pH 7.6, 25°C
Leishmania mexicana
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.4.2.12
7.5
8.2
for both directions of reaction
Leishmania mexicana
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
5.4.2.12
6.8
8.8
more than 75% of maximum activity within this range, formation of 2-phospho-D-glycerate
Leishmania mexicana
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
5.4.2.12
additional information
2,3-bisphosphoglycerate independent phosphoglycerate mutase
Leishmania mexicana
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
5.4.2.12
Leishmania mexicana
calculated
-
5.26
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
5.4.2.12
expression in Escherichia coli with C-terminal His-tag
Leishmania mexicana
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
5.4.2.12
additional information
2,3-bisphosphoglycerate independent phosphoglycerate mutase
Leishmania mexicana
General Stability (protein specific)
EC Number
General Stability
Organism
5.4.2.12
rapid decrease in activity when no stabilizer is added. In presence of NaCl, CoCl2, and imidazole, stable for 1 month at 4°C
Leishmania mexicana
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
5.4.2.12
diethyl dicarbonate
irreversible inhibition, saturating concentrations of substrate protect
Leishmania mexicana
5.4.2.12
EDTA
1 mM, 10% residual activty at pH 8.0, reversible by incubation with CoCl2, but not MnCl2, FeSO4, CuSo4, NiCl2, ZnCl2
Leishmania mexicana
5.4.2.12
additional information
not inhibitory: vanadate, cloride, sulfate
Leishmania mexicana
5.4.2.12
phosphate
100 mM, 70% residual activity
Leishmania mexicana
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.4.2.12
0.11
-
2-phospho-D-glycerate
pH 7.6, 25°C
Leishmania mexicana
5.4.2.12
0.27
-
3-phospho-D-glycerate
pH 7.6, 25°C
Leishmania mexicana
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
5.4.2.12
cytosol
-
Leishmania mexicana
5829
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
5.4.2.12
CoCl2
inhibition by EDTA is reversible by incubation with CoCl2, but not MnCl2, FeSO4, CuSo4, NiCl2, ZnCl2
Leishmania mexicana
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.4.2.12
60000
-
x * 60724, calculated, x * 60000, recombinant protein with His-tag, SDS-PAGE
Leishmania mexicana
5.4.2.12
60724
-
x * 60724, calculated, x * 60000, recombinant protein with His-tag, SDS-PAGE
Leishmania mexicana
Renatured (Commentary) (protein specific)
EC Number
Commentary
Organism
5.4.2.12
inactivation by EDTA is reversible by incubation with CoCl2, but not MnCl2, FeSO4, CuSo4,NiCl2, ZnCl2
Leishmania mexicana
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
5.4.2.12
419
-
formation of 2-phospho-D-glycerate, pH 7.6, 25°C
Leishmania mexicana
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.2.12
2-phospho-D-glycerate
-
661634
Leishmania mexicana
3-phospho-D-glycerate
-
-
-
r
5.4.2.12
3-phospho-D-glycerate
-
661634
Leishmania mexicana
2-phospho-D-glycerate
-
-
-
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
5.4.2.12
?
x * 60724, calculated, x * 60000, recombinant protein with His-tag, SDS-PAGE
Leishmania mexicana
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
5.4.2.12
199
-
2-phospho-D-glycerate
pH 7.6, 25°C
Leishmania mexicana
5.4.2.12
434
-
3-phospho-D-glycerate
pH 7.6, 25°C
Leishmania mexicana
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.4.2.12
7.5
8.2
for both directions of reaction
Leishmania mexicana
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
5.4.2.12
6.8
8.8
more than 75% of maximum activity within this range, formation of 2-phospho-D-glycerate
Leishmania mexicana
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
5.4.2.12
Leishmania mexicana
calculated
-
5.26