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Literature summary extracted from

  • Chopra, P.; Singh, A.; Koul, A.; Ramachandran, S.; Drlica, K.; Tyagi, A.K.; Singh, Y.
    Cytotoxic activity of nucleoside diphosphate kinase secreted from Mycobacterium tuberculosis (2003), Eur. J. Biochem., 270, 625-634.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.6 native and mutant enzymes expressed as His-tag fusion protein in Escherichia coli SG13009 Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
2.7.4.6 H117Q significant loss of activity with ATP and GTP Mycobacterium tuberculosis
2.7.4.6 H49Q no effect on activity Mycobacterium tuberculosis
2.7.4.6 H53Q no effect on activity Mycobacterium tuberculosis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.4.6 extracellular enzyme is secreted to the culture medium Mycobacterium tuberculosis
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.6 14400
-
 x * 14400, SDS-PAGE Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.6 ATP + NDP Mycobacterium tuberculosis contributes to the maintenance of the cellular pools of all nucleosides triphosphates, extracellular enzyme contributes to ATP-induced cell death of macrophages ADP + NTP
-
 ?
2.7.4.6 ATP + NDP Mycobacterium tuberculosis H37Rv contributes to the maintenance of the cellular pools of all nucleosides triphosphates, extracellular enzyme contributes to ATP-induced cell death of macrophages ADP + NTP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.6 Mycobacterium tuberculosis
-
-
-
2.7.4.6 Mycobacterium tuberculosis H37Rv
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.6 recombinant enzymes using His-tag Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.6 ATP + NDP
-
 Mycobacterium tuberculosis ADP + NTP
-
 ?
2.7.4.6 ATP + NDP contributes to the maintenance of the cellular pools of all nucleosides triphosphates, extracellular enzyme contributes to ATP-induced cell death of macrophages Mycobacterium tuberculosis ADP + NTP
-
 ?
2.7.4.6 ATP + NDP
-
 Mycobacterium tuberculosis H37Rv ADP + NTP
-
 ?
2.7.4.6 ATP + NDP contributes to the maintenance of the cellular pools of all nucleosides triphosphates, extracellular enzyme contributes to ATP-induced cell death of macrophages Mycobacterium tuberculosis H37Rv ADP + NTP
-
 ?
2.7.4.6 GTP + NDP
-
 Mycobacterium tuberculosis GDP + NTP
-
 ?
2.7.4.6 GTP + NDP
-
 Mycobacterium tuberculosis H37Rv GDP + NTP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.4.6 ? x * 14400, SDS-PAGE Mycobacterium tuberculosis