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Literature summary extracted from

  • Berg, H.; Ziegler, K.; Piotukh, K.; Baier, K.; Lockau, W.; Volkmer-Engert, R.
    Biosynthesis of the cyanobacterial reserve polymer multi-L-arginyl-poly-L-aspartic acid (cyanophycin) (2000), Eur. J. Biochem., 267, 5561-5570.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.29 expression in Escherichia coli BL21(DE3) Trichormus variabilis ATCC 29413
6.3.2.30 expression in Escherichia coli BL21(DE3) Trichormus variabilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.29 0.015
-
 L-Arg 28°C, pH 8.2 Trichormus variabilis ATCC 29413

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.29 [L-Asp(4-L-Arg)]n + L-Asp + ATP Trichormus variabilis ATCC 29413 a small amount of cyanophycin is required as a primer [L-Asp(4-L-Arg)]n-Asp + ADP + phosphate no formation of AMP, [L-Asp(4-L-Arg)]n-Asp is the substrate for the second reaction catalysed by cyanophycin synthase, EC 6.3.2.30 ?
6.3.2.30 ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg Trichormus variabilis a small amount of cyanophycin is required as a primer ADP + phosphate + [L-Asp(4-L-Arg)]n+1 no formation of AMP, [L-Asp(4-L-Arg)]n-Asp is the substrate for the second reaction catalysed by cyanophycin synthase ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.29 Trichormus variabilis ATCC 29413
-
-
-
6.3.2.30 Trichormus variabilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.29
-
 Trichormus variabilis ATCC 29413
6.3.2.30
-
 Trichormus variabilis

Reaction

EC Number Reaction Comment Organism Reaction ID
6.3.2.29 ATP + [L-Asp(4-L-Arg)]n + L-Asp = ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp i.e. the second reaction of cyanophycin synthesis catalysed by cyanophycin synthase, the first reaction is catalysed by the enzyme's other active centre Trichormus variabilis ATCC 29413
a
6.3.2.30 ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg = ADP + phosphate + [L-Asp(4-L-Arg)]n+1 i.e. the first reaction of cyanophycin synthesis, the second reaction is catalysed by activity EC 6.3.2.29, sequence analysis suggests that cyanophycin synthase has two ATP binding sites corresponding to two active sites Trichormus variabilis
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.29 ATP + [L-Asp(4-L-Arg)]3-L-Asp + L-Arg
-
 Trichormus variabilis ATCC 29413 ADP + phosphate + [L-Asp(4-L-Arg)]4 + L-Asp
-
 ?
6.3.2.29 ATP + [L-Asp(4-L-Arg)]n + L-Asp [L-Asp(4-L-Arg)]n-Asp is a cyanophycin molecule with a C-terminal L-Asp residue that is not linked to an L-Arg residue via its beta-carboxy group, this intermediate is produced in the first reaction catalysed by cyanophycin synthase Trichormus variabilis ATCC 29413 ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp no formation of AMP ?
6.3.2.29 L-aspartic acid + ATP
-
 Trichormus variabilis ATCC 29413 poly-L-aspartic acid + ADP + phosphate
-
 ?
6.3.2.29 additional information no product is formed if the peptide primer is blocked at the C-terminus or if L-arginine is solely supplied as substrate Trichormus variabilis ATCC 29413 ?
-
 ?
6.3.2.29 [L-Asp(4-L-Arg)]n + L-Asp + ATP a small amount of cyanophycin is required as a primer Trichormus variabilis ATCC 29413 [L-Asp(4-L-Arg)]n-Asp + ADP + phosphate no formation of AMP, [L-Asp(4-L-Arg)]n-Asp is the substrate for the second reaction catalysed by cyanophycin synthase, EC 6.3.2.30 ?
6.3.2.30 ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg a small amount of cyanophycin is required as a primer Trichormus variabilis ADP + phosphate + [L-Asp(4-L-Arg)]n+1 no formation of AMP, [L-Asp(4-L-Arg)]n-Asp is the substrate for the second reaction catalysed by cyanophycin synthase ?
6.3.2.30 ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg a short peptide is required as a primer, the primer peptide is elongated at its C-terminus Trichormus variabilis ADP + phosphate + [L-Asp(4-L-Arg)]n+1
-
 ?
6.3.2.30 L-aspartic acid + ATP
-
 Trichormus variabilis poly-L-aspartic acid + ADP + phosphate
-
 ?
6.3.2.30 additional information no product is formed if the peptide primer is blocked at the C-terminus or if L-arginine is solely supplied as substrate Trichormus variabilis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.2.29 CphA
-
 Trichormus variabilis ATCC 29413
6.3.2.29 cyanophycin synthetase
-
 Trichormus variabilis ATCC 29413
6.3.2.30 CphA
-
 Trichormus variabilis
6.3.2.30 cyanophycin synthetase
-
 Trichormus variabilis