EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
6.3.2.29 | K+ | - |
Trichormus variabilis | |
6.3.2.29 | Mg2+ | - |
Trichormus variabilis | |
6.3.2.29 | sulfhydryl reagents | - |
Trichormus variabilis | |
6.3.2.30 | K+ | - |
Trichormus variabilis | |
6.3.2.30 | Mg2+ | - |
Trichormus variabilis | |
6.3.2.30 | sulfhydryl reagents | - |
Trichormus variabilis |
EC Number | Cloned (Comment) | Organism |
---|---|---|
6.3.2.29 | expression in Escherichia coli DH5alpha | Trichormus variabilis |
6.3.2.30 | expression in Escherichia coli DH5alpha | Trichormus variabilis |
EC Number | General Stability | Organism |
---|---|---|
6.3.2.29 | purified enzyme is unstable at both 0°C and -70°C | Trichormus variabilis |
6.3.2.30 | purified enzyme is unstable at both 0°C and -70°C | Trichormus variabilis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
6.3.2.29 | Mg2+ | as Mg-ATP | Trichormus variabilis | |
6.3.2.30 | Mg2+ | as Mg-ATP | Trichormus variabilis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
6.3.2.29 | 100000 | - |
2 * 100000, SDS-PAGE | Trichormus variabilis |
6.3.2.29 | 230000 | - |
gel filtration | Trichormus variabilis |
6.3.2.30 | 100000 | - |
2 * 100000, SDS-PAGE | Trichormus variabilis |
6.3.2.30 | 230000 | - |
gel filtration | Trichormus variabilis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.3.2.29 | Trichormus variabilis | O86109 | - |
- |
6.3.2.30 | Trichormus variabilis | O86109 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.3.2.29 | dye-ligand, gel filtration and ion-exchange chromatography, enriched about 4500fold | Trichormus variabilis |
6.3.2.30 | dye-ligand, gel filtration and ion-exchange chromatography, enriched about 4500fold | Trichormus variabilis |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
6.3.2.29 | ATP + [L-Asp(4-L-Arg)]n + L-Asp = ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp | i.e. the second reaction of cyanophycin synthesis catalysed by cyanophycin synthase, the first reaction is catalysed by the enzyme's other active centre | Trichormus variabilis | |
6.3.2.30 | ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg = ADP + phosphate + [L-Asp(4-L-Arg)]n+1 | i.e. the first reaction of cyanophycin synthesis, the second reaction is catalysed by the enzyme's other active centre | Trichormus variabilis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
6.3.2.29 | 0.00221 | - |
at 28°C, pH 8.2 | Trichormus variabilis |
6.3.2.30 | 0.00221 | - |
at pH 8.2 and 28°C | Trichormus variabilis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.2.29 | ATP + [L-Asp(4-L-Arg)]n + L-Asp | [L-Asp(4-L-Arg)]n-Asp is a cyanophycin molecule with a C-terminal L-Asp residue that is not linked to an L-Arg residue via its beta-carboxy group, this intermediate is produced in the first reaction catalysed by cyanophycin synthase | Trichormus variabilis | ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp | no formation of AMP | ? | |
6.3.2.30 | ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg | a small amount of cyanophycin is required as a primer | Trichormus variabilis | ADP + phosphate + [L-Asp(4-L-Arg)]n+1 | no formation of AMP, [L-Asp(4-L-Arg)]n-Asp is the substrate for the second reaction catalysed by cyanophycin synthase, EC 6.3.2.30 | ? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.3.2.29 | dimer | 2 * 100000, SDS-PAGE | Trichormus variabilis |
6.3.2.30 | dimer | 2 * 100000, SDS-PAGE | Trichormus variabilis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.3.2.29 | cyanophycin synthetase | - |
Trichormus variabilis |
6.3.2.30 | cyanophycin synthetase | - |
Trichormus variabilis |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.3.2.30 | additional information | - |
purified enzyme is unstable at both 0°C and -70°C | Trichormus variabilis |