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Literature summary extracted from

  • Okuyama, M.; Tanimoto, Y.; Ito, T.; Anzai, A.; Mori, H.; Kimura, A.; Matsui, H.; Chiba, S.
    Purification and characterization of the hyper-glycosylated extracellular alpha-glucosidase from Schizosaccharomyces pombe (2005), Enzyme Microb. Technol., 37, 472-480.
No PubMed abstract available

General Stability

EC Number General Stability Organism
3.2.1.20 proteolysis susceptibility is descended by enzymatical deglycosylation Schizosaccharomyces pombe

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.20 additional information
-
 additional information kinetics Schizosaccharomyces pombe
3.2.1.20 4.9
-
 phenyl alpha-glucoside pH 4.5, 35°C Schizosaccharomyces pombe
3.2.1.20 6.3
-
 maltose pH 4.5, 35°C Schizosaccharomyces pombe
3.2.1.20 6.7
-
 maltotriose pH 4.5, 35°C Schizosaccharomyces pombe
3.2.1.20 7.1
-
 maltotetraose pH 4.5, 35°C Schizosaccharomyces pombe
3.2.1.20 9.9
-
 Phenyl alpha-maltoside pH 4.5, 35°C Schizosaccharomyces pombe
3.2.1.20 14
-
 maltopentaose pH 4.5, 35°C Schizosaccharomyces pombe
3.2.1.20 14
-
 isomaltose pH 4.5, 35°C Schizosaccharomyces pombe
3.2.1.20 19
-
 kojibiose pH 4.5, 35°C Schizosaccharomyces pombe
3.2.1.20 43
-
 maltohexaose pH 4.5, 35°C Schizosaccharomyces pombe
3.2.1.20 55
-
 nigerose pH 4.5, 35°C Schizosaccharomyces pombe
3.2.1.20 62
-
 panose pH 4.5, 35°C Schizosaccharomyces pombe
3.2.1.20 76
-
 maltoheptaose pH 4.5, 35°C Schizosaccharomyces pombe

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.20 extracellular
-
 Schizosaccharomyces pombe
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.20 106139
-
 1 * 110000, deglycosylated enzyme, SDS-PAGE, 1 * 106139, amino acid sequence calculation Schizosaccharomyces pombe
3.2.1.20 1120000
-
-
 Schizosaccharomyces pombe

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.20 maltose + H2O Schizosaccharomyces pombe
-
 2 alpha-D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.20 Schizosaccharomyces pombe Q9C0Y4
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.20 glycoprotein the extracellular enzyme is hyperglycosylated and contains 88% carbohydrate, mainly N-glycosylation, overview, deglycosylation decreases the heat stability and susceptibility to proteolysis Schizosaccharomyces pombe

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.20 native enzyme from culture supernatant by ethanol precipitation, anion exchange chromatography, and gel filtration Schizosaccharomyces pombe

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.20 maltotetraose + 3 H2O = 4 alpha-D-glucose the enzyme prefers short substrates, e.g. maltose and maltotriose, to longer substrates, and hydrolyzes alpha-1,4-glucosidic linkages, but also acts on alpha-1,2-, alpha-1,3-, and alpha-1,6-glucosidic linkages, the catalytic site contains three subsites with different affinities Schizosaccharomyces pombe
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.20 culture supernatant
-
 Schizosaccharomyces pombe
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.20 2 2.5 purified enzyme Schizosaccharomyces pombe

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.20 isomaltose + H2O low activity Schizosaccharomyces pombe 2 alpha-D-glucose
-
 ?
3.2.1.20 kojibiose + H2O low activity Schizosaccharomyces pombe alpha-D-glucose + D-glucose
-
 ?
3.2.1.20 maltoheptaose + 6 H2O low activity Schizosaccharomyces pombe 7 alpha-D-glucose
-
 ?
3.2.1.20 maltohexaose + 5 H2O low activity Schizosaccharomyces pombe 6 alpha-D-glucose
-
 ?
3.2.1.20 maltopentaose + 4 H2O
-
 Schizosaccharomyces pombe 5 alpha-D-glucose
-
 ?
3.2.1.20 maltose + H2O
-
 Schizosaccharomyces pombe 2 alpha-D-glucose
-
 ?
3.2.1.20 maltotetraose + 3 H2O
-
 Schizosaccharomyces pombe 4 alpha-D-glucose
-
 ?
3.2.1.20 maltotriose + 2 H2O
-
 Schizosaccharomyces pombe 3 alpha-D-glucose
-
 ?
3.2.1.20 additional information the enzyme prefers short substrates, e.g. maltose and maltotriose, to longer substrates, and hydrolyzes alpha-1,4-glucosidic linkages, but also acts on alpha-1,2-, alpha-1,3-, and alpha-1,6-glucosidic linkages, substrate specificity, soluble starch is a poor substrate, the catalytic site contains three subsites, subsite affinity, overview Schizosaccharomyces pombe ?
-
 ?
3.2.1.20 nigerose + H2O alpha-1,3-linkage, very low activity Schizosaccharomyces pombe alpha-D-glucose + D-glucose
-
 ?
3.2.1.20 panose + H2O low activity Schizosaccharomyces pombe maltose + alpha-D-glucose
-
 ?
3.2.1.20 phenyl alpha-glucoside + H2O low activity Schizosaccharomyces pombe alpha-D-glucose + phenol
-
 ?
3.2.1.20 phenyl alpha-maltoside + H2O preferred substrate, stereospecific production of alpha-D-glucose which spontaneously mutarotates to beta-glucose Schizosaccharomyces pombe alpha-D-glucose + ?
-
 ?
3.2.1.20 soluble starch + H2O
-
 Schizosaccharomyces pombe alpha-D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.20 monomer 1 * 110000, deglycosylated enzyme, SDS-PAGE, 1 * 106139, amino acid sequence calculation Schizosaccharomyces pombe

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.20 35
-
 assay at Schizosaccharomyces pombe

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.20 additional information
-
 heat stability and is descended by enzymatical deglycosylation Schizosaccharomyces pombe
3.2.1.20 37
-
 deglycosylated enzyme is labile above Schizosaccharomyces pombe
3.2.1.20 40
-
 15 min, native enzyme is stable up to Schizosaccharomyces pombe
3.2.1.20 50
-
 15 min, inactivation Schizosaccharomyces pombe

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.20 111
-
 maltotetraose pH 4.5, 35°C Schizosaccharomyces pombe
3.2.1.20 139
-
 Phenyl alpha-maltoside pH 4.5, 35°C Schizosaccharomyces pombe
3.2.1.20 216
-
 isomaltose pH 4.5, 35°C Schizosaccharomyces pombe
3.2.1.20 433
-
 kojibiose pH 4.5, 35°C Schizosaccharomyces pombe
3.2.1.20 435
-
 nigerose pH 4.5, 35°C Schizosaccharomyces pombe
3.2.1.20 704
-
 panose pH 4.5, 35°C Schizosaccharomyces pombe
3.2.1.20 709
-
 maltose pH 4.5, 35°C Schizosaccharomyces pombe
3.2.1.20 810
-
 maltopentaose pH 4.5, 35°C Schizosaccharomyces pombe
3.2.1.20 874
-
 maltohexaose pH 4.5, 35°C Schizosaccharomyces pombe
3.2.1.20 994
-
 maltotriose pH 4.5, 35°C Schizosaccharomyces pombe
3.2.1.20 1020
-
 maltoheptaose pH 4.5, 35°C Schizosaccharomyces pombe
3.2.1.20 1171
-
 Phenyl alpha-maltoside pH 4.5, 35°C Schizosaccharomyces pombe
3.2.1.20 1171
-
 phenyl alpha-glucoside pH 4.5, 35°C Schizosaccharomyces pombe

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.20 4.5
-
-
 Schizosaccharomyces pombe

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.20 additional information
-
 pH-profile Schizosaccharomyces pombe

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.20 3 8 4°C, 24 h, native and deglycosylated enzyme, stable Schizosaccharomyces pombe