BRENDA - Enzyme Database show

A few amino acid substitutions can convert deoxyribonucleoside kinase specificity from pyrimidines to purines

Knecht, W.; Sandrini, M.P.; Johansson, K.; Eklund, H.; Munch-Petersen, B.; Piskur, J.; Embo J. 21, 1873-1880 (2002)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.7.1.145
expression of wild-type and mutant enzymes in Escherichia coli strain BL21 as C-terminally His8-tagged proteins
Drosophila melanogaster
Engineering
EC Number
Amino acid exchange
Commentary
Organism
2.7.1.145
A110D
site-directed mutagenesis, altered substrate specificity and kinetics, and highly reduced activity compared to the wild-type enzyme
Drosophila melanogaster
2.7.1.145
M88R
site-directed mutagenesis, altered substrate specificity and kinetics, and highly reduced activity compared to the wild-type enzyme
Drosophila melanogaster
2.7.1.145
M88R/A110D
site-directed mutagenesis, altered substrate specificity and kinetics, and highly reduced activity compared to the wild-type enzyme
Drosophila melanogaster
2.7.1.145
V84A
site-directed mutagenesis, altered substrate specificity and kinetics, and reduced activity compared to the wild-type enzyme
Drosophila melanogaster
2.7.1.145
V84A/M88R/A110D
site-directed mutagenesis, altered substrate specificity and kinetics, and highly reduced activity compared to the wild-type enzyme
Drosophila melanogaster
2.7.1.145
V84M
site-directed mutagenesis, altered substrate specificity and kinetics, and highly reduced activity compared to the wild-type enzyme
Drosophila melanogaster
2.7.1.145
V84S
site-directed mutagenesis, altered substrate specificity and kinetics, and reduced activity compared to the wild-type enzyme
Drosophila melanogaster
2.7.1.145
V84S/M88R/A110D
site-directed mutagenesis, altered substrate specificity and kinetics, and highly reduced activity compared to the wild-type enzyme
Drosophila melanogaster
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.145
0.0012
-
thymidine
recombinant wild-type enzyme
Drosophila melanogaster
2.7.1.145
0.0023
-
2'-deoxycytidine
recombinant wild-type enzyme
Drosophila melanogaster
2.7.1.145
0.0025
-
2'-deoxycytidine
recombinant mutant V84A
Drosophila melanogaster
2.7.1.145
0.0113
-
thymidine
recombinant mutant V84A
Drosophila melanogaster
2.7.1.145
0.0129
-
2'-deoxycytidine
recombinant mutant V84S
Drosophila melanogaster
2.7.1.145
0.0247
-
thymidine
recombinant mutant V84S
Drosophila melanogaster
2.7.1.145
0.04
-
2'-deoxyguanosine
recombinant mutant V84A/M88R/A110D
Drosophila melanogaster
2.7.1.145
0.064
-
thymidine
recombinant mutant A110D
Drosophila melanogaster
2.7.1.145
0.091
-
2'-deoxycytidine
recombinant mutant A110D
Drosophila melanogaster
2.7.1.145
0.147
-
2'-deoxyguanosine
recombinant mutant V84A
Drosophila melanogaster
2.7.1.145
0.15
-
2'-deoxyadenosine
recombinant mutant V84S
Drosophila melanogaster
2.7.1.145
0.153
-
2'-deoxyadenosine
recombinant mutant V84A/M88R/A110D
Drosophila melanogaster
2.7.1.145
0.159
-
2'-deoxyguanosine
recombinant mutant V84S/M88R/A110D
Drosophila melanogaster
2.7.1.145
0.225
-
2'-deoxyadenosine
recombinant wild-type enzyme
Drosophila melanogaster
2.7.1.145
0.237
-
thymidine
recombinant mutant M88R
Drosophila melanogaster
2.7.1.145
0.239
-
2'-deoxyadenosine
recombinant mutant A110D
Drosophila melanogaster
2.7.1.145
0.257
-
2'-deoxyguanosine
recombinant mutant A110D
Drosophila melanogaster
2.7.1.145
0.292
-
Dideoxycytidine
recombinant mutant V84A
Drosophila melanogaster
2.7.1.145
0.36
-
2'-deoxyadenosine
recombinant mutant M88R/A110D
Drosophila melanogaster
2.7.1.145
0.375
-
2'-deoxyguanosine
recombinant mutant V84S
Drosophila melanogaster
2.7.1.145
0.388
-
2'-deoxyadenosine
recombinant mutant V84S/M88R/A110D
Drosophila melanogaster
2.7.1.145
0.549
-
2'-deoxyguanosine
recombinant mutant M88R/A110D
Drosophila melanogaster
2.7.1.145
0.584
-
2'-deoxycytidine
recombinant mutant M88R/A110D
Drosophila melanogaster
2.7.1.145
0.627
-
2'-deoxycytidine
recombinant mutant V84A/M88R/A110D
Drosophila melanogaster
2.7.1.145
0.645
-
2'-deoxyguanosine
recombinant mutant M88R
Drosophila melanogaster
2.7.1.145
0.665
-
2'-deoxyguanosine
recombinant wild-type enzyme
Drosophila melanogaster
2.7.1.145
0.674
-
2'-deoxycytidine
recombinant mutant V84M
Drosophila melanogaster
2.7.1.145
0.7
-
2'-deoxyadenosine
recombinant mutant V84A
Drosophila melanogaster
2.7.1.145
0.829
-
thymidine
recombinant mutant V84M
Drosophila melanogaster
2.7.1.145
0.852
-
2'-deoxyadenosine
recombinant mutant V84M
Drosophila melanogaster
2.7.1.145
1.039
-
2'-deoxycytidine
recombinant mutant M88R
Drosophila melanogaster
2.7.1.145
1.124
-
Dideoxycytidine
recombinant wild-type enzyme
Drosophila melanogaster
2.7.1.145
1.512
-
thymidine
recombinant mutant V84A/M88R/A110D
Drosophila melanogaster
2.7.1.145
1.911
-
2'-deoxyguanosine
recombinant mutant V84M
Drosophila melanogaster
2.7.1.145
2.088
-
2'-deoxyadenosine
recombinant mutant M88R
Drosophila melanogaster
2.7.1.145
2.162
-
2'-deoxycytidine
recombinant mutant V84S/M88R/A110D
Drosophila melanogaster
2.7.1.145
2.425
-
thymidine
recombinant mutant M88R/A110D
Drosophila melanogaster
2.7.1.145
6.56
-
thymidine
recombinant mutant V84S/M88R/A110D
Drosophila melanogaster
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.145
Mg2+
-
Drosophila melanogaster
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.145
additional information
Drosophila melanogaster
enzyme evolution by gene duplication of a progenitor kinase
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.145
Drosophila melanogaster
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.145
recombinant C-terminally His8-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 by nickel affinity chromatography
Drosophila melanogaster
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.145
additional information
-
catalytic efficiencies of wild-type and mutant enzymes, overview
Drosophila melanogaster
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.145
ATP + 2'-deoxyadenosine
-
661583
Drosophila melanogaster
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxycytidine
wild-type enzyme: second-best substrate
661583
Drosophila melanogaster
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxyguanosine
-
661583
Drosophila melanogaster
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + dideoxycytidine
wild-type enzyme: low activity
661583
Drosophila melanogaster
ADP + dideoxycytidine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + thymidine
wild-type enzyme: preferred substrate
661583
Drosophila melanogaster
ADP + thymidine 5'-phosphate
-
-
-
?
2.7.1.145
additional information
enzyme evolution by gene duplication of a progenitor kinase
661583
Drosophila melanogaster
?
-
-
-
-
2.7.1.145
additional information
amino acid residues responsible for substrate specificity are V84, M88, and A110, wild-type enzyme shows broad substrate specificity preferring pyrimidines to purines, substrate specificities of wild-type and mutant enzymes, overview
661583
Drosophila melanogaster
?
-
-
-
-
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.1.145
0.034
-
thymidine
recombinant mutant M88R/A110D
Drosophila melanogaster
2.7.1.145
0.037
-
thymidine
recombinant mutant V84M
Drosophila melanogaster
2.7.1.145
0.073
-
2'-deoxyguanosine
recombinant mutant A110D
Drosophila melanogaster
2.7.1.145
0.084
-
2'-deoxyadenosine
recombinant mutant A110D
Drosophila melanogaster
2.7.1.145
0.11
-
thymidine
recombinant mutant V84A/M88R/A110D
Drosophila melanogaster
2.7.1.145
0.13
-
thymidine
recombinant mutant M88R
Drosophila melanogaster
2.7.1.145
0.22
-
2'-deoxyguanosine
recombinant mutant V84M
Drosophila melanogaster
2.7.1.145
0.23
-
thymidine
recombinant mutant V84S/M88R/A110D
Drosophila melanogaster
2.7.1.145
0.27
-
2'-deoxycytidine
recombinant mutant A110D
Drosophila melanogaster
2.7.1.145
0.35
-
thymidine
recombinant mutant A110D
Drosophila melanogaster
2.7.1.145
0.64
-
2'-deoxyguanosine
recombinant mutant V84S
Drosophila melanogaster
2.7.1.145
0.83
-
2'-deoxycytidine
recombinant mutant V84S
Drosophila melanogaster
2.7.1.145
0.83
-
thymidine
recombinant mutant V84S
Drosophila melanogaster
2.7.1.145
0.93
-
Dideoxycytidine
recombinant mutant V84A
Drosophila melanogaster
2.7.1.145
1.1
-
2'-deoxyadenosine
recombinant mutant V84S
Drosophila melanogaster
2.7.1.145
1.36
-
2'-deoxyadenosine
recombinant mutant V84M
Drosophila melanogaster
2.7.1.145
1.62
-
2'-deoxycytidine
recombinant mutant V84S/M88R/A110D
Drosophila melanogaster
2.7.1.145
1.73
-
2'-deoxyguanosine
recombinant mutant V84A/M88R/A110D
Drosophila melanogaster
2.7.1.145
1.8
-
2'-deoxycytidine
recombinant mutant V84M
Drosophila melanogaster
2.7.1.145
1.88
-
2'-deoxyadenosine
recombinant mutant M88R
Drosophila melanogaster
2.7.1.145
1.94
-
2'-deoxycytidine
recombinant mutant V84A/M88R/A110D
Drosophila melanogaster
2.7.1.145
2.7
-
2'-deoxyguanosine
recombinant mutant M88R
Drosophila melanogaster
2.7.1.145
2.8
-
2'-deoxyguanosine
recombinant mutant V84A
Drosophila melanogaster
2.7.1.145
2.97
-
thymidine
recombinant mutant V84A
Drosophila melanogaster
2.7.1.145
3.2
-
2'-deoxyadenosine
recombinant mutant V84S/M88R/A110D
Drosophila melanogaster
2.7.1.145
3.5
-
2'-deoxyadenosine
recombinant mutant V84A/M88R/A110D
Drosophila melanogaster
2.7.1.145
3.5
-
2'-deoxycytidine
recombinant mutant M88R/A110D
Drosophila melanogaster
2.7.1.145
3.7
-
2'-deoxyadenosine
recombinant mutant V84A
Drosophila melanogaster
2.7.1.145
3.7
-
2'-deoxyguanosine
recombinant mutant M88R/A110D
Drosophila melanogaster
2.7.1.145
4.2
-
Dideoxycytidine
recombinant wild-type enzyme
Drosophila melanogaster
2.7.1.145
4.4
-
2'-deoxycytidine
recombinant mutant V84A
Drosophila melanogaster
2.7.1.145
4.6
-
2'-deoxyguanosine
recombinant mutant V84S/M88R/A110D
Drosophila melanogaster
2.7.1.145
6.9
-
2'-deoxyadenosine
recombinant mutant M88R/A110D
Drosophila melanogaster
2.7.1.145
7.5
-
2'-deoxycytidine
recombinant mutant M88R
Drosophila melanogaster
2.7.1.145
14.2
-
thymidine
recombinant wild-type enzyme
Drosophila melanogaster
2.7.1.145
15.1
-
2'-deoxyguanosine
recombinant wild-type enzyme
Drosophila melanogaster
2.7.1.145
16.5
-
2'-deoxycytidine
recombinant wild-type enzyme
Drosophila melanogaster
2.7.1.145
20.6
-
2'-deoxyadenosine
recombinant wild-type enzyme
Drosophila melanogaster
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.145
ATP
-
Drosophila melanogaster
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.145
expression of wild-type and mutant enzymes in Escherichia coli strain BL21 as C-terminally His8-tagged proteins
Drosophila melanogaster
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.145
ATP
-
Drosophila melanogaster
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
2.7.1.145
A110D
site-directed mutagenesis, altered substrate specificity and kinetics, and highly reduced activity compared to the wild-type enzyme
Drosophila melanogaster
2.7.1.145
M88R
site-directed mutagenesis, altered substrate specificity and kinetics, and highly reduced activity compared to the wild-type enzyme
Drosophila melanogaster
2.7.1.145
M88R/A110D
site-directed mutagenesis, altered substrate specificity and kinetics, and highly reduced activity compared to the wild-type enzyme
Drosophila melanogaster
2.7.1.145
V84A
site-directed mutagenesis, altered substrate specificity and kinetics, and reduced activity compared to the wild-type enzyme
Drosophila melanogaster
2.7.1.145
V84A/M88R/A110D
site-directed mutagenesis, altered substrate specificity and kinetics, and highly reduced activity compared to the wild-type enzyme
Drosophila melanogaster
2.7.1.145
V84M
site-directed mutagenesis, altered substrate specificity and kinetics, and highly reduced activity compared to the wild-type enzyme
Drosophila melanogaster
2.7.1.145
V84S
site-directed mutagenesis, altered substrate specificity and kinetics, and reduced activity compared to the wild-type enzyme
Drosophila melanogaster
2.7.1.145
V84S/M88R/A110D
site-directed mutagenesis, altered substrate specificity and kinetics, and highly reduced activity compared to the wild-type enzyme
Drosophila melanogaster
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.145
0.0012
-
thymidine
recombinant wild-type enzyme
Drosophila melanogaster
2.7.1.145
0.0023
-
2'-deoxycytidine
recombinant wild-type enzyme
Drosophila melanogaster
2.7.1.145
0.0025
-
2'-deoxycytidine
recombinant mutant V84A
Drosophila melanogaster
2.7.1.145
0.0113
-
thymidine
recombinant mutant V84A
Drosophila melanogaster
2.7.1.145
0.0129
-
2'-deoxycytidine
recombinant mutant V84S
Drosophila melanogaster
2.7.1.145
0.0247
-
thymidine
recombinant mutant V84S
Drosophila melanogaster
2.7.1.145
0.04
-
2'-deoxyguanosine
recombinant mutant V84A/M88R/A110D
Drosophila melanogaster
2.7.1.145
0.064
-
thymidine
recombinant mutant A110D
Drosophila melanogaster
2.7.1.145
0.091
-
2'-deoxycytidine
recombinant mutant A110D
Drosophila melanogaster
2.7.1.145
0.147
-
2'-deoxyguanosine
recombinant mutant V84A
Drosophila melanogaster
2.7.1.145
0.15
-
2'-deoxyadenosine
recombinant mutant V84S
Drosophila melanogaster
2.7.1.145
0.153
-
2'-deoxyadenosine
recombinant mutant V84A/M88R/A110D
Drosophila melanogaster
2.7.1.145
0.159
-
2'-deoxyguanosine
recombinant mutant V84S/M88R/A110D
Drosophila melanogaster
2.7.1.145
0.225
-
2'-deoxyadenosine
recombinant wild-type enzyme
Drosophila melanogaster
2.7.1.145
0.237
-
thymidine
recombinant mutant M88R
Drosophila melanogaster
2.7.1.145
0.239
-
2'-deoxyadenosine
recombinant mutant A110D
Drosophila melanogaster
2.7.1.145
0.257
-
2'-deoxyguanosine
recombinant mutant A110D
Drosophila melanogaster
2.7.1.145
0.292
-
Dideoxycytidine
recombinant mutant V84A
Drosophila melanogaster
2.7.1.145
0.36
-
2'-deoxyadenosine
recombinant mutant M88R/A110D
Drosophila melanogaster
2.7.1.145
0.375
-
2'-deoxyguanosine
recombinant mutant V84S
Drosophila melanogaster
2.7.1.145
0.388
-
2'-deoxyadenosine
recombinant mutant V84S/M88R/A110D
Drosophila melanogaster
2.7.1.145
0.549
-
2'-deoxyguanosine
recombinant mutant M88R/A110D
Drosophila melanogaster
2.7.1.145
0.584
-
2'-deoxycytidine
recombinant mutant M88R/A110D
Drosophila melanogaster
2.7.1.145
0.627
-
2'-deoxycytidine
recombinant mutant V84A/M88R/A110D
Drosophila melanogaster
2.7.1.145
0.645
-
2'-deoxyguanosine
recombinant mutant M88R
Drosophila melanogaster
2.7.1.145
0.665
-
2'-deoxyguanosine
recombinant wild-type enzyme
Drosophila melanogaster
2.7.1.145
0.674
-
2'-deoxycytidine
recombinant mutant V84M
Drosophila melanogaster
2.7.1.145
0.7
-
2'-deoxyadenosine
recombinant mutant V84A
Drosophila melanogaster
2.7.1.145
0.829
-
thymidine
recombinant mutant V84M
Drosophila melanogaster
2.7.1.145
0.852
-
2'-deoxyadenosine
recombinant mutant V84M
Drosophila melanogaster
2.7.1.145
1.039
-
2'-deoxycytidine
recombinant mutant M88R
Drosophila melanogaster
2.7.1.145
1.124
-
Dideoxycytidine
recombinant wild-type enzyme
Drosophila melanogaster
2.7.1.145
1.512
-
thymidine
recombinant mutant V84A/M88R/A110D
Drosophila melanogaster
2.7.1.145
1.911
-
2'-deoxyguanosine
recombinant mutant V84M
Drosophila melanogaster
2.7.1.145
2.088
-
2'-deoxyadenosine
recombinant mutant M88R
Drosophila melanogaster
2.7.1.145
2.162
-
2'-deoxycytidine
recombinant mutant V84S/M88R/A110D
Drosophila melanogaster
2.7.1.145
2.425
-
thymidine
recombinant mutant M88R/A110D
Drosophila melanogaster
2.7.1.145
6.56
-
thymidine
recombinant mutant V84S/M88R/A110D
Drosophila melanogaster
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.145
Mg2+
-
Drosophila melanogaster
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.145
additional information
Drosophila melanogaster
enzyme evolution by gene duplication of a progenitor kinase
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.145
recombinant C-terminally His8-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 by nickel affinity chromatography
Drosophila melanogaster
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.145
additional information
-
catalytic efficiencies of wild-type and mutant enzymes, overview
Drosophila melanogaster
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.145
ATP + 2'-deoxyadenosine
-
661583
Drosophila melanogaster
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxycytidine
wild-type enzyme: second-best substrate
661583
Drosophila melanogaster
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxyguanosine
-
661583
Drosophila melanogaster
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + dideoxycytidine
wild-type enzyme: low activity
661583
Drosophila melanogaster
ADP + dideoxycytidine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + thymidine
wild-type enzyme: preferred substrate
661583
Drosophila melanogaster
ADP + thymidine 5'-phosphate
-
-
-
?
2.7.1.145
additional information
enzyme evolution by gene duplication of a progenitor kinase
661583
Drosophila melanogaster
?
-
-
-
-
2.7.1.145
additional information
amino acid residues responsible for substrate specificity are V84, M88, and A110, wild-type enzyme shows broad substrate specificity preferring pyrimidines to purines, substrate specificities of wild-type and mutant enzymes, overview
661583
Drosophila melanogaster
?
-
-
-
-
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.1.145
0.034
-
thymidine
recombinant mutant M88R/A110D
Drosophila melanogaster
2.7.1.145
0.037
-
thymidine
recombinant mutant V84M
Drosophila melanogaster
2.7.1.145
0.073
-
2'-deoxyguanosine
recombinant mutant A110D
Drosophila melanogaster
2.7.1.145
0.084
-
2'-deoxyadenosine
recombinant mutant A110D
Drosophila melanogaster
2.7.1.145
0.11
-
thymidine
recombinant mutant V84A/M88R/A110D
Drosophila melanogaster
2.7.1.145
0.13
-
thymidine
recombinant mutant M88R
Drosophila melanogaster
2.7.1.145
0.22
-
2'-deoxyguanosine
recombinant mutant V84M
Drosophila melanogaster
2.7.1.145
0.23
-
thymidine
recombinant mutant V84S/M88R/A110D
Drosophila melanogaster
2.7.1.145
0.27
-
2'-deoxycytidine
recombinant mutant A110D
Drosophila melanogaster
2.7.1.145
0.35
-
thymidine
recombinant mutant A110D
Drosophila melanogaster
2.7.1.145
0.64
-
2'-deoxyguanosine
recombinant mutant V84S
Drosophila melanogaster
2.7.1.145
0.83
-
2'-deoxycytidine
recombinant mutant V84S
Drosophila melanogaster
2.7.1.145
0.83
-
thymidine
recombinant mutant V84S
Drosophila melanogaster
2.7.1.145
0.93
-
Dideoxycytidine
recombinant mutant V84A
Drosophila melanogaster
2.7.1.145
1.1
-
2'-deoxyadenosine
recombinant mutant V84S
Drosophila melanogaster
2.7.1.145
1.36
-
2'-deoxyadenosine
recombinant mutant V84M
Drosophila melanogaster
2.7.1.145
1.62
-
2'-deoxycytidine
recombinant mutant V84S/M88R/A110D
Drosophila melanogaster
2.7.1.145
1.73
-
2'-deoxyguanosine
recombinant mutant V84A/M88R/A110D
Drosophila melanogaster
2.7.1.145
1.8
-
2'-deoxycytidine
recombinant mutant V84M
Drosophila melanogaster
2.7.1.145
1.88
-
2'-deoxyadenosine
recombinant mutant M88R
Drosophila melanogaster
2.7.1.145
1.94
-
2'-deoxycytidine
recombinant mutant V84A/M88R/A110D
Drosophila melanogaster
2.7.1.145
2.7
-
2'-deoxyguanosine
recombinant mutant M88R
Drosophila melanogaster
2.7.1.145
2.8
-
2'-deoxyguanosine
recombinant mutant V84A
Drosophila melanogaster
2.7.1.145
2.97
-
thymidine
recombinant mutant V84A
Drosophila melanogaster
2.7.1.145
3.2
-
2'-deoxyadenosine
recombinant mutant V84S/M88R/A110D
Drosophila melanogaster
2.7.1.145
3.5
-
2'-deoxyadenosine
recombinant mutant V84A/M88R/A110D
Drosophila melanogaster
2.7.1.145
3.5
-
2'-deoxycytidine
recombinant mutant M88R/A110D
Drosophila melanogaster
2.7.1.145
3.7
-
2'-deoxyadenosine
recombinant mutant V84A
Drosophila melanogaster
2.7.1.145
3.7
-
2'-deoxyguanosine
recombinant mutant M88R/A110D
Drosophila melanogaster
2.7.1.145
4.2
-
Dideoxycytidine
recombinant wild-type enzyme
Drosophila melanogaster
2.7.1.145
4.4
-
2'-deoxycytidine
recombinant mutant V84A
Drosophila melanogaster
2.7.1.145
4.6
-
2'-deoxyguanosine
recombinant mutant V84S/M88R/A110D
Drosophila melanogaster
2.7.1.145
6.9
-
2'-deoxyadenosine
recombinant mutant M88R/A110D
Drosophila melanogaster
2.7.1.145
7.5
-
2'-deoxycytidine
recombinant mutant M88R
Drosophila melanogaster
2.7.1.145
14.2
-
thymidine
recombinant wild-type enzyme
Drosophila melanogaster
2.7.1.145
15.1
-
2'-deoxyguanosine
recombinant wild-type enzyme
Drosophila melanogaster
2.7.1.145
16.5
-
2'-deoxycytidine
recombinant wild-type enzyme
Drosophila melanogaster
2.7.1.145
20.6
-
2'-deoxyadenosine
recombinant wild-type enzyme
Drosophila melanogaster