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Literature summary extracted from

  • Murata, D.; Endo, Y.; Obata, T.; Sakamoto, K.; Syouji, Y.; Kadohira, M.; Matsuda, A.; Sasaki, T.
    A crucial role of uridine/cytidine kinase 2 in antitumor activity of 3'-ethynyl nucleosides (2004), Drug Metab. Dispos., 32, 1178-1182.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.48 expressed as His-tag fusion protein in Escherichia coli BL21 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.48 R162W no activity Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.48 29000
-
 1 * 31000, UCK1, SDS-PAGE, 1 * 29000, UCK2, SDS-PAGE Homo sapiens
2.7.1.48 31000
-
 1 * 31000, UCK1, SDS-PAGE, 1 * 29000, UCK2, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.48 ATP + 1-(3-C-ethynyl-beta-D-ribopentofuranosyl)cytosine Homo sapiens UCK2 enzyme activity required to produce the antitumoral active metabolite ADP + 1-(3-C-ethynyl-beta-D-ribopentofuranosyl)cytosine 5'-phosphate
-
 ?
2.7.1.48 ATP + 1-(3-C-ethynyl-beta-D-ribopentofuranosyl)uridine Homo sapiens UCK2 enzyme activity required to produce the antitumoral active metabolite ADP + 1-(3-C-ethynyl-beta-D-ribopentofuranosyl)uridine 5'-phosphate
-
 ?
2.7.1.48 ATP + cytidine Homo sapiens rate limiting enzyme of the pyrimidine-nucleotide salvage pathway ADP + CMP
-
 ?
2.7.1.48 ATP + uridine Homo sapiens rate limiting enzyme of the pyrimidine-nucleotide salvage pathway ADP + UMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.48 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.48 recombinant protein using His-tag Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.48 HT-1080 cell fibrosarcoma cell line highly resistant to 3’-ethynyl nucleosides Homo sapiens
-
2.7.1.48 NUGC-3 cell gastric carcinoma cell line highly resistant to 3’-ethynyl nucleosides Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.48 ATP + 1-(3-C-ethynyl-beta-D-ribopentofuranosyl)cytosine
-
 Homo sapiens ADP + 1-(3-C-ethynyl-beta-D-ribopentofuranosyl)cytosine 5'-phosphate
-
 ?
2.7.1.48 ATP + 1-(3-C-ethynyl-beta-D-ribopentofuranosyl)cytosine UCK2 enzyme activity required to produce the antitumoral active metabolite Homo sapiens ADP + 1-(3-C-ethynyl-beta-D-ribopentofuranosyl)cytosine 5'-phosphate
-
 ?
2.7.1.48 ATP + 1-(3-C-ethynyl-beta-D-ribopentofuranosyl)uridine
-
 Homo sapiens ADP + 1-(3-C-ethynyl-beta-D-ribopentofuranosyl)uridine 5'-phosphate
-
 ?
2.7.1.48 ATP + 1-(3-C-ethynyl-beta-D-ribopentofuranosyl)uridine UCK2 enzyme activity required to produce the antitumoral active metabolite Homo sapiens ADP + 1-(3-C-ethynyl-beta-D-ribopentofuranosyl)uridine 5'-phosphate
-
 ?
2.7.1.48 ATP + cytidine
-
 Homo sapiens ADP + CMP
-
 ?
2.7.1.48 ATP + cytidine rate limiting enzyme of the pyrimidine-nucleotide salvage pathway Homo sapiens ADP + CMP
-
 ?
2.7.1.48 ATP + uridine
-
 Homo sapiens ADP + UMP
-
 ?
2.7.1.48 ATP + uridine rate limiting enzyme of the pyrimidine-nucleotide salvage pathway Homo sapiens ADP + UMP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.48 ? 1 * 31000, UCK1, SDS-PAGE, 1 * 29000, UCK2, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.7.1.48 More two different enzymes termed UCK1 and UCK2 with 68.8% identity in the deduced amino acid sequence, increased expression of both forms in many tumor cells Homo sapiens