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Literature summary extracted from

  • Tsai, W.B.; Zhang, X.; Sharma, D.; Wu, W.; Kinsey, W.H.
    Role of Yes kinase during early zebrafish development (2005), Dev. Biol., 277, 129-141.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.10.2 expression of c-Yes domains U and SH3 as GST-fusion proteins in Escherichia coli strain DH5alpha Danio rerio

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.10.2 4-amino-5-(4-chlorophenyl)-7-(tert-butyl)pyrazolo[3,4-d] pyrimidine i.e. pp2, inhibits Src family PTKs, inhibition of zebrafish egg/embryo development by Yes kinase inhibition Danio rerio
2.7.10.2 4-amino-7-phenylpyrazolo[3,4-d] pyrimidine i.e. pp3, inhibits Src family PTKs, slight inhibition of zebrafish egg/embryo development by Yes kinase inhibition Danio rerio

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.10.2 plasma membrane
-
 Danio rerio 5886
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.10.2 Mg2+
-
 Danio rerio
2.7.10.2 Mn2+
-
 Danio rerio

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.10.2 62000
-
 x * 62000, recombinant Yes-kinase, SDS-PAGE Danio rerio

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.10.2 ATP + [FAK]-L-tyrosine Danio rerio activation of FAK during epiboly and gastrulation by dynamic phosphorylation at Tyr861 ADP + [FAK]-L-tyrosine phosphate
-
 ?
2.7.10.2 additional information Danio rerio Yes kinase is required during development, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.10.2 Danio rerio A1A5H8 zebrafish
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.10.2 phosphoprotein the Yes kinase performs autophosphorylation Danio rerio

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.10.2 recombinant c-Yes GST-fusion U and SH3 domains from Escherichia coli strain DH5alpha by glutathione affinity chromatography Danio rerio

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.10.2 egg
-
 Danio rerio
-
2.7.10.2 embryo
-
 Danio rerio
-
2.7.10.2 additional information enzyme is active at all stages of egg and embryo development Danio rerio
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.10.2 ATP + [FAK]-L-tyrosine
-
 Danio rerio ADP + [FAK]-L-tyrosine phosphate
-
 ?
2.7.10.2 ATP + [FAK]-L-tyrosine activation of FAK during epiboly and gastrulation by dynamic phosphorylation at Tyr861 Danio rerio ADP + [FAK]-L-tyrosine phosphate
-
 ?
2.7.10.2 additional information Yes kinase is required during development, overview Danio rerio ?
-
 ?
2.7.10.2 additional information the Yes kinase performs autophosphorylation Danio rerio ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.10.2 ? x * 62000, recombinant Yes-kinase, SDS-PAGE Danio rerio

Synonyms

EC Number Synonyms Comment Organism
2.7.10.2 More Yes kinase is a Src-family tyrosine kinase Danio rerio
2.7.10.2 Yes kinase
-
 Danio rerio

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.10.2 25
-
 assay at Danio rerio

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.10.2 7.2
-
 assay at Danio rerio

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.10.2 ATP
-
 Danio rerio