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Literature summary extracted from

  • Kawasaki, Y.; Onozuka, M.; Mizote, T.; Nosaka, K.
    Biosynthesis of hydroxymethylpyrimidine pyrophosphate in Saccharomyces cerevisiae (2005), Curr. Genet., 47, 156-162.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.49 coexpression of and functional complementation by His-tagged soluble Thi20p or Thi21p isozymes with a thiamin synthetase in an Escherichia coli strain SN372L-3-9-10 deficient in thiamin biosynthesis Saccharomyces cerevisiae
2.7.4.7 expression in Escherichia coli Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.49 additional information phosphomethylpyrimidine kinase activity, EC 2.7.4.7, is abolished in C-terminally truncated isozymes Thi20p and Thi21p, the mutant yeast strains sow no thiamin phosphate synthesis activity Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.49 Mg2+ at 20 mM MgCl2 Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.49 65000
-
 x * 65000, recombinant His-tagged isozymes Thi20p or Thi21p, SDS-PAGE Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.49 ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine Saccharomyces cerevisiae
-
 ADP + 4-amino-5-phosphomethyl-2-methylpyrimidine
-
 ?
2.7.1.49 ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine Saccharomyces cerevisiae YPH500
-
 ADP + 4-amino-5-phosphomethyl-2-methylpyrimidine
-
 ?
2.7.1.49 ATP + 4-amino-5-phosphomethyl-2-methylpyrimidine Saccharomyces cerevisiae enzyme also performs the reaction of EC 2.7.4.7, phosphomethylpyrimidine kinase ADP + 4-amino-5-diphosphomethyl-2-methylpyrimidine
-
 ?
2.7.1.49 ATP + 4-amino-5-phosphomethyl-2-methylpyrimidine Saccharomyces cerevisiae YPH500 enzyme also performs the reaction of EC 2.7.4.7, phosphomethylpyrimidine kinase ADP + 4-amino-5-diphosphomethyl-2-methylpyrimidine
-
 ?
2.7.4.7 ATP + 4-amino-2-methyl-phosphomethylpyrimidine Saccharomyces cerevisiae enzyme is required for thiamin biosynthesis ADP + 4-amino-2-methyl-5-diphosphomethylpyrimidine
-
 ?
2.7.4.7 ATP + 4-amino-2-methyl-phosphomethylpyrimidine Saccharomyces cerevisiae YPH500 enzyme is required for thiamin biosynthesis ADP + 4-amino-2-methyl-5-diphosphomethylpyrimidine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.49 Saccharomyces cerevisiae
-
 2 isozymes Thi20p and Thi21p encoded by 2 redundant genes THI20 and THI21
-
2.7.1.49 Saccharomyces cerevisiae YPH500
-
 2 isozymes Thi20p and Thi21p encoded by 2 redundant genes THI20 and THI21
-
2.7.4.7 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.49 recombinant His-tagged isozymes Thi20p or Thi21p from Escherichia coli Saccharomyces cerevisiae
2.7.4.7 partial Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.49 additional information
-
 thiamine synthesis from hydroxymethylpyrimidine activities in wild-type and mutant strains Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.49 ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine
-
 Saccharomyces cerevisiae ADP + 4-amino-5-phosphomethyl-2-methylpyrimidine
-
 ?
2.7.1.49 ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine
-
 Saccharomyces cerevisiae YPH500 ADP + 4-amino-5-phosphomethyl-2-methylpyrimidine
-
 ?
2.7.1.49 ATP + 4-amino-5-phosphomethyl-2-methylpyrimidine enzyme also performs the reaction of EC 2.7.4.7, phosphomethylpyrimidine kinase Saccharomyces cerevisiae ADP + 4-amino-5-diphosphomethyl-2-methylpyrimidine
-
 ?
2.7.1.49 ATP + 4-amino-5-phosphomethyl-2-methylpyrimidine enzyme also performs the reaction of EC 2.7.4.7, phosphomethylpyrimidine kinase Saccharomyces cerevisiae YPH500 ADP + 4-amino-5-diphosphomethyl-2-methylpyrimidine
-
 ?
2.7.1.49 additional information the isozymes Thi20p and Thi21p are bifunctional and show both phosphomethylpyrimidine kinase, EC 2.7.4.7, and hydroxymethylpyrimidine kinase, EC 2.7.1.49, activity, Thi20p exhibits higher activity than Thi21p Saccharomyces cerevisiae ?
-
 ?
2.7.1.49 additional information the isozymes Thi20p and Thi21p are bifunctional and show both phosphomethylpyrimidine kinase, EC 2.7.4.7, and hydroxymethylpyrimidine kinase, EC 2.7.1.49, activity, Thi20p exhibits higher activity than Thi21p Saccharomyces cerevisiae YPH500 ?
-
 ?
2.7.4.7 ATP + 4-amino-2-methyl-phosphomethylpyrimidine enzyme is required for thiamin biosynthesis Saccharomyces cerevisiae ADP + 4-amino-2-methyl-5-diphosphomethylpyrimidine
-
 ?
2.7.4.7 ATP + 4-amino-2-methyl-phosphomethylpyrimidine the Thi20p protein has a greater ability than the Thi21p protein to catalyze the reaction Saccharomyces cerevisiae ADP + 4-amino-2-methyl-5-diphosphomethylpyrimidine
-
 ?
2.7.4.7 ATP + 4-amino-2-methyl-phosphomethylpyrimidine enzyme is required for thiamin biosynthesis Saccharomyces cerevisiae YPH500 ADP + 4-amino-2-methyl-5-diphosphomethylpyrimidine
-
 ?
2.7.4.7 ATP + 4-amino-2-methyl-phosphomethylpyrimidine the Thi20p protein has a greater ability than the Thi21p protein to catalyze the reaction Saccharomyces cerevisiae YPH500 ADP + 4-amino-2-methyl-5-diphosphomethylpyrimidine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.49 ? x * 65000, recombinant His-tagged isozymes Thi20p or Thi21p, SDS-PAGE Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
2.7.1.49 4-amino-5-hydroxymethyl-2-methylpyrimidine kinase
-
 Saccharomyces cerevisiae
2.7.1.49 HMP kinase
-
 Saccharomyces cerevisiae
2.7.1.49 More the enzyme is probably identical with EC 2.7.4.7, 4-amino-5-hydroxymethyl-2-methylpyrimidine monophosphate kinase, or HMP-P kinase Saccharomyces cerevisiae
2.7.4.7 2-methyl-4-amino-5-hydroxymethylpyrimidine monophosphate kinase
-
 Saccharomyces cerevisiae
2.7.4.7 HMP-P kinase
-
 Saccharomyces cerevisiae
2.7.4.7 Thi20p protein
-
 Saccharomyces cerevisiae
2.7.4.7 Thi21p protein
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.49 37
-
 assay at Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.49 7.5
-
 assay at Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.49 ATP
-
 Saccharomyces cerevisiae