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Literature summary extracted from

  • Papadopoulos, A.I.; Polemitou, I.; Laifi, P.; Yiangou, A.; Tananaki, C.
    Glutathione S-transferase in the insect Apis mellifera macedonica kinetic characteristics and effect of stress on the expression of GST isoenzymes in the adult worker bee (2004), Comp. Biochem. Physiol. C, 139, 93-97.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.18 bromosulfophthalein 0.053 mM, 50% inhibition, isoenzyme I; 1.24 mM, 50% inhibition, isoenzyme II Apis mellifera macedonica
2.5.1.18 chlorotriphenyltin 0.33 mM, 50% inhibition, isoenzyme I; 6.4 mM, 50% inhibition, isoenzyme II Apis mellifera macedonica
2.5.1.18 Cibacron blue 0.053 mM, 50% inhibition, isoenzyme I; 0.073 mM, 50% inhibition, isoenzyme II Apis mellifera macedonica
2.5.1.18 Hematin 0.13 mM, 50% inhibition, isoenzyme I; 1.81 mM, 50% inhibition, isoenzyme II Apis mellifera macedonica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.18 0.00026
-
 1-chloro-2,4-dinitrobenzene isoenzyme I Apis mellifera macedonica
2.5.1.18 0.00027
-
 GSH isoenzyme I Apis mellifera macedonica
2.5.1.18 0.00089
-
 1-chloro-2,4-dinitrobenzene isoenzyme I Apis mellifera macedonica
2.5.1.18 0.0012
-
 GSH isoenzyme I Apis mellifera macedonica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.18 25000
-
 1 * 29000 + 1 * 25000, SDS-PAGE Apis mellifera macedonica
2.5.1.18 29000
-
 1 * 29000 + 1 * 25000, SDS-PAGE Apis mellifera macedonica

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.18 Apis mellifera macedonica
-
 isoenzyme I
-
2.5.1.18 Apis mellifera macedonica
-
 isoenzyme II
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.18
-
 Apis mellifera macedonica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.18 whole body homogenate of adult worker bee Apis mellifera macedonica
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.18 1-chloro-2,4-dinitrobenzene + GSH
-
 Apis mellifera macedonica ?
-
 ?
2.5.1.18 1-chloro-2,4-dinitrobenzene + GSH
-
 Apis mellifera macedonica S-(2,4-dinitrophenyl)glutathione + HCl
-
 ?
2.5.1.18 bromosulfophthalein + GSH 10.3% of the activity with 1-chloro-2,4-dinitrobenzene, isoenzyme II Apis mellifera macedonica ?
-
 ?
2.5.1.18 bromosulfophthalein + GSH 8.6% of the activity with 1-chloro-2,4-dinitrobenzene, isoenzyme I Apis mellifera macedonica ?
-
 ?
2.5.1.18 cumene hydroperoxide + GSH 106% of the activity with 1-chloro-2,4-dinitrobenzene, isoenzyme I Apis mellifera macedonica ?
-
 ?
2.5.1.18 cumene hydroperoxide + GSH 11.9% of the activity with 1-chloro-2,4-dinitrobenzene, isoenzyme II Apis mellifera macedonica ?
-
 ?
2.5.1.18 ethacrynic acid + GSH 15% of the activity with 1-chloro-2,4-dinitrobenzene, isoenzyme I Apis mellifera macedonica ?
-
 ?
2.5.1.18 GSH reductase + GSH 271% of the activity with 1-chloro-2,4-dinitrobenzene, isoenzyme II Apis mellifera macedonica ?
-
 ?
2.5.1.18 GSH reductase + GSH 87% of the activity with 1-chloro-2,4-dinitrobenzene, isoenzyme I Apis mellifera macedonica ?
-
 ?
2.5.1.18 trans-phenyl-butenone + GSH 312% of the activity with 1-chloro-2,4-dinitrobenzene, isoenzyme II Apis mellifera macedonica ?
-
 ?
2.5.1.18 trans-phenyl-butenone + GSH 71% of the activity with 1-chloro-2,4-dinitrobenzene, isoenzyme I Apis mellifera macedonica ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.18 dimer 1 * 29000 + 1 * 25000, SDS-PAGE Apis mellifera macedonica