Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yamamoto, K.; Zhang, P.; Miake, F.; Kashige, N.; Aso, Y.; Banno, Y.; Fujii, H.
    Cloning, expression and characterization of theta-class glutathione S-transferase from the silkworm, Bombyx mori (2005), Comp. Biochem. Physiol. B, 141, 340-346.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.18 overexpression in Escherichia coli Bombyx mori

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.18 0.14
-
 4-hydroxynonenal
-
 Bombyx mori
2.5.1.18 0.48
-
 1-chloro-2,4-dinitrobenzene
-
 Bombyx mori
2.5.1.18 2.43
-
 Ethacrynic acid
-
 Bombyx mori
2.5.1.18 4.35
-
 4-nitrophenyl acetate
-
 Bombyx mori

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.18 24000
-
 x * 24000, SDS-PAGE Bombyx mori

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.18 Bombyx mori
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.18
-
 Bombyx mori

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.18 25.6
-
-
 Bombyx mori

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.18 1-chloro-2,4-dinitrobenzene + GSH
-
 Bombyx mori S-(2,4-dinitrophenyl)glutathione + HCl
-
 ?
2.5.1.18 4-hydroxynonenal + GSH 90% of the activity with 1-chloro-2,4-dinitrobenzene Bombyx mori ?
-
 ?
2.5.1.18 4-nitrophenyl acetate + GSH 198% of the activity with 1-chloro-2,4-dinitrobenzene Bombyx mori ?
-
 ?
2.5.1.18 ethacrynic acid + GSH 24% of the activity with 1-chloro-2,4-dinitrobenzene Bombyx mori ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.18 ? x * 24000, SDS-PAGE Bombyx mori

Synonyms

EC Number Synonyms Comment Organism
2.5.1.18 GSTT1
-
 Bombyx mori

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.18 50
-
 30 min, stable below Bombyx mori

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.18 5 9
-
 Bombyx mori

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.18 4 10 pH 4: about 75% of maximal activity, pH 10: about 60% of maximal activity Bombyx mori

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.5.1.18 1 2 4°C, 24 h, about 30% loss of activity Bombyx mori
2.5.1.18 4
-
 4°C, 24 h, about 50% loss of activity Bombyx mori
2.5.1.18 5 10 4°C, 24 h, about 20% loss of activity Bombyx mori