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Literature summary extracted from
- Access of the substrate to the active site of yeast oxidosqualene cyclase: an inhibition and site-directed mutagenesis approach (2005), ChemBioChem, 6, 2221-2228.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.4.99.7 | - |
Saccharomyces cerevisiae |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 5.4.99.7 | - |
Saccharomyces cerevisiae |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 5.4.99.7 | C457D | less active than the wild-type | Saccharomyces cerevisiae |
| 5.4.99.7 | C457D/A525C | similar activity as the wild-type | Saccharomyces cerevisiae |
| 5.4.99.7 | C457D/E526A | decrease in enzyme functionality | Saccharomyces cerevisiae |
| 5.4.99.7 | C457D/E526C | very sensitive to the thiol-reacting agent dodecylmaleimide, specific activity and thermal stability are severely reduced | Saccharomyces cerevisiae |
| 5.4.99.7 | C457D/E526D | no effect on catalytic avtivity | Saccharomyces cerevisiae |
| 5.4.99.7 | C457D/E526Q | no effect on catalytic avtivity | Saccharomyces cerevisiae |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 5.4.99.7 | mutants C457D/E526C and C457D/E526A are not stable at 35°C | Saccharomyces cerevisiae |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.99.7 | dodecyl-maleimide | mutant C457D shows 41.6% inhibition at 1 mM, 10.0% at 0.2 mM, and 7.2% at 0.025 mM, mutant C457D/E526A shows 9.0% inhibition at 0.2 mM and 0.0% at 0.025 mM, mutant C457D/A525C shows 86.0% inhibition at 1 mM, 30.5% at 0.2 mM, and 1.0% at 0.025 mM, mutant C457D/E526C shows 89.6% inhibition at 0.2 mM, 74.7% at 0.025 mM, and 45.1% at 0.01 mM | Saccharomyces cerevisiae |  |
| 5.4.99.7 | N,N-Dimethylformamide | - |
Saccharomyces cerevisiae | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.7 | (3S)-2,3-oxidosqualene | Saccharomyces cerevisiae | - |
lanosterol | - |
? | |
| 5.4.99.7 | (3S)-2,3-oxidosqualene | Saccharomyces cerevisiae SMY8 | - |
lanosterol | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.99.7 | Saccharomyces cerevisiae | - |
- |
- |
| 5.4.99.7 | Saccharomyces cerevisiae SMY8 | - |
- |
- |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.7 | 0.0000015 | - |
mutant C457/E526A at 25°C | Saccharomyces cerevisiae |
| 5.4.99.7 | 0.0000015 | - |
mutant C457/E526C at 25°C | Saccharomyces cerevisiae |
| 5.4.99.7 | 0.0000025 | - |
mutant C457/E526C at 35°C | Saccharomyces cerevisiae |
| 5.4.99.7 | 0.0000048 | - |
mutant C457/E526A at 35°C | Saccharomyces cerevisiae |
| 5.4.99.7 | 0.0000063 | - |
mutant C457 at 25°C | Saccharomyces cerevisiae |
| 5.4.99.7 | 0.0000087 | - |
mutant C457/E526D at 25°C | Saccharomyces cerevisiae |
| 5.4.99.7 | 0.0000097 | - |
mutant C457/E526Q at 25°C | Saccharomyces cerevisiae |
| 5.4.99.7 | 0.0000113 | - |
mutant C457/E525C at 25°C | Saccharomyces cerevisiae |
| 5.4.99.7 | 0.0000128 | - |
mutant C457/E526D at 35°C | Saccharomyces cerevisiae |
| 5.4.99.7 | 0.0000172 | - |
wild-type, at 25°C | Saccharomyces cerevisiae |
| 5.4.99.7 | 0.0000192 | - |
mutant C457/E526Q at 35°C | Saccharomyces cerevisiae |
| 5.4.99.7 | 0.000021 | - |
mutant C457/E525C at 35°C | Saccharomyces cerevisiae |
| 5.4.99.7 | 0.0000213 | - |
mutant C457 at 35°C | Saccharomyces cerevisiae |
| 5.4.99.7 | 0.0000292 | - |
wild-type, at 35°C | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.7 | (3S)-2,3-oxidosqualene | - |
Saccharomyces cerevisiae | lanosterol | - |
? | |
| 5.4.99.7 | (3S)-2,3-oxidosqualene | - |
Saccharomyces cerevisiae SMY8 | lanosterol | - |
? | |
| 5.4.99.7 | squalene | - |
Saccharomyces cerevisiae | ? | - |
? | |
| 5.4.99.7 | squalene | - |
Saccharomyces cerevisiae SMY8 | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.4.99.7 | lanosterol synthase | - |
Saccharomyces cerevisiae |
| 5.4.99.7 | OSC | - |
Saccharomyces cerevisiae |
| 5.4.99.7 | Oxidosqualene cyclase | - |
Saccharomyces cerevisiae |
| 5.4.99.7 | SceOSC | - |
Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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