Literature summary extracted from

Habermeyer, M.; Fritz, J.; Barthelmes, H.U.; Christensen, M.O.; Larsen, M.K.; Boege, F.; Marko, D.
Anthocyanidins modulate the activity of human DNA topoisomerases I and II and affect cellular DNA integrity (2005), Chem. Res. Toxicol., 18, 1395-1404.

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
5.6.2.2	2-(4-hydroxy-3-methoxyphenyl)chromenylium-3,5,7-triol	anthocyanidin, inhibition of isozyme IIbeta decatenation activity	Homo sapiens
5.6.2.2	anthocyanidins	modulate the enzyme activity and affect cellular DNA integrity, high enzyme inhibition by anthocyanidins with vicinal hydroxyl groups like delphinidin or cyanidin, competitive to ethidium bromide in DNA intercalation	Homo sapiens
5.6.2.2	cyanidin	anthocyanidin, complete inhibition of enzyme, isozyme IIalpha and IIbeta, decatenation activity at 0.01 mM, no stabilization of the DNA-enzyme intermediate in contrast to topoisomerase poisons	Homo sapiens
5.6.2.2	delphinidin	anthocyanidin, complete inhibition of enzyme, isozyme IIalpha and IIbeta, decatenation activity at 0.01 mM, no stabilization of the DNA-enzyme intermediate in contrast to topoisomerase poisons	Homo sapiens
5.6.2.2	malvidin	anthocyanidin, inhibition of isozyme IIbeta decatenation activity	Homo sapiens
5.6.2.2	pelargonidin	anthocyanidin, inhibition of isozyme IIbeta decatenation activity	Homo sapiens

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
5.6.2.2	Mg2+	-	Homo sapiens

Organism

EC Number	Organism	UniProt	Comment	Textmining
5.6.2.2	Homo sapiens	-	two isozymes IIalpha and IIbeta	-

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
5.6.2.2	HT-29 cell	colon adenocarcinoma cell line	Homo sapiens	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5.6.2.2	DNA + ATP + H2O	decatenation of DNA	Homo sapiens	DNA + ADP + phosphate	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
5.6.2.2	Topoisomerase II	-	Homo sapiens

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
5.6.2.2	37	-	assay at	Homo sapiens

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5.6.2.2	7.9	-	assay at	Homo sapiens

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
5.6.2.2	ATP	-	Homo sapiens

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)