BRENDA - Enzyme Database show

Structure and function of cellular deoxyribonucleoside kinases

Eriksson, S.; Munch-Petersen, B.; Johansson, K.; Eklund, H.; Cell. Mol. Life Sci. 59, 1327-1346 (2002)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.113
Triton X-100
-
Homo sapiens
Application
EC Number
Application
Commentary
Organism
2.7.1.145
medicine
enzyme with increased specificity towards certain analogues can be used as a suicide gene in combined gene/chemotherapy treatment of cancer
Bombyx mori
2.7.1.145
medicine
enzyme with increased specificity towards certain analogues can be used as a suicide gene in combined gene/chemotherapy treatment of cancer
Drosophila melanogaster
2.7.1.145
synthesis
enzyme can be utilized in industrial large-scale production of dNTPs and analogues, used forDNA synthetic reactions, giving a higher yield and less toxic byproducts compared to chemical processes
Bombyx mori
2.7.1.145
synthesis
enzyme can be utilized in industrial large-scale production of dNTPs and analogues, used for DNA synthetic reactions, giving a higher yield and less toxic byproducts compared to chemical processes
Drosophila melanogaster
Cloned(Commentary)
EC Number
Commentary
Organism
2.7.1.74
the single copy gene is located on chromosome 4q13.3-q21.1
Homo sapiens
2.7.1.113
gene is localized on chromosome 2q13
Homo sapiens
2.7.1.145
DNA and amino acid sequence determination and analysis, gene is located on chromosome 3 at position 91D-91E, expression of wild-type and mutant enzymes in Escherichia coli TK-deficient strain KY895
Drosophila melanogaster
Crystallization (Commentary)
EC Number
Crystallization
Organism
2.7.1.113
enzyme in complex with ATP
Homo sapiens
Engineering
EC Number
Amino acid exchange
Commentary
Organism
2.7.1.145
N45D/N64D
site-directed mutagenesis, mutant shows increased drug sensitivity and decreased inhibition by dTTP compared to the wild-type enzyme
Drosophila melanogaster
General Stability
EC Number
General Stability
Organism
2.7.1.113
Triton X-100 and ATP stabilize
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.74
dCTP
feedback inhibition, competitive versus phosphate donors
Homo sapiens
2.7.1.113
dATP
feedback inhibition
Homo sapiens
2.7.1.113
dGTP
feedback inhibition
Homo sapiens
2.7.1.145
dTTP
competitive versus ATP, feedback inhibition
Bombyx mori
2.7.1.145
dTTP
competitive versus ATP, feedback inhibition
Drosophila melanogaster
2.7.1.145
additional information
no inhibition by dNTPs other than dTTP
Bombyx mori
2.7.1.145
additional information
no inhibition by dNTPs other than dTTP
Drosophila melanogaster
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.74
additional information
-
additional information
no Michaelis-Menten kinetics, but negative cooperativity
Homo sapiens
2.7.1.145
additional information
-
additional information
positive cooperativity with all 4 natural deoxyribonucleosides, no hyperbolic Michaelis-Menten substrate kinetics
Bombyx mori
2.7.1.145
additional information
-
additional information
straight forward hyperbolic kinetic reaction mechanism following a compulsory ordered steady-state reaction mechanism with formation of a ternary complex
Drosophila melanogaster
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.74
cytoplasm
-
Homo sapiens
5737
-
2.7.1.74
nucleus
enzyme possesses a nuclear localization signal at the N-terminus
Homo sapiens
5634
-
2.7.1.113
mitochondrial matrix
-
Homo sapiens
5759
-
2.7.1.113
mitochondrion
enzyme possesses an N-terminal mitochondrial import signal
Homo sapiens
5739
-
2.7.1.113
additional information
the enzyme leaks out into the cytosol during cell apoptosis
Homo sapiens
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.74
Mg2+
-
Homo sapiens
2.7.1.113
Mg2+
-
Homo sapiens
2.7.1.145
Mg2+
dependent on
Bombyx mori
2.7.1.145
Mg2+
dependent on
Drosophila melanogaster
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.113
30000
-
2 * 30000
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.74
ATP + 2'-deoxycytidine
Homo sapiens
-
ADP + 2'-deoxycytidine 5'-phosphate
-
-
?
2.7.1.74
UTP + 2'-deoxycytidine
Homo sapiens
-
UDP + 2'-deoxycytidine 5'-phosphate
-
-
?
2.7.1.113
ATP + 2'-deoxyadenosine
Homo sapiens
-
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
?
2.7.1.113
ATP + 2'-deoxyguanosine
Homo sapiens
-
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
?
2.7.1.113
UTP + 2'-deoxyadenosine
Homo sapiens
-
UDP + 2'-deoxyadenosine 5'-phosphate
-
-
?
2.7.1.113
UTP + 2'-deoxyguanosine
Homo sapiens
-
UDP + 2'-deoxyguanosine 5'-phosphate
-
-
?
2.7.1.145
ATP + 2'-deoxyadenosine
Drosophila melanogaster
-
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
?
2.7.1.145
ATP + 2'-deoxycytidine
Drosophila melanogaster
-
ADP + 2'-deoxycytidine 5'-phosphate
-
-
?
2.7.1.145
ATP + 2'-deoxyguanosine
Drosophila melanogaster
-
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
?
2.7.1.145
ATP + 2'-deoxythymidine
Drosophila melanogaster
-
ADP + 2'-deoxythymidine 5'-phosphate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.74
Homo sapiens
P27707
-
-
2.7.1.113
Homo sapiens
Q16854
constitutive expression
-
2.7.1.145
Bombyx mori
-
-
-
2.7.1.145
Drosophila melanogaster
Q9XZT6
dNK is the only deoxyriconucleoside kinase in the fruitfly
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
2.7.1.74
additional information
posttranscriptional regulation of the enzyme
Homo sapiens
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.113
enzyme activity is stabilized during purification in presence of Triton X-100 and ATP
Homo sapiens
Reaction
EC Number
Reaction
Commentary
Organism
2.7.1.74
NTP + deoxycytidine = NDP + dCMP
substrate-enzyme interactions, bi bi random reaction mechanism with ATP, but ordered with UTP binding before the acceptor substrate, substrate binding site structures
Homo sapiens
2.7.1.113
ATP + deoxyguanosine = ADP + dGMP
substrate interactions, reaction mechanism
Homo sapiens
2.7.1.145
ATP + a 2'-deoxyribonucleoside = ADP + a 2'-deoxyribonucleoside 5'-phosphate
positive cooperativity mechanism, substrate and nucleotide interactions and binding sites, overview
Bombyx mori
2.7.1.145
ATP + a 2'-deoxyribonucleoside = ADP + a 2'-deoxyribonucleoside 5'-phosphate
straight forward hyperbolic kinetic reaction mechanism following a compulsory ordered steady-state reaction mechanism with formation of a ternary complex, substrate interactions
Drosophila melanogaster
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.74
colonic mucosa
-
Homo sapiens
-
2.7.1.74
kidney
very low enzyme expression
Homo sapiens
-
2.7.1.74
liver
very low enzyme expression
Homo sapiens
-
2.7.1.74
lymphoid tissue
high enzyme expression
Homo sapiens
-
2.7.1.74
additional information
tissue specific enzyme expression
Homo sapiens
-
2.7.1.74
muscle
very low enzyme expression
Homo sapiens
-
2.7.1.74
pancreas
very low enzyme expression
Homo sapiens
-
2.7.1.113
brain
-
Homo sapiens
-
2.7.1.113
liver
-
Homo sapiens
-
2.7.1.113
lymphoid tissue
-
Homo sapiens
-
2.7.1.113
skin
-
Homo sapiens
-
2.7.1.113
spleen
-
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.74
additional information
-
substrate specificity
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
ATP + 2',3'-dideoxycytidine
i.e. zalcitabine
661453
Homo sapiens
ADP + 2',3'-dideoxycytidine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + 2'-deoxyadenosine
-
661453
Homo sapiens
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + 2'-deoxycytidine
-
661453
Homo sapiens
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + 2'-deoxyguanosine
-
661453
Homo sapiens
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + 2-chloro-2'-deoxyadenosine
i.e. cladribine
661453
Homo sapiens
ADP + 2-chloro-2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + arabinosyl adenine
-
661453
Homo sapiens
ADP + arabinosyl adenine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + arabinosyl cytosine
i.e. cytosar
661453
Homo sapiens
ADP + arabinosyl cytosine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + beta-L-2',3'-dideoxy-3'-thiacytidine
-
661453
Homo sapiens
ADP + beta-L-2',3'-dideoxy-3'-thiacytidine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + difluoro-arabinofuranosyl adenine
i.e. fludarabine
661453
Homo sapiens
ADP + difluoro-arabinofuranosyl adenine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + difluorodeoxycytidine
i.e. gemcitabine
661453
Homo sapiens
ADP + difluorodeoxycytidine 5'-phosphate
-
-
-
?
2.7.1.74
additional information
substrate-enzyme interactions, overview, enzyme prefers nucleosides with sugars in the S-conformation, i.e C2'-endo-C3'-exo, binding of substrate induces conformational changes, the phosphate donor influences the acceptor substrate specificity, mechanism, overview
661453
Homo sapiens
?
-
-
-
-
2.7.1.74
UTP + 2',3'-dideoxycytidine
i.e. zalcitabine
661453
Homo sapiens
UDP + 2',3'-dideoxycytidine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + 2'-deoxyadenosine
-
661453
Homo sapiens
UDP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + 2'-deoxycytidine
-
661453
Homo sapiens
UDP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + 2'-deoxyguanosine
-
661453
Homo sapiens
UDP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + 2-chloro-2'-deoxyadenosine
i.e. cladribine
661453
Homo sapiens
UDP + 2-chloro-2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + arabinosyl adenine
-
661453
Homo sapiens
UDP + arabinosyl adenine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + arabinosyl cytosine
i.e. cytosar
661453
Homo sapiens
UDP + arabinosyl cytosine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + beta-L-2',3'-dideoxy-3'-thiacytidine
-
661453
Homo sapiens
UDP + beta-L-2',3'-dideoxy-3'-thiacytidine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + beta-L-dioxolane-cytidine
antitumor drug
661453
Homo sapiens
UDP + beta-L-dioxolane-cytidine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + difluoro-arabinofuranosyl adenine
i.e. fludarabine
661453
Homo sapiens
UDP + difluoro-arabinofuranosyl adenine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + difluorodeoxycytidine
i.e. gemcitabine
661453
Homo sapiens
UDP + difluorodeoxycytidine 5'-phosphate
-
-
-
?
2.7.1.113
ATP + 1-beta-D-arabinofuranosyladenine
-
661453
Homo sapiens
ADP + 1-beta-D-arabinofuranosyladenine 5'-phosphate
-
-
-
?
2.7.1.113
ATP + 2',2'-difluorodeoxyguanosine
-
661453
Homo sapiens
ADP + 2',2'-difluorodeoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.113
ATP + 2'-deoxyadenosine
-
661453
Homo sapiens
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.113
ATP + 2'-deoxyguanosine
-
661453
Homo sapiens
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.113
ATP + 2'-deoxyinosine
-
661453
Homo sapiens
ADP + 2'-deoxyinosine 5'-phosphate
-
-
-
?
2.7.1.113
ATP + 2-chloro-2'-arabinofluoro-2'-deoxyadenosine
-
661453
Homo sapiens
ADP + 2-chloro-2'-arabinofluoro-2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.113
ATP + 2-chlorodeoxyadenosine
-
661453
Homo sapiens
ADP + 2-chlorodeoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.113
ATP + 9-beta-D-arabinofuranosylguanine
-
661453
Homo sapiens
ADP + 9-beta-D-arabinofuranosylguanine 5'-phosphate
-
-
-
?
2.7.1.113
ATP + ganciclovir
i.e. 9-(1,3-dihydroxy-2-propoxymethyl)-guanine, low activity
661453
Homo sapiens
ADP + ganciclovir phosphate
-
-
-
?
2.7.1.113
ATP + penciclovir
i.e. 9-(2-hydroxy-1-hydroxymethyl-ethoxymethyl)-guanine, low activity
661453
Homo sapiens
ADP + penciclovir 5'-phosphate
-
-
-
?
2.7.1.113
additional information
substrate interactions, overview, enzyme shows relaxed enantioselectivity, accepting D- and L-enantiomers of beta-deoxyguanosine but with lower activity than alpha-L-deoxyguanosine, and beta-L-deoxyadenosine, enzyme accepts purine deoxynucleosides and analogues as substrates utilizing a triphosphate donor substrate
661453
Homo sapiens
?
-
-
-
-
2.7.1.113
UTP + 2'-deoxyadenosine
-
661453
Homo sapiens
UDP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.113
UTP + 2'-deoxyguanosine
-
661453
Homo sapiens
UDP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.113
UTP + 2'-deoxyinosine
-
661453
Homo sapiens
UDP + 2'-deoxyinosine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 1-beta-D-arabinofuranosylcytosine
-
661453
Drosophila melanogaster
ADP + 1-beta-D-arabinofuranosylcytosine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 1-beta-D-arabinofuranosylthymine
-
661453
Drosophila melanogaster
ADP + 1-beta-D-arabinofuranosylthymine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxyadenosine
-
661453
Drosophila melanogaster
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxycytidine
-
661453
Drosophila melanogaster
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxyguanosine
-
661453
Drosophila melanogaster
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxythymidine
-
661453
Drosophila melanogaster
ADP + 2'-deoxythymidine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2-chloro-2'-deoxyadenosine
-
661453
Drosophila melanogaster
ADP + 2-chloro-2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 5-fluoro-2'-deoxyuridine
-
661453
Drosophila melanogaster
ADP + 5-fluoro-2'-deoxyuridine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + E-5-(2-bromovinyl)-2'-deoxyuridine
-
661453
Drosophila melanogaster
ADP + E-5-(2-bromovinyl)-2'-deoxyuridine phosphate
-
-
-
?
2.7.1.145
additional information
broad substrate specificity
661453
Drosophila melanogaster
?
-
-
-
-
2.7.1.145
additional information
substrate specificity, no activity with purine ribonucleosides and dideoxynucleosides
661453
Bombyx mori
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
2.7.1.74
More
3D-structure analysis, dCK structure model, overview
Homo sapiens
2.7.1.113
dimer
2 * 30000
Homo sapiens
2.7.1.113
More
ternary and quarternary structure analysis, overview
Homo sapiens
2.7.1.145
dimer
-
Bombyx mori
2.7.1.145
dimer
-
Drosophila melanogaster
2.7.1.145
More
ternary and quarternary structure analysis, overview
Bombyx mori
2.7.1.145
More
ternary and quarternary structure analysis, overview
Drosophila melanogaster
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.74
ATP
-
Homo sapiens
2.7.1.74
additional information
enzyme lalso utilizes other nucleoside triphosphates as phosphate donors
Homo sapiens
2.7.1.74
UTP
-
Homo sapiens
2.7.1.113
ATP
binding site structure and interactions, Gln111, Arg118, Met99, and Asp147 are involved, overview
Homo sapiens
2.7.1.113
additional information
enzyme accepts several triphosphate donor substrates with preference for ATP and UTP
Homo sapiens
2.7.1.113
UTP
-
Homo sapiens
2.7.1.145
ATP
-
Drosophila melanogaster
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.113
Triton X-100
-
Homo sapiens
Application (protein specific)
EC Number
Application
Commentary
Organism
2.7.1.145
medicine
enzyme with increased specificity towards certain analogues can be used as a suicide gene in combined gene/chemotherapy treatment of cancer
Bombyx mori
2.7.1.145
medicine
enzyme with increased specificity towards certain analogues can be used as a suicide gene in combined gene/chemotherapy treatment of cancer
Drosophila melanogaster
2.7.1.145
synthesis
enzyme can be utilized in industrial large-scale production of dNTPs and analogues, used forDNA synthetic reactions, giving a higher yield and less toxic byproducts compared to chemical processes
Bombyx mori
2.7.1.145
synthesis
enzyme can be utilized in industrial large-scale production of dNTPs and analogues, used for DNA synthetic reactions, giving a higher yield and less toxic byproducts compared to chemical processes
Drosophila melanogaster
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.74
the single copy gene is located on chromosome 4q13.3-q21.1
Homo sapiens
2.7.1.113
gene is localized on chromosome 2q13
Homo sapiens
2.7.1.145
DNA and amino acid sequence determination and analysis, gene is located on chromosome 3 at position 91D-91E, expression of wild-type and mutant enzymes in Escherichia coli TK-deficient strain KY895
Drosophila melanogaster
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.74
ATP
-
Homo sapiens
2.7.1.74
additional information
enzyme lalso utilizes other nucleoside triphosphates as phosphate donors
Homo sapiens
2.7.1.74
UTP
-
Homo sapiens
2.7.1.113
ATP
binding site structure and interactions, Gln111, Arg118, Met99, and Asp147 are involved, overview
Homo sapiens
2.7.1.113
additional information
enzyme accepts several triphosphate donor substrates with preference for ATP and UTP
Homo sapiens
2.7.1.113
UTP
-
Homo sapiens
2.7.1.145
ATP
-
Drosophila melanogaster
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
2.7.1.113
enzyme in complex with ATP
Homo sapiens
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
2.7.1.145
N45D/N64D
site-directed mutagenesis, mutant shows increased drug sensitivity and decreased inhibition by dTTP compared to the wild-type enzyme
Drosophila melanogaster
General Stability (protein specific)
EC Number
General Stability
Organism
2.7.1.113
Triton X-100 and ATP stabilize
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.74
dCTP
feedback inhibition, competitive versus phosphate donors
Homo sapiens
2.7.1.113
dATP
feedback inhibition
Homo sapiens
2.7.1.113
dGTP
feedback inhibition
Homo sapiens
2.7.1.145
dTTP
competitive versus ATP, feedback inhibition
Bombyx mori
2.7.1.145
dTTP
competitive versus ATP, feedback inhibition
Drosophila melanogaster
2.7.1.145
additional information
no inhibition by dNTPs other than dTTP
Bombyx mori
2.7.1.145
additional information
no inhibition by dNTPs other than dTTP
Drosophila melanogaster
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.74
additional information
-
additional information
no Michaelis-Menten kinetics, but negative cooperativity
Homo sapiens
2.7.1.145
additional information
-
additional information
positive cooperativity with all 4 natural deoxyribonucleosides, no hyperbolic Michaelis-Menten substrate kinetics
Bombyx mori
2.7.1.145
additional information
-
additional information
straight forward hyperbolic kinetic reaction mechanism following a compulsory ordered steady-state reaction mechanism with formation of a ternary complex
Drosophila melanogaster
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.74
cytoplasm
-
Homo sapiens
5737
-
2.7.1.74
nucleus
enzyme possesses a nuclear localization signal at the N-terminus
Homo sapiens
5634
-
2.7.1.113
mitochondrial matrix
-
Homo sapiens
5759
-
2.7.1.113
mitochondrion
enzyme possesses an N-terminal mitochondrial import signal
Homo sapiens
5739
-
2.7.1.113
additional information
the enzyme leaks out into the cytosol during cell apoptosis
Homo sapiens
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.74
Mg2+
-
Homo sapiens
2.7.1.113
Mg2+
-
Homo sapiens
2.7.1.145
Mg2+
dependent on
Bombyx mori
2.7.1.145
Mg2+
dependent on
Drosophila melanogaster
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.113
30000
-
2 * 30000
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.74
ATP + 2'-deoxycytidine
Homo sapiens
-
ADP + 2'-deoxycytidine 5'-phosphate
-
-
?
2.7.1.74
UTP + 2'-deoxycytidine
Homo sapiens
-
UDP + 2'-deoxycytidine 5'-phosphate
-
-
?
2.7.1.113
ATP + 2'-deoxyadenosine
Homo sapiens
-
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
?
2.7.1.113
ATP + 2'-deoxyguanosine
Homo sapiens
-
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
?
2.7.1.113
UTP + 2'-deoxyadenosine
Homo sapiens
-
UDP + 2'-deoxyadenosine 5'-phosphate
-
-
?
2.7.1.113
UTP + 2'-deoxyguanosine
Homo sapiens
-
UDP + 2'-deoxyguanosine 5'-phosphate
-
-
?
2.7.1.145
ATP + 2'-deoxyadenosine
Drosophila melanogaster
-
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
?
2.7.1.145
ATP + 2'-deoxycytidine
Drosophila melanogaster
-
ADP + 2'-deoxycytidine 5'-phosphate
-
-
?
2.7.1.145
ATP + 2'-deoxyguanosine
Drosophila melanogaster
-
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
?
2.7.1.145
ATP + 2'-deoxythymidine
Drosophila melanogaster
-
ADP + 2'-deoxythymidine 5'-phosphate
-
-
?
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
2.7.1.74
additional information
posttranscriptional regulation of the enzyme
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.113
enzyme activity is stabilized during purification in presence of Triton X-100 and ATP
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.74
colonic mucosa
-
Homo sapiens
-
2.7.1.74
kidney
very low enzyme expression
Homo sapiens
-
2.7.1.74
liver
very low enzyme expression
Homo sapiens
-
2.7.1.74
lymphoid tissue
high enzyme expression
Homo sapiens
-
2.7.1.74
additional information
tissue specific enzyme expression
Homo sapiens
-
2.7.1.74
muscle
very low enzyme expression
Homo sapiens
-
2.7.1.74
pancreas
very low enzyme expression
Homo sapiens
-
2.7.1.113
brain
-
Homo sapiens
-
2.7.1.113
liver
-
Homo sapiens
-
2.7.1.113
lymphoid tissue
-
Homo sapiens
-
2.7.1.113
skin
-
Homo sapiens
-
2.7.1.113
spleen
-
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.74
additional information
-
substrate specificity
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
ATP + 2',3'-dideoxycytidine
i.e. zalcitabine
661453
Homo sapiens
ADP + 2',3'-dideoxycytidine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + 2'-deoxyadenosine
-
661453
Homo sapiens
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + 2'-deoxycytidine
-
661453
Homo sapiens
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + 2'-deoxyguanosine
-
661453
Homo sapiens
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + 2-chloro-2'-deoxyadenosine
i.e. cladribine
661453
Homo sapiens
ADP + 2-chloro-2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + arabinosyl adenine
-
661453
Homo sapiens
ADP + arabinosyl adenine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + arabinosyl cytosine
i.e. cytosar
661453
Homo sapiens
ADP + arabinosyl cytosine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + beta-L-2',3'-dideoxy-3'-thiacytidine
-
661453
Homo sapiens
ADP + beta-L-2',3'-dideoxy-3'-thiacytidine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + difluoro-arabinofuranosyl adenine
i.e. fludarabine
661453
Homo sapiens
ADP + difluoro-arabinofuranosyl adenine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + difluorodeoxycytidine
i.e. gemcitabine
661453
Homo sapiens
ADP + difluorodeoxycytidine 5'-phosphate
-
-
-
?
2.7.1.74
additional information
substrate-enzyme interactions, overview, enzyme prefers nucleosides with sugars in the S-conformation, i.e C2'-endo-C3'-exo, binding of substrate induces conformational changes, the phosphate donor influences the acceptor substrate specificity, mechanism, overview
661453
Homo sapiens
?
-
-
-
-
2.7.1.74
UTP + 2',3'-dideoxycytidine
i.e. zalcitabine
661453
Homo sapiens
UDP + 2',3'-dideoxycytidine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + 2'-deoxyadenosine
-
661453
Homo sapiens
UDP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + 2'-deoxycytidine
-
661453
Homo sapiens
UDP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + 2'-deoxyguanosine
-
661453
Homo sapiens
UDP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + 2-chloro-2'-deoxyadenosine
i.e. cladribine
661453
Homo sapiens
UDP + 2-chloro-2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + arabinosyl adenine
-
661453
Homo sapiens
UDP + arabinosyl adenine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + arabinosyl cytosine
i.e. cytosar
661453
Homo sapiens
UDP + arabinosyl cytosine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + beta-L-2',3'-dideoxy-3'-thiacytidine
-
661453
Homo sapiens
UDP + beta-L-2',3'-dideoxy-3'-thiacytidine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + beta-L-dioxolane-cytidine
antitumor drug
661453
Homo sapiens
UDP + beta-L-dioxolane-cytidine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + difluoro-arabinofuranosyl adenine
i.e. fludarabine
661453
Homo sapiens
UDP + difluoro-arabinofuranosyl adenine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + difluorodeoxycytidine
i.e. gemcitabine
661453
Homo sapiens
UDP + difluorodeoxycytidine 5'-phosphate
-
-
-
?
2.7.1.113
ATP + 1-beta-D-arabinofuranosyladenine
-
661453
Homo sapiens
ADP + 1-beta-D-arabinofuranosyladenine 5'-phosphate
-
-
-
?
2.7.1.113
ATP + 2',2'-difluorodeoxyguanosine
-
661453
Homo sapiens
ADP + 2',2'-difluorodeoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.113
ATP + 2'-deoxyadenosine
-
661453
Homo sapiens
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.113
ATP + 2'-deoxyguanosine
-
661453
Homo sapiens
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.113
ATP + 2'-deoxyinosine
-
661453
Homo sapiens
ADP + 2'-deoxyinosine 5'-phosphate
-
-
-
?
2.7.1.113
ATP + 2-chloro-2'-arabinofluoro-2'-deoxyadenosine
-
661453
Homo sapiens
ADP + 2-chloro-2'-arabinofluoro-2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.113
ATP + 2-chlorodeoxyadenosine
-
661453
Homo sapiens
ADP + 2-chlorodeoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.113
ATP + 9-beta-D-arabinofuranosylguanine
-
661453
Homo sapiens
ADP + 9-beta-D-arabinofuranosylguanine 5'-phosphate
-
-
-
?
2.7.1.113
ATP + ganciclovir
i.e. 9-(1,3-dihydroxy-2-propoxymethyl)-guanine, low activity
661453
Homo sapiens
ADP + ganciclovir phosphate
-
-
-
?
2.7.1.113
ATP + penciclovir
i.e. 9-(2-hydroxy-1-hydroxymethyl-ethoxymethyl)-guanine, low activity
661453
Homo sapiens
ADP + penciclovir 5'-phosphate
-
-
-
?
2.7.1.113
additional information
substrate interactions, overview, enzyme shows relaxed enantioselectivity, accepting D- and L-enantiomers of beta-deoxyguanosine but with lower activity than alpha-L-deoxyguanosine, and beta-L-deoxyadenosine, enzyme accepts purine deoxynucleosides and analogues as substrates utilizing a triphosphate donor substrate
661453
Homo sapiens
?
-
-
-
-
2.7.1.113
UTP + 2'-deoxyadenosine
-
661453
Homo sapiens
UDP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.113
UTP + 2'-deoxyguanosine
-
661453
Homo sapiens
UDP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.113
UTP + 2'-deoxyinosine
-
661453
Homo sapiens
UDP + 2'-deoxyinosine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 1-beta-D-arabinofuranosylcytosine
-
661453
Drosophila melanogaster
ADP + 1-beta-D-arabinofuranosylcytosine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 1-beta-D-arabinofuranosylthymine
-
661453
Drosophila melanogaster
ADP + 1-beta-D-arabinofuranosylthymine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxyadenosine
-
661453
Drosophila melanogaster
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxycytidine
-
661453
Drosophila melanogaster
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxyguanosine
-
661453
Drosophila melanogaster
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxythymidine
-
661453
Drosophila melanogaster
ADP + 2'-deoxythymidine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2-chloro-2'-deoxyadenosine
-
661453
Drosophila melanogaster
ADP + 2-chloro-2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 5-fluoro-2'-deoxyuridine
-
661453
Drosophila melanogaster
ADP + 5-fluoro-2'-deoxyuridine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + E-5-(2-bromovinyl)-2'-deoxyuridine
-
661453
Drosophila melanogaster
ADP + E-5-(2-bromovinyl)-2'-deoxyuridine phosphate
-
-
-
?
2.7.1.145
additional information
broad substrate specificity
661453
Drosophila melanogaster
?
-
-
-
-
2.7.1.145
additional information
substrate specificity, no activity with purine ribonucleosides and dideoxynucleosides
661453
Bombyx mori
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.1.74
More
3D-structure analysis, dCK structure model, overview
Homo sapiens
2.7.1.113
dimer
2 * 30000
Homo sapiens
2.7.1.113
More
ternary and quarternary structure analysis, overview
Homo sapiens
2.7.1.145
dimer
-
Bombyx mori
2.7.1.145
dimer
-
Drosophila melanogaster
2.7.1.145
More
ternary and quarternary structure analysis, overview
Bombyx mori
2.7.1.145
More
ternary and quarternary structure analysis, overview
Drosophila melanogaster