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Literature summary extracted from

  • Samsonova, N.N.; Smirnov, S.V.; Altman, I.B.; Ptitsyn, L.R.
    Molecular cloning and characterization of Escherichia coli K12 ygjG gene (2003), BMC Microbiol., 3, 2.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.82 expression in Escherichia coli TG1 and BL21 (DE3) Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.82 9.2
-
 putrescine
-
 Escherichia coli
2.6.1.82 19
-
 2-oxoglutaric acid
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.82 52000
-
 SDS-PAGE, 49600 Da for ORF2 coding protein and 2100 Da for His6-tag leader peptide Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.82 Escherichia coli P42588 K12
-
2.6.1.82 Escherichia coli P42588 MG1655 (K-12 lambda-F-)
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.82 95% purified by immobilized-metal affinity chromatography Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
2.6.1.82 4-aminobutanal = 1-pyrroline + H2O (1b) Escherichia coli
a
2.6.1.82 putrescine + 2-oxoglutarate = 4-aminobutanal + L-glutamate (1a) Escherichia coli
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.82 0.047
-
 highest activity in cell extracts grown under nitrogen limitation Escherichia coli
2.6.1.82 2.16
-
 essential level of glutamate formation activity in crude cells of BL21(DE3) harboring pET-Ht-ORF2 Escherichia coli
2.6.1.82 11.68
-
 purified enzyme with yield of 81% Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.6.1.82 -70°C, phosphate buffer, pH 7.4, 15% glycerol, until required Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.82 agmatine + 2-oxoglutarate 2% activity Escherichia coli ?
-
 ?
2.6.1.82 alpha-ketobutyric acid + putrescine 38% activity Escherichia coli L-aspartate + 4-aminobutanal
-
 ?
2.6.1.82 cadaverine + 2-oxoglutarate 97% activity Escherichia coli L-glutamate + 5-aminopentanal
-
 ?
2.6.1.82 L-ornithine + 2-oxoglutarate 2% activity Escherichia coli ?
-
 ?
2.6.1.82 putrescine + 2-oxoglutarate putrescine best amino group donor for the enzyme, 100% activity Escherichia coli L-glutamate + 4-aminobutanal
-
 ?
2.6.1.82 pyruvate + putrescine 12% activity Escherichia coli L-alanine + 4-aminobutanal
-
 ?
2.6.1.82 spermidine + 2-oxoglutarate 32% activity Escherichia coli ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.6.1.82 PATase
-
 Escherichia coli
2.6.1.82 YgjG
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.82 60
-
-
 Escherichia coli

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.6.1.82 20 80
-
 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.82 9
-
 in Tris-HCl buffer Escherichia coli