BRENDA - Enzyme Database

Molecular cloning and characterization of Escherichia coli K12 ygjG gene

Samsonova, N.N.; Smirnov, S.V.; Altman, I.B.; Ptitsyn, L.R.; BMC Microbiol. 3, 2 (2003)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.6.1.82
expression in Escherichia coli TG1 and BL21 (DE3)
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.6.1.82
9.2
-
putrescine
-
Escherichia coli
2.6.1.82
19
-
2-oxoglutaric acid
-
Escherichia coli
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.6.1.82
52000
-
SDS-PAGE, 49600 Da for ORF2 coding protein and 2100 Da for His6-tag leader peptide
Escherichia coli
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.6.1.82
Escherichia coli
P42588
K12; MG1655 (K-12 lambda-F-)
-
Purification (Commentary)
EC Number
Commentary
Organism
2.6.1.82
95% purified by immobilized-metal affinity chromatography
Escherichia coli
Reaction
EC Number
Reaction
Commentary
Organism
2.6.1.82
4-aminobutanal = 1-pyrroline + H2O
(1b)
Escherichia coli
2.6.1.82
putrescine + 2-oxoglutarate = 4-aminobutanal + L-glutamate
(1a)
Escherichia coli
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.6.1.82
0.047
-
highest activity in cell extracts grown under nitrogen limitation
Escherichia coli
2.6.1.82
2.16
-
essential level of glutamate formation activity in crude cells of BL21(DE3) harboring pET-Ht-ORF2
Escherichia coli
2.6.1.82
11.68
-
purified enzyme with yield of 81%
Escherichia coli
Storage Stability
EC Number
Storage Stability
Organism
2.6.1.82
-70°C, phosphate buffer, pH 7.4, 15% glycerol, until required
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.82
agmatine + 2-oxoglutarate
2% activity
661399
Escherichia coli
?
-
-
-
?
2.6.1.82
alpha-ketobutyric acid + putrescine
38% activity
661399
Escherichia coli
L-aspartate + 4-aminobutanal
-
-
-
?
2.6.1.82
cadaverine + 2-oxoglutarate
97% activity
661399
Escherichia coli
L-glutamate + 5-aminopentanal
-
-
-
?
2.6.1.82
L-ornithine + 2-oxoglutarate
2% activity
661399
Escherichia coli
?
-
-
-
?
2.6.1.82
putrescine + 2-oxoglutarate
putrescine best amino group donor for the enzyme, 100% activity
661399
Escherichia coli
L-glutamate + 4-aminobutanal
-
-
-
?
2.6.1.82
pyruvate + putrescine
12% activity
661399
Escherichia coli
L-alanine + 4-aminobutanal
-
-
-
?
2.6.1.82
spermidine + 2-oxoglutarate
32% activity
661399
Escherichia coli
?
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.6.1.82
60
-
-
Escherichia coli
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
2.6.1.82
20
80
-
Escherichia coli
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.6.1.82
9
-
in Tris-HCl buffer
Escherichia coli
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.6.1.82
expression in Escherichia coli TG1 and BL21 (DE3)
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.6.1.82
9.2
-
putrescine
-
Escherichia coli
2.6.1.82
19
-
2-oxoglutaric acid
-
Escherichia coli
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.6.1.82
52000
-
SDS-PAGE, 49600 Da for ORF2 coding protein and 2100 Da for His6-tag leader peptide
Escherichia coli
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.6.1.82
95% purified by immobilized-metal affinity chromatography
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.6.1.82
0.047
-
highest activity in cell extracts grown under nitrogen limitation
Escherichia coli
2.6.1.82
2.16
-
essential level of glutamate formation activity in crude cells of BL21(DE3) harboring pET-Ht-ORF2
Escherichia coli
2.6.1.82
11.68
-
purified enzyme with yield of 81%
Escherichia coli
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.6.1.82
-70°C, phosphate buffer, pH 7.4, 15% glycerol, until required
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.82
agmatine + 2-oxoglutarate
2% activity
661399
Escherichia coli
?
-
-
-
?
2.6.1.82
alpha-ketobutyric acid + putrescine
38% activity
661399
Escherichia coli
L-aspartate + 4-aminobutanal
-
-
-
?
2.6.1.82
cadaverine + 2-oxoglutarate
97% activity
661399
Escherichia coli
L-glutamate + 5-aminopentanal
-
-
-
?
2.6.1.82
L-ornithine + 2-oxoglutarate
2% activity
661399
Escherichia coli
?
-
-
-
?
2.6.1.82
putrescine + 2-oxoglutarate
putrescine best amino group donor for the enzyme, 100% activity
661399
Escherichia coli
L-glutamate + 4-aminobutanal
-
-
-
?
2.6.1.82
pyruvate + putrescine
12% activity
661399
Escherichia coli
L-alanine + 4-aminobutanal
-
-
-
?
2.6.1.82
spermidine + 2-oxoglutarate
32% activity
661399
Escherichia coli
?
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.6.1.82
60
-
-
Escherichia coli
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
2.6.1.82
20
80
-
Escherichia coli
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.6.1.82
9
-
in Tris-HCl buffer
Escherichia coli