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Literature summary extracted from

  • Van Arsdell, S.W.; Perkins, J.B.; Yocum, R.R.; Luan, L.; Howitt, C.L.; Chatterjee, N.P.; Pero, J.G.
    Removing a bottleneck in the Bacillus subtilis biotin pathway: bioA utilizes lysine rather than S-adenosylmethionine as the amino donor in the KAPA-to-DAPA reaction (2005), Biotechnol. Bioeng., 91, 75-83.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.6.1.62 biotechnology evaluation of biotin overproduction, biotin production by Bacillus subtilis fermentation can be optimized by addition of exogenous lysine or mutational deregulation of lysine in the producing strain Bacillus subtilis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.62 expression in strain PY79 Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.62 8-Amino-7-oxononanoate substrate inhibition above 0.08 mM Bacillus subtilis
2.6.1.105 8-Amino-7-oxononanoate
-
 Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.62 additional information
-
 additional information kinetics Bacillus subtilis
2.6.1.105 2
-
 L-lysine pH 8.3, 37°C Bacillus subtilis
2.6.1.105 25
-
 8-Amino-7-oxononanoate pH 8.3, 37°C Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.62 DL-lysine + 8-amino-7-oxononanoate Bacillus subtilis L-lysine is preferred ? + (7S,8R)-diaminononanoate
-
 ?
2.6.1.105 L-lysine + 8-amino-7-oxononanoate Bacillus subtilis the enzyme participates in the pathway for biotin biosynthesis (S)-2-amino-6-oxohexanoate + 7,8-diaminononanoate (S)-2-amino-6-oxohexanoate i.e. L-2-aminoadipate 6-semialdehyde i.e. L-allysine ?
2.6.1.105 L-lysine + 8-amino-7-oxononanoate Bacillus subtilis 168 the enzyme participates in the pathway for biotin biosynthesis (S)-2-amino-6-oxohexanoate + 7,8-diaminononanoate (S)-2-amino-6-oxohexanoate i.e. L-2-aminoadipate 6-semialdehyde i.e. L-allysine ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.62 Bacillus subtilis
-
 gene bioA, strains BI282 and BI603, no activity in strain BI9
-
2.6.1.105 Bacillus subtilis P53555
-
-
2.6.1.105 Bacillus subtilis 168 P53555
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.6.1.62 S-adenosyl-L-methionine + 8-amino-7-oxononanoate = S-adenosyl-4-(methylsulfanyl)-2-oxobutanoate + 7,8-diaminononanoate double displacement or ping pong reaction mechanism Bacillus subtilis
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.62 additional information
-
 bioindicator assay method, overview Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.62 DL-lysine + 8-amino-7-oxononanoate L-lysine is preferred Bacillus subtilis ? + (7S,8R)-diaminononanoate
-
 ?
2.6.1.62 additional information enzyme does not utilize S-adenosyl-L-methionine, Nalpha-acetyl-L-lysine, Nepsilon-acetyl-L-lysine, Nepsilon-methyl-L-lysine, glycine-lysine dipeptide, lysine-glycine dipeptide, and diaminopimelic acid as amino donors Bacillus subtilis ?
-
 ?
2.6.1.62 S-2-aminoethyl-L-cysteine + 8-amino-7-oxononanoate
-
 Bacillus subtilis ? + (7S,8R)-diaminononanoate
-
 ?
2.6.1.105 L-lysine + 8-amino-7-oxononanoate the enzyme participates in the pathway for biotin biosynthesis Bacillus subtilis (S)-2-amino-6-oxohexanoate + 7,8-diaminononanoate (S)-2-amino-6-oxohexanoate i.e. L-2-aminoadipate 6-semialdehyde i.e. L-allysine ?
2.6.1.105 L-lysine + 8-amino-7-oxononanoate higly specific for L-lysine as amino donor Bacillus subtilis (S)-2-amino-6-oxohexanoate + 7,8-diaminononanoate (S)-2-amino-6-oxohexanoate i.e. L-2-aminoadipate 6-semialdehyde i.e. L-allysine ?
2.6.1.105 L-lysine + 8-amino-7-oxononanoate the enzyme participates in the pathway for biotin biosynthesis Bacillus subtilis 168 (S)-2-amino-6-oxohexanoate + 7,8-diaminononanoate (S)-2-amino-6-oxohexanoate i.e. L-2-aminoadipate 6-semialdehyde i.e. L-allysine ?
2.6.1.105 L-lysine + 8-amino-7-oxononanoate higly specific for L-lysine as amino donor Bacillus subtilis 168 (S)-2-amino-6-oxohexanoate + 7,8-diaminononanoate (S)-2-amino-6-oxohexanoate i.e. L-2-aminoadipate 6-semialdehyde i.e. L-allysine ?

Synonyms

EC Number Synonyms Comment Organism
2.6.1.62 DAPA amino transferase
-
 Bacillus subtilis
2.6.1.105 bioA gene name, ambiguous Bacillus subtilis
2.6.1.105 bioK
-
 Bacillus subtilis
2.6.1.105 DAPA aminotransferase ambiguous Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.62 37
-
 assay at Bacillus subtilis
2.6.1.105 37
-
 assay at Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.62 8.6
-
-
 Bacillus subtilis
2.6.1.105 8.3
-
 assay at Bacillus subtilis

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.62 pyridoxal 5'-phosphate
-
 Bacillus subtilis
2.6.1.105 pyridoxal 5'-phosphate
-
 Bacillus subtilis

General Information

EC Number General Information Comment Organism
2.6.1.105 physiological function the enzyme participates in the pathway for biotin biosynthesis Bacillus subtilis