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Literature summary extracted from

  • Haas, A.L.; Laun, N.P.; Begley, T.P.
    Thi20, a remarkable enzyme from Saccharomyces cerevisiae with dual thiamin biosynthetic and degradation activities (2005), Bioorg. Chem., 33, 338-344.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.49 expression in Escherichia coli Saccharomyces cerevisiae
2.7.4.7 expression in Escherichia coli Saccharomyces cerevisiae
3.5.99.2 expression in Escherichia coli Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.49 ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine Saccharomyces cerevisiae
-
 ADP + 4-amino-5-phosphonooxymethyl-2-methylpyrimidine
-
 ?
2.7.4.7 ATP + (4-amino-2-methylpyrimidin-5-yl)methyl phosphate Saccharomyces cerevisiae
-
 ADP + (4-amino-2-methylpyrimidin-5-yl)methyl diphosphate
-
 ?
3.5.99.2 thiamine + H2O Saccharomyces cerevisiae
-
 4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.49 Saccharomyces cerevisiae Q08224 trifunctional enzyme EC 3.5.99.2/EC 2.7.1.49/EC 2.7.4.7
-
2.7.4.7 Saccharomyces cerevisiae Q08224 trifunctional enzyme EC 3.5.99.2/EC 2.7.1.49/EC 2.7.4.7
-
3.5.99.2 Saccharomyces cerevisiae Q08224 trifunctional enzyme EC 3.5.99.2/EC 2.7.1.49/EC 2.7.4.7
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.49
-
 Saccharomyces cerevisiae
2.7.4.7
-
 Saccharomyces cerevisiae
3.5.99.2
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.49 ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine
-
 Saccharomyces cerevisiae ADP + 4-amino-5-phosphonooxymethyl-2-methylpyrimidine
-
 ?
2.7.4.7 ATP + (4-amino-2-methylpyrimidin-5-yl)methyl phosphate
-
 Saccharomyces cerevisiae ADP + (4-amino-2-methylpyrimidin-5-yl)methyl diphosphate
-
 ?
3.5.99.2 thiamine + H2O
-
 Saccharomyces cerevisiae 4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.49 Thi20
-
 Saccharomyces cerevisiae
2.7.4.7 Thi20
-
 Saccharomyces cerevisiae
3.5.99.2 Thi20
-
 Saccharomyces cerevisiae
3.5.99.2 thiaminase II
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.49 32
-
 assay at Saccharomyces cerevisiae
2.7.4.7 32
-
 assay at Saccharomyces cerevisiae
3.5.99.2 22
-
 assay at Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.49 8
-
 assay at Saccharomyces cerevisiae
2.7.4.7 8
-
 assay at Saccharomyces cerevisiae
3.5.99.2 6.6
-
 assay at Saccharomyces cerevisiae