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Literature summary extracted from

  • Fresquet, V.; Thoden, J.B.; Holden, H.M.; Raushel, F.M.
    Kinetic mechanism of asparagine synthetase from Vibrio cholerae (2004), Bioorg. Chem., 32, 63-75.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.5.4 expression in Escherichia coli Vibrio cholerae serotype O1

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.5.4 alpha,beta-methylene-ATP
-
 Vibrio cholerae serotype O1
6.3.5.4 Asn
-
 Vibrio cholerae serotype O1
6.3.5.4 diphosphate
-
 Vibrio cholerae serotype O1
6.3.5.4 L-cysteinesulfinic acid
-
 Vibrio cholerae serotype O1
6.3.5.4 L-glutamic acid gamma-methyl ester
-
 Vibrio cholerae serotype O1

Organism

EC Number Organism UniProt Comment Textmining
6.3.5.4 Vibrio cholerae serotype O1
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.5.4 ATP + L-aspartate + L-glutamine the reaction sequence begins with the ordered addition of ATP and aspartate. Diphosphate is released, followed by the addition of ammonia and the release of asparagine and AMP. Glutamine is simultaneously hydrolyzed at a second site and the ammonia intermediate diffuses through an interdomain protein tunnel from the site of production to the site of utilization. The data are also consistent with the dead-end binding of asparagine to the glutamine binding site and diphosphate with free enzyme. The rate of hydrolysis of glutamine is largely independent of the activation of aspartate and thus the reaction rates at the two active sites are essentially uncoupled from one another Vibrio cholerae serotype O1 AMP + diphosphate + L-asparagine + L-glutamate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.5.4 AsnB
-
 Vibrio cholerae serotype O1
6.3.5.4 Asparagine synthetase B
-
 Vibrio cholerae serotype O1

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.5.4 5.8
-
 ATP
-
 Vibrio cholerae serotype O1
6.3.5.4 5.8
-
 L-asparagine
-
 Vibrio cholerae serotype O1
6.3.5.4 5.8
-
 glutamine
-
 Vibrio cholerae serotype O1

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
6.3.5.4 0.021
-
 diphosphate Kis, versus ATP Vibrio cholerae serotype O1
6.3.5.4 0.023
-
 Asn Kis, versus Gln Vibrio cholerae serotype O1
6.3.5.4 0.65
-
 diphosphate Kii, versus Asp Vibrio cholerae serotype O1
6.3.5.4 0.85
-
 diphosphate Kii, versus NH3 or versus Gln Vibrio cholerae serotype O1
6.3.5.4 0.85
-
 diphosphate Kis, versus Asp Vibrio cholerae serotype O1
6.3.5.4 1.1
-
 L-cysteine sulfinic acid Kis, versus Asp Vibrio cholerae serotype O1
6.3.5.4 1.1
-
 alpha,beta-methylene-ATP Kis, versus ATP Vibrio cholerae serotype O1
6.3.5.4 2.2
-
 L-cysteine sulfinic acid Kii, versus NH3 Vibrio cholerae serotype O1
6.3.5.4 2.5
-
 L-cysteine sulfinic acid Kii, versus ATP Vibrio cholerae serotype O1
6.3.5.4 2.6
-
 alpha,beta-methylene-ATP Kis or Kii, versus Asp Vibrio cholerae serotype O1
6.3.5.4 3.2
-
 alpha,beta-methylene-ATP Kii, versus Gln Vibrio cholerae serotype O1
6.3.5.4 3.7
-
 alpha,beta-methylene-ATP Kii, versus NH3 Vibrio cholerae serotype O1
6.3.5.4 6.4
-
 L-cysteine sulfinic acid Kii, versus Gln Vibrio cholerae serotype O1
6.3.5.4 6.5
-
 L-glutamic acid gamma-methyl ester Kis, versus NH3 Vibrio cholerae serotype O1
6.3.5.4 7.2
-
 L-cysteine sulfinic acid Kis, versus Gln Vibrio cholerae serotype O1
6.3.5.4 8.9
-
 L-glutamic acid gamma-methyl ester Kis, versus Gln Vibrio cholerae serotype O1
6.3.5.4 27
-
 L-glutamic acid gamma-methyl ester Kii, versus Asp Vibrio cholerae serotype O1
6.3.5.4 29
-
 L-glutamic acid gamma-methyl ester Kii, versus ATP Vibrio cholerae serotype O1