EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.65 | expression in Spodoptera frugiperda (Sf9) insect cells | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.65 | 0.7 | - |
Gal-beta-1,3GlcNAcO(CH2)3NHCO(CH2)5NH-biotin | at pH 7.0 and presence of Mn2+ | Homo sapiens | |
2.4.1.65 | 0.9 | - |
Gal-beta-1,3GlcNAcO(CH2)3NHCO(CH2)5NH-biotin | at pH 4.7 and absence of Mn2+ | Homo sapiens | |
2.4.1.65 | 1.5 | - |
Gal-beta-1,3GlcNAcO(CH2)3NHCO(CH2)5NH-biotin | at pH 4.7 and presence of Mn2+ | Homo sapiens | |
2.4.1.65 | 3.3 | - |
Gal-beta-1,3GlcNAcO(CH2)3NHCO(CH2)5NH-biotin | at pH 7.0 and absence of Mn2+ | Homo sapiens |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.65 | Ca2+ | leads to 2.1fold activation of SFT3 | Homo sapiens | |
2.4.1.65 | Co2+ | leads to 2.8fold activation of SFT3 | Homo sapiens | |
2.4.1.65 | Cu2+ | no significant activity is detected | Homo sapiens | |
2.4.1.65 | Mg2+ | leads to 2.5fold activation of SFT3 | Homo sapiens | |
2.4.1.65 | Mn2+ | binds the enzyme and increases affinity for the acceptor. One possible functional role of manganese in catalysis can be as an electrophilic catalyst, co-ordinating the negative charges of the phosphate groups of the GDP-Fuc donor and promoting Fuc transfer. A low pH values such role would be played by the proton. Mn2+ leads to 2.7fold activation of SFT3 | Homo sapiens | |
2.4.1.65 | additional information | the enzyme retains approximately 35% of its maximal activity in the absence of metal ions | Homo sapiens | |
2.4.1.65 | Zn2+ | leads to 80% inhibition of STF3 | Homo sapiens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.65 | Homo sapiens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.65 | anion exchange-chromatography on a CM-Sepharose column, followed by an affinity chromatography on a GDP Fractogel column | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.65 | GDP-fucose + Gal-beta-1,3GlcNAcO(CH2)3NHCO(CH2)5NH-biotin | - |
Homo sapiens | GDP + Gal-beta-1,3[alpha-L-fucosyl-(1,4)]GlcNAcO(CH2)3NHCO(CH2)5NH-biotin | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.65 | alpha-3/4 fucosyltransferase III | - |
Homo sapiens |
2.4.1.65 | SFT3 | - |
Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.65 | 0.1 | - |
Gal-beta-1,3GlcNAcO(CH2)3NHCO(CH2)5NH-biotin | at pH 7.0 and absence of Mn2+ | Homo sapiens | |
2.4.1.65 | 0.2 | - |
Gal-beta-1,3GlcNAcO(CH2)3NHCO(CH2)5NH-biotin | at pH 4.7 and presence of Mn2+ | Homo sapiens | |
2.4.1.65 | 0.2 | - |
Gal-beta-1,3GlcNAcO(CH2)3NHCO(CH2)5NH-biotin | at pH 7.0 and presence of Mn2+ | Homo sapiens | |
2.4.1.65 | 0.3 | - |
Gal-beta-1,3GlcNAcO(CH2)3NHCO(CH2)5NH-biotin | at pH 4.7 and absence of Mn2+ | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.65 | 4.5 | - |
in the absence of Mn2+ | Homo sapiens |
2.4.1.65 | 7 | - |
in the presence of Mn2+ | Homo sapiens |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.65 | 6 | 8 | in the presence of Mn2+ | Homo sapiens |