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Literature summary extracted from

  • Palma, A.S.; Morais, V.A.; Coelho, A.V.; Costa, J.
    Effect of the manganese ion on human alpha3/4 fucosyltransferase III activity (2004), BioMetals, 17, 35-43.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.65 expression in Spodoptera frugiperda (Sf9) insect cells Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.65 0.7
-
Gal-beta-1,3GlcNAcO(CH2)3NHCO(CH2)5NH-biotin at pH 7.0 and presence of Mn2+ Homo sapiens
2.4.1.65 0.9
-
Gal-beta-1,3GlcNAcO(CH2)3NHCO(CH2)5NH-biotin at pH 4.7 and absence of Mn2+ Homo sapiens
2.4.1.65 1.5
-
Gal-beta-1,3GlcNAcO(CH2)3NHCO(CH2)5NH-biotin at pH 4.7 and presence of Mn2+ Homo sapiens
2.4.1.65 3.3
-
Gal-beta-1,3GlcNAcO(CH2)3NHCO(CH2)5NH-biotin at pH 7.0 and absence of Mn2+ Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.65 Ca2+ leads to 2.1fold activation of SFT3 Homo sapiens
2.4.1.65 Co2+ leads to 2.8fold activation of SFT3 Homo sapiens
2.4.1.65 Cu2+ no significant activity is detected Homo sapiens
2.4.1.65 Mg2+ leads to 2.5fold activation of SFT3 Homo sapiens
2.4.1.65 Mn2+ binds the enzyme and increases affinity for the acceptor. One possible functional role of manganese in catalysis can be as an electrophilic catalyst, co-ordinating the negative charges of the phosphate groups of the GDP-Fuc donor and promoting Fuc transfer. A low pH values such role would be played by the proton. Mn2+ leads to 2.7fold activation of SFT3 Homo sapiens
2.4.1.65 additional information the enzyme retains approximately 35% of its maximal activity in the absence of metal ions Homo sapiens
2.4.1.65 Zn2+ leads to 80% inhibition of STF3 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.65 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.65 anion exchange-chromatography on a CM-Sepharose column, followed by an affinity chromatography on a GDP Fractogel column Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.65 GDP-fucose + Gal-beta-1,3GlcNAcO(CH2)3NHCO(CH2)5NH-biotin
-
Homo sapiens GDP + Gal-beta-1,3[alpha-L-fucosyl-(1,4)]GlcNAcO(CH2)3NHCO(CH2)5NH-biotin
-
?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.65 alpha-3/4 fucosyltransferase III
-
Homo sapiens
2.4.1.65 SFT3
-
Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.65 0.1
-
Gal-beta-1,3GlcNAcO(CH2)3NHCO(CH2)5NH-biotin at pH 7.0 and absence of Mn2+ Homo sapiens
2.4.1.65 0.2
-
Gal-beta-1,3GlcNAcO(CH2)3NHCO(CH2)5NH-biotin at pH 4.7 and presence of Mn2+ Homo sapiens
2.4.1.65 0.2
-
Gal-beta-1,3GlcNAcO(CH2)3NHCO(CH2)5NH-biotin at pH 7.0 and presence of Mn2+ Homo sapiens
2.4.1.65 0.3
-
Gal-beta-1,3GlcNAcO(CH2)3NHCO(CH2)5NH-biotin at pH 4.7 and absence of Mn2+ Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.65 4.5
-
in the absence of Mn2+ Homo sapiens
2.4.1.65 7
-
in the presence of Mn2+ Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.65 6 8 in the presence of Mn2+ Homo sapiens