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Literature summary extracted from

  • Yamamoto, S.; Mutoh, N.; Ikai, H.; Nagasaka, M.
    Occurrence of a novel L-2,4-diaminobutyrate decarboxylase activity in some species of Enterobacteriaceae, and purification and characterization of the enzymes of Enterobacter aerogenes and Serratia marcescens (1996), Biol. Pharm. Bull., 19, 1298-1303.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.1.86 additional information no activation by ATP or GTP Klebsiella aerogenes
4.1.1.86 additional information no activation by ATP or GTP Serratia marcescens

General Stability

EC Number General Stability Organism
4.1.1.86 freezing and thawing of purified enzyme causes severe loss of activity Klebsiella aerogenes
4.1.1.86 freezing and thawing of purified enzyme causes severe loss of activity Serratia marcescens

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.86 Co2+
-
 Klebsiella aerogenes
4.1.1.86 Co2+
-
 Serratia marcescens
4.1.1.86 Mn2+
-
 Klebsiella aerogenes
4.1.1.86 Mn2+
-
 Serratia marcescens
4.1.1.86 Ni2+
-
 Klebsiella aerogenes
4.1.1.86 Ni2+
-
 Serratia marcescens
4.1.1.86 Zn2+
-
 Klebsiella aerogenes
4.1.1.86 Zn2+
-
 Serratia marcescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.86 0.3
-
 Mg2+ pH 8.0, 37°C Klebsiella aerogenes
4.1.1.86 0.32
-
 L-2,4-diaminobutanoate pH 8.0, 37°C Serratia marcescens
4.1.1.86 1.07
-
 L-2,4-diaminobutanoate pH 8.0, 37°C Klebsiella aerogenes
4.1.1.86 1.2
-
 Mg2+ pH 8.0, 37°C Serratia marcescens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.86 Ca2+ about 65% of the activity with Mg2+ Klebsiella aerogenes
4.1.1.86 Ca2+ about 65% of the activity with Mg2+ Serratia marcescens
4.1.1.86 Mg2+ required, activates 7fold at 10 mM Klebsiella aerogenes
4.1.1.86 Mg2+ required, activates 17fold at 10 mM Serratia marcescens
4.1.1.86 Sr2+ about 65% of the activity with Mg2+ Klebsiella aerogenes
4.1.1.86 Sr2+ about 65% of the activity with Mg2+ Serratia marcescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.86 51000
-
 2 * 51000, SDS-PAGE Klebsiella aerogenes
4.1.1.86 51000
-
 2 * 51000, SDS-PAGE Serratia marcescens
4.1.1.86 100000
-
 gel filtration Klebsiella aerogenes
4.1.1.86 100000
-
 gel filtration Serratia marcescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.86 L-2,4-diaminobutanoate Klebsiella aerogenes
-
 propane-1,3-diamine + CO2
-
 ?
4.1.1.86 L-2,4-diaminobutanoate Serratia marcescens
-
 propane-1,3-diamine + CO2
-
 ?
4.1.1.86 L-2,4-diaminobutanoate Enterobacter cloacae
-
 propane-1,3-diamine + CO2
-
 ?
4.1.1.86 L-2,4-diaminobutanoate Pantoea agglomerans
-
 propane-1,3-diamine + CO2
-
 ?
4.1.1.86 L-2,4-diaminobutanoate Serratia liquefaciens
-
 propane-1,3-diamine + CO2
-
 ?
4.1.1.86 L-2,4-diaminobutanoate Klebsiella pneumoniae
-
 propane-1,3-diamine + CO2
-
 ?
4.1.1.86 L-2,4-diaminobutanoate Klebsiella oxytoca
-
 propane-1,3-diamine + CO2
-
 ?
4.1.1.86 L-2,4-diaminobutanoate Citrobacter freundii
-
 propane-1,3-diamine + CO2
-
 ?
4.1.1.86 L-2,4-diaminobutanoate Serratia liquefaciens RM 2-Ia
-
 propane-1,3-diamine + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.86 Citrobacter freundii
-
 strain ATCC 8090
-
4.1.1.86 Enterobacter cloacae
-
 strain ATCC 13047
-
4.1.1.86 Klebsiella aerogenes
-
 strain ATCC 13048
-
4.1.1.86 Klebsiella oxytoca
-
 strain ATCC 13182
-
4.1.1.86 Klebsiella pneumoniae
-
 strain ATCC 13882
-
4.1.1.86 Pantoea agglomerans
-
 strain ATCC 27155
-
4.1.1.86 Serratia liquefaciens
-
-
-
4.1.1.86 Serratia liquefaciens RM 2-Ia
-
-
-
4.1.1.86 Serratia marcescens
-
 strain ATCC 13880
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.86 native enzyme 1750fold to homogeneity by two steps of ion exchange chromatography, gel filtration, hydroxyapatite chromatography, and another two steps of ion exchange chromatography Klebsiella aerogenes
4.1.1.86 native enzyme 886fold to homogeneity by two steps of ion exchange chromatography, gel filtration, hydroxyapatite chromatography, and another two steps of ion exchange chromatography Serratia marcescens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.86 0.0022
-
 enzyme in cell extract Citrobacter freundii
4.1.1.86 0.0039
-
 enzyme in cell extract Pantoea agglomerans
4.1.1.86 0.00392
-
 enzyme in cell extract Serratia liquefaciens
4.1.1.86 0.0042
-
 enzyme in cell extract Klebsiella oxytoca
4.1.1.86 0.005
-
 enzyme in cell extract Klebsiella aerogenes
4.1.1.86 0.0051
-
 enzyme in cell extract Serratia marcescens
4.1.1.86 0.0052
-
 enzyme in cell extract Enterobacter cloacae
4.1.1.86 0.0071
-
 enzyme in cell extract Klebsiella pneumoniae
4.1.1.86 3.9
-
 purified enzyme Serratia marcescens
4.1.1.86 11.8
-
 purified enzyme Klebsiella aerogenes

Storage Stability

EC Number Storage Stability Organism
4.1.1.86 1°C, purified enzyme, 20 mM potassium phosphate, pH 7.5, 0.04 mM pyridoxal 5'-phosphate, and 0.02% NaN3, 2 weeks without loss of activity Klebsiella aerogenes
4.1.1.86 1°C, purified enzyme, 20 mM potassium phosphate, pH 7.5, 0.04 mM pyridoxal 5'-phosphate, and 0.02% NaN3, loss of 65% activity within 1 week Serratia marcescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.86 L-2,4-diaminobutanoate
-
 Klebsiella aerogenes propane-1,3-diamine + CO2
-
 ?
4.1.1.86 L-2,4-diaminobutanoate
-
 Serratia marcescens propane-1,3-diamine + CO2
-
 ?
4.1.1.86 L-2,4-diaminobutanoate
-
 Enterobacter cloacae propane-1,3-diamine + CO2
-
 ?
4.1.1.86 L-2,4-diaminobutanoate
-
 Pantoea agglomerans propane-1,3-diamine + CO2
-
 ?
4.1.1.86 L-2,4-diaminobutanoate
-
 Serratia liquefaciens propane-1,3-diamine + CO2
-
 ?
4.1.1.86 L-2,4-diaminobutanoate
-
 Klebsiella pneumoniae propane-1,3-diamine + CO2
-
 ?
4.1.1.86 L-2,4-diaminobutanoate
-
 Klebsiella oxytoca propane-1,3-diamine + CO2
-
 ?
4.1.1.86 L-2,4-diaminobutanoate
-
 Citrobacter freundii propane-1,3-diamine + CO2
-
 ?
4.1.1.86 L-2,4-diaminobutanoate
-
 Serratia liquefaciens RM 2-Ia propane-1,3-diamine + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.86 dimer 2 * 51000, SDS-PAGE Klebsiella aerogenes
4.1.1.86 dimer 2 * 51000, SDS-PAGE Serratia marcescens

Synonyms

EC Number Synonyms Comment Organism
4.1.1.86 DABA DC
-
 Klebsiella aerogenes
4.1.1.86 DABA DC
-
 Serratia marcescens
4.1.1.86 DABA DC
-
 Enterobacter cloacae
4.1.1.86 DABA DC
-
 Pantoea agglomerans
4.1.1.86 DABA DC
-
 Serratia liquefaciens
4.1.1.86 DABA DC
-
 Klebsiella pneumoniae
4.1.1.86 DABA DC
-
 Klebsiella oxytoca
4.1.1.86 DABA DC
-
 Citrobacter freundii
4.1.1.86 L-2,4-diaminobutyrate decarboxylase
-
 Klebsiella aerogenes
4.1.1.86 L-2,4-diaminobutyrate decarboxylase
-
 Serratia marcescens
4.1.1.86 L-2,4-diaminobutyrate decarboxylase
-
 Enterobacter cloacae
4.1.1.86 L-2,4-diaminobutyrate decarboxylase
-
 Pantoea agglomerans
4.1.1.86 L-2,4-diaminobutyrate decarboxylase
-
 Serratia liquefaciens
4.1.1.86 L-2,4-diaminobutyrate decarboxylase
-
 Klebsiella pneumoniae
4.1.1.86 L-2,4-diaminobutyrate decarboxylase
-
 Klebsiella oxytoca
4.1.1.86 L-2,4-diaminobutyrate decarboxylase
-
 Citrobacter freundii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.86 37 40
-
 Klebsiella aerogenes
4.1.1.86 37 40
-
 Serratia marcescens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.86 40
-
 stable up to, purified enzyme Klebsiella aerogenes
4.1.1.86 40
-
 stable up to, purified enzyme Serratia marcescens
4.1.1.86 50
-
 above, rapid inactivation Klebsiella aerogenes
4.1.1.86 50
-
 above, rapid inactivation Serratia marcescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.86 8 8.2
-
 Klebsiella aerogenes
4.1.1.86 8 8.2
-
 Serratia marcescens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.1.86 7.2 8.2 narrow pH range, the enzyme is almost inactive at pH 7.0 Klebsiella aerogenes
4.1.1.86 7.2 8.2 narrow pH range, the enzyme is almost inactive at pH 7.0 Serratia marcescens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.1.1.86 6.5 7.5 stable for 12 h at 4°C in 20 mM potassium phosphate Klebsiella aerogenes
4.1.1.86 6.5 7.5 stable for 12 h at 4°C in 20 mM potassium phosphate Serratia marcescens

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.86 pyridoxal 5'-phosphate
-
 Klebsiella aerogenes
4.1.1.86 pyridoxal 5'-phosphate
-
 Serratia marcescens
4.1.1.86 pyridoxal 5'-phosphate
-
 Enterobacter cloacae
4.1.1.86 pyridoxal 5'-phosphate
-
 Pantoea agglomerans
4.1.1.86 pyridoxal 5'-phosphate
-
 Serratia liquefaciens
4.1.1.86 pyridoxal 5'-phosphate
-
 Klebsiella pneumoniae
4.1.1.86 pyridoxal 5'-phosphate
-
 Klebsiella oxytoca
4.1.1.86 pyridoxal 5'-phosphate
-
 Citrobacter freundii