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Literature summary extracted from

  • Rhimi, M.; Bejar, S.
    Cloning, purification and biochemical characterization of metallic-ions independent and thermoactive l-arabinose isomerase from the Bacillus stearothermophilus US100 strain (2006), Biochim. Biophys. Acta, 1760, 191-199.
    View publication on PubMed

Application

EC Number Application Comment Organism
5.3.1.4 food industry production of D-tagatose Geobacillus stearothermophilus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.4 expression in Escherichia coli Geobacillus stearothermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.1.4 Cu2+
-
 Geobacillus stearothermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.4 28.57
-
 L-arabinose
-
 Geobacillus stearothermophilus
5.3.1.4 57
-
 D-galactose
-
 Geobacillus stearothermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.1.4 Co2+ increase in activity at 80°C only, no effect at 65°C Geobacillus stearothermophilus
5.3.1.4 Mn2+ increase in activity at 80°C only, no effect at 65°C Geobacillus stearothermophilus
5.3.1.4 additional information activity and thermostability are totally independent of metallic ions up to 65°C, above 65°C, the enzyme's activity is also independent of metallic ions of its activity, but its thermostability is improved in the presence of only 0.2 mM Co2+ and 1 mM Mn2+ Geobacillus stearothermophilus
5.3.1.4 additional information Ca2+, Ni2+, Zn2+, Mg2+, Fe2+, Cu2+ show no effect Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.4 56000
-
 calculated Geobacillus stearothermophilus
5.3.1.4 56000
-
 monomer, SDS-PAGE Geobacillus stearothermophilus
5.3.1.4 56160
-
 monomer, calculated from amino acid sequence Geobacillus stearothermophilus
5.3.1.4 225000
-
 native gel electrophoresis Geobacillus stearothermophilus
5.3.1.4 225000
-
 tetramer, SDS-PAGE Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.1.4 D-galactose Geobacillus stearothermophilus
-
 D-tagatose
-
 ?
5.3.1.4 D-galactose Geobacillus stearothermophilus US100
-
 D-tagatose
-
 ?
5.3.1.4 L-arabinose Geobacillus stearothermophilus
-
 L-ribulose
-
 ?
5.3.1.4 L-arabinose Geobacillus stearothermophilus US100
-
 L-ribulose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.4 Geobacillus stearothermophilus
-
-
-
5.3.1.4 Geobacillus stearothermophilus Q9S467
-
-
5.3.1.4 Geobacillus stearothermophilus US100
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.4 of the recombinant protein Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.1.4 8.9
-
 with D-galactose as substrate Geobacillus stearothermophilus
5.3.1.4 41.3
-
 with L-arabinose as substrate Geobacillus stearothermophilus
5.3.1.4 185
-
 after purification with FPLC Geobacillus stearothermophilus

Storage Stability

EC Number Storage Stability Organism
5.3.1.4 -20°C in 15% gylcerol Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.4 D-galactose
-
 Geobacillus stearothermophilus D-tagatose
-
 ?
5.3.1.4 D-galactose
-
 Geobacillus stearothermophilus US100 D-tagatose
-
 ?
5.3.1.4 L-arabinose
-
 Geobacillus stearothermophilus L-ribulose
-
 ?
5.3.1.4 L-arabinose
-
 Geobacillus stearothermophilus US100 L-ribulose
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.1.4 tetramer
-
 Geobacillus stearothermophilus
5.3.1.4 tetramer alpha4, 4 * 56000, SDS-PAGE, native gel electrophoresis Geobacillus stearothermophilus

Synonyms

EC Number Synonyms Comment Organism
5.3.1.4 D-galactose isomerase
-
 Geobacillus stearothermophilus
5.3.1.4 L-AI US100
-
 Geobacillus stearothermophilus
5.3.1.4 L-arabinose aldose-ketose-isomerase
-
 Geobacillus stearothermophilus
5.3.1.4 L-arabinose isomerase
-
 Geobacillus stearothermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.1.4 70
-
 for conversion of D-galactose Geobacillus stearothermophilus
5.3.1.4 80
-
-
 Geobacillus stearothermophilus
5.3.1.4 80
-
 for conversion of L-arabinose Geobacillus stearothermophilus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5.3.1.4 60 90
-
 Geobacillus stearothermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.3.1.4 additional information
-
 thermostability is totally independent of metallic ions up to 65°C, above 65°C, thermostability is improved in the presence of only 0.2 mM Co2+ and 1 mM Mn2+ Geobacillus stearothermophilus
5.3.1.4 50 65 100% activity in absence of metallic ions during 12 h Geobacillus stearothermophilus
5.3.1.4 65
-
 until 65°C independent from metallic ions, above 65°C stability is improved in presence of 0.2 mM Co2+ and 1 mM Mn2+ Geobacillus stearothermophilus
5.3.1.4 70 80 decrease in activity in absence of metallic ions Geobacillus stearothermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.4 7.5
-
-
 Geobacillus stearothermophilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.3.1.4 7 9
-
 Geobacillus stearothermophilus