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Literature summary extracted from

  • de Atauri, P.; Repiso, A.; Oliva, B.; Vives-Corrons, J.L.; Climent, F.; Carreras, J.
    Characterization of the first described mutation of human red blood cell phosphoglycerate mutase (2005), Biochim. Biophys. Acta, 1740, 403-410.
    View publication on PubMed

Application

EC Number Application Comment Organism
5.4.2.11 medicine mutation M230I is identified in a patient with hereditary sperocytosis and 50% deficiency of enzyme activity in red blood cells. Enzyme shows abnormal behaviour on ion-exchange chromatography and is more thermo-labile than wild-type Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
5.4.2.11 M230I mutation identified in a patient with hereditary sperocytosis and 50% deficiency of enzyme activity in red blood cells. Enzyme shows abnormal behaviour on ion-exchange chromatography and is more thermo-labile than wild-type Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4.2.11 0.4
-
 3-phospho-D-glycerate pH 7.4, 30°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
5.4.2.11 Homo sapiens
-
 patient with hereditary sperocytosis and 50% deficiency of enzyme activity in red blood cells
-

Reaction

EC Number Reaction Comment Organism Reaction ID
5.4.2.11 2-phospho-D-glycerate = 3-phospho-D-glycerate ping-pong or phosphoenzyme mechanism Homo sapiens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.4.2.11 erythrocyte hemoglobin-free hemolysate Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.2.11 2-phospho-D-glycerate
-
 Homo sapiens 3-phospho-D-glycerate
-
 r
5.4.2.11 3-phospho-D-glycerate
-
 Homo sapiens 2-phospho-D-glycerate
-
 r
5.4.2.11 additional information enzyme possesses some 2,3-bisphosphoglycerate phosphatase activity Homo sapiens ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.4.2.11 45
-
 wild-type, 1 h, 10% loss of activity, mutant M230I, 20 min, 50% loss of activity, 1 h, 90% loss of activity. In presence of 2,3-bisphosphoglycerate, both forms remain stable Homo sapiens