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Literature summary extracted from

  • Lu, J.; Tong, J.; Feng, H.; Huang, J.; Afonso, C.L.; Rock, D.L.; Barany, F.; Cao, W.
    Unique ligation properties of eukaryotic NAD+-dependent DNA ligase from Melanoplus sanguinipes entomopoxvirus (2004), Biochim. Biophys. Acta, 1701, 37-48.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.5.1.2 expression in Escherichia coli Melanoplus sanguinipes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.5.1.2 0.000136
-
 DNA pH 7.6, 21°C, C/G matched nick DNA substrate Melanoplus sanguinipes
6.5.1.2 0.0058
-
 NAD+ pH 7.6, 21°C Melanoplus sanguinipes

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.5.1.2 Mg2+ or Mn2+ required, maximal activity at 10-15 mM Melanoplus sanguinipes
6.5.1.2 Mn2+ or Mg2+ required, maximal activity at 1 mM Melanoplus sanguinipes

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.2 Melanoplus sanguinipes
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.5.1.2 recombinant enzyme Melanoplus sanguinipes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.2 ATP + DNA
-
 Melanoplus sanguinipes ?
-
 ?
6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m the enzyme seals sticky ends efficiently, but has little activity on 1-nucleotide gap or blunt-ended DNA substrates even in presence of polyethylene glycol. MsEPV DNA ligase readily joins DNA nicks with mismatches at either side of the nick junction, except for mismatches at the nick junction containing an A base in the template strand (A/A, G/A, and C/A). The enzyme can join DNA probes on RNA templates. MsEPV DNA ligase joins mismatches at the 3' side of the nick more frequently than at the 5' side of the nick on an RNA template. No activity with ATP Melanoplus sanguinipes AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.5.1.2 MsEPV DNA ligase
-
 Melanoplus sanguinipes

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.5.1.2 37
-
-
 Melanoplus sanguinipes

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
6.5.1.2 31 45 about 70% of maximal activity at 31°C and at 45°C Melanoplus sanguinipes

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.5.1.2 0.4
-
 DNA pH 7.6, 21°C, C/G matched nick DNA substrate Melanoplus sanguinipes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5.1.2 9
-
-
 Melanoplus sanguinipes

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.5.1.2 7.7 9.7 about 70% of maximal activity at pH 7.7-9.7 Melanoplus sanguinipes

Cofactor

EC Number Cofactor Comment Organism Structure
6.5.1.2 additional information no activity with ATP Melanoplus sanguinipes
6.5.1.2 NAD+
-
 Melanoplus sanguinipes